Original Article

An Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Linguatula serrata in Experimentally Infected Dogs


Background: Linguatula serrata is a causative agent of visceral and nasopharyngeal linguatulosis in humans and animals. The aim of the present study was to investigate the immune response of dogs experimentally infected by L. serrata with ELISA.

Methods: Five puppies were infected by inserting the L. serrata nymphs in their nasal cavities (infected group) in the Department of Parasitology of Shahid Chamran University of Ahvaz, during 2018-2019. Three animals were kept as the non-infected control group. Blood samples were collected from the animals for seven months at approximately monthly intervals for serum preparation. Nasal samples were taken weekly from the fourth month. ELISA was designed and performed on 64 sera (24 negatives, and 40 positives) using somatic (S), and excretory-secretory (ES) antigens.

Results: Overall, 100% of the animals were infected with the parasite. Based on the results of ELISA, the ES antigen (sensitivity 95% and specificity 92%) was more preferred than the S antigen (sensitivity 95% and specificity 85%). Female parasites had significant effects on the immune response. There was a significant correlation between the clinical symptoms and the presence of female parasites (P<0.05).

Conclusion: The results showed a practical method for dogs' experimental infection. ELISA method is suitable for the detection of infection at different stages of development, especially before the maturation stage of the parasite. In this regard, the ES antigen of the parasite was more immunogenic. Therefore, ELISA can be used as a serological method in the early detection and epidemiological studies of infection with L. serrata in dogs.

1. Marquardt WC, Demaree RS, Grieve RB. Parasitology and Vector Biology. 2nd ed, London: Harcourt/ Academic Press; 2000. P. 511–513.
2. Mehlhorn H. Encyclopedia of Parasitology. 3rd ed, Heidelberg: Springer-Verlag; 2008. P.720-722.
3. Meshgi B, Asgarian O. Prevalence of Linguatula serrata infestation in stray dogs of Shahrekord, Iran. J Vet Med B Infect Dis Vet Public Health. 2003; 50(9):466-7.
4. Oryan A, Sadjjadi S, Mehrabani D, et al. The status of Linguatula serrata infection of stray dogs in Shiraz, Iran. Comp Clin Pathol. 2008; 17(1):55-60.
5. Razmaraei N, Ameghi A, Ebrahimi M, et al. A study of the infection rate of linguatula serrata in stray dogs in Marand. Iran Vet J. 2007: 3 (16):100 - 105.
6. Bahrami A, Yousofzadeh S, Kermanjani A. Study of Linguatula serrata infection rate among shepherd and stray dogs in Ilam (Western Iran). SJIMU. 2011; 19: 60-5. ISSN: 1563-4728.
7. Rezaei F, Tavassoli M, Mahmoudian A. Prevalence of Linguatula serrata infection among dogs (definitive host) and domestic ruminants (intermediate host) in the North West of Iran. Vet Med-Czech. 2011; 56 (11): 561–567.
8. Windsor RC, Johnson LR. Canine chronic inflammatory rhinitis. Clin Tech Small Anim Pract. 2006; 21(2):76-81.
9. Smith J, Oladiran B, Lagundoye S, et al. Pentastomiasis and malignancy. Ann Trop Med Parasitol. 1975; 69(4):503-12.
10. Bordicchia M, Falcioni D, Scarpona S, et al. Nasal carcinoma in a dog with Linguatula serrata infection. Vet. Rec. Case Rep. 2014; 2(1):e000015.
11. Akyol C, Coskun S, Sonmez G, et al. Linguatula serrata infection in Bursa stray dogs and its importance from the point of public health. Parazitol Derg. 1995; 19:267-71.
12. Beaver P, Jung R, Cupp E. Clinical Parasitology 9th ed Lea & Febiger. Philadelphia; 1984. p. 162-167.
13. Khalil GM. Linguatula serrata from mongrel dogs in El-Dakhla Oasis (Egypt). J Parasitol. 1973; 59(2):288.
14. Oluwasina OS, ThankGod OE, Augustine OO et al. Linguatula serrata (Porocephalida: Linguatulidae) infection among client-owned dogs in Jalingo, North Eastern Nigeria: prevalence and public health implications. J Parasitol Res. 2014; 2014.
15. Villedieu E, Sanchez RF, Jepson RE et al. Nasal infestation by Linguatula serrata in a dog in the UK: a case report. J Small Anim Pract. 2017; 58(3):183-6.
16. Attia MM, Ismael E, Saleh NMK. A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis. Vet World. 2019; 12(6): 883-889.
17. Angulo-Valadez C, Cepeda-Palacios R, Ascencio F et al. IgG antibody response against salivary gland antigens from Oestrus ovis L. larvae (Diptera: Oestridae) in experimentally and naturally infected goats. Vet Parasitol. 2009; 161(3-4):356-9.
18. Alborzi A, Ghorbanpoor M, Hamidinejat H et al. Development and evaluation of an indirect ELISA test for serodiagnosis of Linguatula serrata infection in sheep. Iran J Vet Res. 2015; 11(1):23-33.
19. Angulo-Valadez CE, Scala A, Grisez C et al. Specific IgG antibody responses in Oestrus ovis L.(Diptera: Oestridae) infected sheep: associations with intensity of infection and larval development. Vet Parasitol. 2008; 155(3-4):257-63.
20. Angulo‐Valadez C, Ascencio F, Jacquiet P et al. Sheep and goat immune responses to nose bot infestation: a review. Med Vet Entomol. 2011; 25(2):117-25.
21. Silva BF, Bassetto CC, Amarante AF. Immune humoral response of young lambs naturally infested by Oestrus ovis (Diptera: Oestridae). Rev Bras Parasitol Vet. 2018; 27(3):295-300.
22. Silva BF, Bassetto CC, Amarante AF. Immune responses in sheep naturally infected with Oestrus ovis (Diptera: Oestridae) and gastrointestinal nematodes. Vet Parasitol. 2012; 190(1-2):120-6.
23. Romero J, Arias M, Suarez J et al. Application of the analysis of serum antibodies (immunoglobulins M and G) to estimate the seroprevalence of ovine oestrosis and to evaluate the effect of chemotherapy. J Med Entomol. 2010; 47(3):477-81.
24. Suárez J, Scala A, Romero J et al. Analysis of the humoral immune response to Oestrus ovis in ovine. Vet Parasitol. 2005; 134(1-2):153-8.
25. Van Pelt DR, McKiernan BC. Pathogenesis and Treatment of Canine Rhinitis. Vet Clin North Am Small Anim Pract. 1994; 24(5):789-806.
IssueVol 16 No 3 (2021) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijpa.v16i3.7093
Linguatula serrata Experimental infection Dog

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How to Cite
Alborzi A, Pirkoreshk J, Bahrami S, Ghorbanpoor M, Tabandeh MR. An Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Linguatula serrata in Experimentally Infected Dogs. Iran J Parasitol. 2021;16(3):402-410.