Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococ-cosis in Baqiyatallah Hospital, Tehran, Iran
-
Hamidreza NEYSI
,
Tahereh MOHAMMADZADEH
,
Seyed Mahmoud SADJJADI
,
Jamal AKHAVANMOGHADDAM
,
Alireza SHAMSAEI
Abstract
Background: Cystic echinococcosis (CE) is a worldwide zoonotic helminthic disease caused by the larval stage of Echinococcus granulosus. The infection is particularly important in terms of economic and medico-veterinary aspects in endemic areas including Iran. Considering the possibility of organ-tropism in E. granulosus strains, the present study was aimed to identify the genotypes of E. granulosus in different organs involved in patients, undergone surgery in Baqiyatallah Hospital, Tehran, Iran from 2005-2015.
Methods: Overall, 29 formalin-fixed paraffin-embedded tissues (FFPT) from patients with histologically confirmed CE including liver (N: 14) lungs (N: 6) abdomen (N: 2), pancreas (N: 2) and each of spleen, gallbladder and, muscles (N: 1) plus unknown organs (N: 2) were used and genetically characterized using polymerase chain reaction, followed by partial sequencing of mitochondrial cytochrome c oxidase gene subunit 1(cox1) and analyzed.
Results: Nineteen out of 29 isolates including liver (N: 6) lungs (N: 4) abdomen (N: 2), pancreas (N: 2) and each of spleen, gallbladder, and muscle (N: 1), unknown organs (N: 2) obtained from paraffin-embedded blocks of human CE created an acceptable sequence in two directions. All 19 isolates regardless of the organ involved were recognized as E. granulosus sensu stricto (G1).
Conclusion: The sequence alignments of the isolates displayed two profiles. All sequenced samples showed E. granulosus sensu stricto (G1) with no organ-related genotype.
1. Deplazes P, Rinaldi L, Alvarez Rojas CA et al. Global Distribution of Alveolar and Cystic Echino-coccosis. Adv Parasitol. 2017; 95:315-493.
2. Rokni M. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009; 4(2): 1-16.
3. Fallah M, Taherkhani H, Sadjjadi M. Echinococ-cosis in stray dogs in Hamedan, west of Iran. Iran J Med Sci. 1995; 29:170-72.
4. Alvarez Rojas CA, Romig T, Lightowlers MW. Echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge. Int J Parasi-tol. 2014; 44(1):9-18.
5. Sadjjadi SM, Sedaghat F, Hosseini SV et al. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol. 2009; 47(2):153-57.
6. Mohammadzadeh T, Sako Y, Sadjjadi SM et al. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg. 2012; 106(6):371-75.
7. Khalilpour A, Sadjjadi SM, Moghadam ZK et al. Lateral flow test using Echinococcus granulosus native antigen B and comparison of IgG and IgG4 dip-sticks for detection of human cystic echinococ-cosis. Am J Trop Med Hyg. 2014; 91(5):994-99.
8. Sadjjadi FS, Rezaie-Tavirani M, Ahmadi NA et al. Proteome evaluation of human cystic echinococ-cosis sera using two dimensional gel electrophore-sis. Gastroenterol Hepatol Bed Bench. 2018; 11(1):75-82.
9. Babba H, Messedi A, Masmoudi S et al. Diagnosis of human hydatidosis - comparison between im-agery and 6 serologic techniques. Am J Trop Med Hyg. 1994; 50(1): 64–68.
10. Gökçe O, Gökçe C, Yilmaz M et al. Povidone iodine in experimental peritoneal hydatidosis. Br J Surg. 1991; 78(4):495-96.
11. Sadjjadi SM, Zoharizadeh MR, Panjeshahin MR. In vitro screening of different Allium sativum extracts on hydatid cysts protoscoleces. J Invest Surg. 2008; 21(6):318-22.
12. Hosseini SV, Ghanbarzadeh K, Barzin J et al. In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst. Korean J Parasitol. 2006; 44(3):239-42.
13. Tabatabaei ZS, Dehshahri S, Taghi MM et al. In vitro study on protoscolicidal effect of methanolic extract of Allium hirtifolium on protoscoleces of cyst-ic echinococcosis. Infect Disord Drug Targets. 2018;9 [Epub ahead of print].
14. Sadjjadi SM, Ardehali S, Noman-Pour B et al. Di-agnosis of cystic echinococcosis: ultrasound imag-ing or countercurrent immunoelectrophoresis? East Mediterr Health J. 2001; 7(6):907-11.
15. Yanagida T, Mohammadzadeh T, Kamhawi S et al. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int. 2012; 61(4):599-603.
16. Sadjjadi SM, Mikaeili F, Karamian M et al. Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013; 43(11):875-77.
17. Sadjjadi SM, Ebrahimipour M, Sadjjadi FS. Com-parison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1and nad1) and their related protein models us-ing experimental and bioinformatics analysis. Com-put Biol Chem. 2019;79 : 103-109.
18. Gholami S, Jahandar H, Abastabar M et al. Echino-coccus granulosus Sensu stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran. Iran J Parasitol. 2016; 11(2):186-94.
19. Hosseini-Safa A, Mohaghegh MA, Pestechian N et al. First report of Tasmanian sheep strain (G2) gen-otype isolated from Iranian goat using the high resolution melting (HRM) analysis. Gastroenterol Hepatol Bed Bench. 2016; 9(Suppl1):S70-S74.
20. Ebrahimipour M, Sadjjadi S.M, Yousofi Darani H et al. Molecular Studies on Cystic Echinococcosis of camel (Camelus dromedarius) and report of Echinococcus ortleppi in Iran. Iran J Parasitol. 2017; 12(3):323-31.
21. Karamian M, Haghighi F, Hemmati M et al. Heter-ogenity of Echinococcus canadensis genotype 6 - the main causative agent of cystic echinococcosis in Birjand, Eastern Iran.Vet Parasitol. 2017; 245:78-85.
22. Bowles J, Blair D, McManus DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54(2):165-173.
23. Hall, T. BioEdit version 5.0.6. 2001; http://darwin.uvigo.es/dposada/repository/pdfs/BioEdit.help.pdf
24. Tamura K, Peterson D, Peterson N,et al. MEGA5: molecular evolutionary genetics analysis using max-imum likelihood, evolutionary distance, and maxi-mum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-39.
25. Grosso G, Gruttadauria S, Biondi A, et al. World-wide epidemiology of liver hydatidosis including the Mediterranean area. World J Gastroenterol. 2012; 18(13):1425-37.
26. Thompson RCA, McManus. Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002; 18(10):452-57.
27. Nakao M, McManus D, Schantz P, et al. A mo-lecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitol. 2007; 134(Pt 5):713-22.
28. Sharbatkhori M, Mirhendi H, Jex AR et al. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning-phylogenetic approach. Electro-phoresis. 2009; 30(15):2648-55.
29. Rostami S, Torbaghan SS, Dabiri S et al. Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tis-sue samples of human isolates in Iran. Am J Trop Med Hyg. 2015; 92(3):588-94.
30. Khademvatan S, Yousefi E, Rafiei A, et al. Molecu-lar characterization of livestock and human isolates of Echinococcus granulosus from south-west Iran. J Helminthol. 2013; 87(2):240-44.
31. Kia EB, Rahimi H, Sharbatkhori M et al. Genotype identification of human cystic echinococcosis in Is-fahan, central Iran. Parasitol Res. 2010; 107(3):757-60.
32. Hedayati Z, Daryani A, Sarvi S et al. Molecular Genotyping of the Human Cystic Echinococcosis in Mazandaran Province, North of Iran. Iran J Para-sitol. 2019; 14(1): 151–158.
33. Nikmanesh B, Mirhendi H, Ghalavand Z et al. Genotyping of Echinococcus granulosus Isolates from Human Clinical Samples Based on Sequencing of Mitochondrial Genes in Iran, Tehran. Iran J Parasi-tol. 2014; 9(1):20-27.
34. Hassan ZI, Meerkhan AA, Boufana B et al. Two haplotype clusters of Echinococcus granulosus sensu stricto in northern Iraq (Kurdistan region) support the hypothesis of a parasite cradle in the Middle East. Acta Trop. 2017; 172:201-207.
35. Nakao M, Xiao N, Okamoto M et al. Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis. Parasitol Int. 2009; 58(4):384-9.
1. Deplazes P, Rinaldi L, Alvarez Rojas CA et al. Global Distribution of Alveolar and Cystic Echino-coccosis. Adv Parasitol. 2017; 95:315-493.
2. Rokni M. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009; 4(2): 1-16.
3. Fallah M, Taherkhani H, Sadjjadi M. Echinococ-cosis in stray dogs in Hamedan, west of Iran. Iran J Med Sci. 1995; 29:170-72.
4. Alvarez Rojas CA, Romig T, Lightowlers MW. Echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge. Int J Parasi-tol. 2014; 44(1):9-18.
5. Sadjjadi SM, Sedaghat F, Hosseini SV et al. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol. 2009; 47(2):153-57.
6. Mohammadzadeh T, Sako Y, Sadjjadi SM et al. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg. 2012; 106(6):371-75.
7. Khalilpour A, Sadjjadi SM, Moghadam ZK et al. Lateral flow test using Echinococcus granulosus native antigen B and comparison of IgG and IgG4 dip-sticks for detection of human cystic echinococ-cosis. Am J Trop Med Hyg. 2014; 91(5):994-99.
8. Sadjjadi FS, Rezaie-Tavirani M, Ahmadi NA et al. Proteome evaluation of human cystic echinococ-cosis sera using two dimensional gel electrophore-sis. Gastroenterol Hepatol Bed Bench. 2018; 11(1):75-82.
9. Babba H, Messedi A, Masmoudi S et al. Diagnosis of human hydatidosis - comparison between im-agery and 6 serologic techniques. Am J Trop Med Hyg. 1994; 50(1): 64–68.
10. Gökçe O, Gökçe C, Yilmaz M et al. Povidone iodine in experimental peritoneal hydatidosis. Br J Surg. 1991; 78(4):495-96.
11. Sadjjadi SM, Zoharizadeh MR, Panjeshahin MR. In vitro screening of different Allium sativum extracts on hydatid cysts protoscoleces. J Invest Surg. 2008; 21(6):318-22.
12. Hosseini SV, Ghanbarzadeh K, Barzin J et al. In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst. Korean J Parasitol. 2006; 44(3):239-42.
13. Tabatabaei ZS, Dehshahri S, Taghi MM et al. In vitro study on protoscolicidal effect of methanolic extract of Allium hirtifolium on protoscoleces of cyst-ic echinococcosis. Infect Disord Drug Targets. 2018;9 [Epub ahead of print].
14. Sadjjadi SM, Ardehali S, Noman-Pour B et al. Di-agnosis of cystic echinococcosis: ultrasound imag-ing or countercurrent immunoelectrophoresis? East Mediterr Health J. 2001; 7(6):907-11.
15. Yanagida T, Mohammadzadeh T, Kamhawi S et al. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int. 2012; 61(4):599-603.
16. Sadjjadi SM, Mikaeili F, Karamian M et al. Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013; 43(11):875-77.
17. Sadjjadi SM, Ebrahimipour M, Sadjjadi FS. Com-parison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1and nad1) and their related protein models us-ing experimental and bioinformatics analysis. Com-put Biol Chem. 2019;79 : 103-109.
18. Gholami S, Jahandar H, Abastabar M et al. Echino-coccus granulosus Sensu stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran. Iran J Parasitol. 2016; 11(2):186-94.
19. Hosseini-Safa A, Mohaghegh MA, Pestechian N et al. First report of Tasmanian sheep strain (G2) gen-otype isolated from Iranian goat using the high resolution melting (HRM) analysis. Gastroenterol Hepatol Bed Bench. 2016; 9(Suppl1):S70-S74.
20. Ebrahimipour M, Sadjjadi S.M, Yousofi Darani H et al. Molecular Studies on Cystic Echinococcosis of camel (Camelus dromedarius) and report of Echinococcus ortleppi in Iran. Iran J Parasitol. 2017; 12(3):323-31.
21. Karamian M, Haghighi F, Hemmati M et al. Heter-ogenity of Echinococcus canadensis genotype 6 - the main causative agent of cystic echinococcosis in Birjand, Eastern Iran.Vet Parasitol. 2017; 245:78-85.
22. Bowles J, Blair D, McManus DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54(2):165-173.
23. Hall, T. BioEdit version 5.0.6. 2001; http://darwin.uvigo.es/dposada/repository/pdfs/BioEdit.help.pdf
24. Tamura K, Peterson D, Peterson N,et al. MEGA5: molecular evolutionary genetics analysis using max-imum likelihood, evolutionary distance, and maxi-mum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-39.
25. Grosso G, Gruttadauria S, Biondi A, et al. World-wide epidemiology of liver hydatidosis including the Mediterranean area. World J Gastroenterol. 2012; 18(13):1425-37.
26. Thompson RCA, McManus. Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002; 18(10):452-57.
27. Nakao M, McManus D, Schantz P, et al. A mo-lecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitol. 2007; 134(Pt 5):713-22.
28. Sharbatkhori M, Mirhendi H, Jex AR et al. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning-phylogenetic approach. Electro-phoresis. 2009; 30(15):2648-55.
29. Rostami S, Torbaghan SS, Dabiri S et al. Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tis-sue samples of human isolates in Iran. Am J Trop Med Hyg. 2015; 92(3):588-94.
30. Khademvatan S, Yousefi E, Rafiei A, et al. Molecu-lar characterization of livestock and human isolates of Echinococcus granulosus from south-west Iran. J Helminthol. 2013; 87(2):240-44.
31. Kia EB, Rahimi H, Sharbatkhori M et al. Genotype identification of human cystic echinococcosis in Is-fahan, central Iran. Parasitol Res. 2010; 107(3):757-60.
32. Hedayati Z, Daryani A, Sarvi S et al. Molecular Genotyping of the Human Cystic Echinococcosis in Mazandaran Province, North of Iran. Iran J Para-sitol. 2019; 14(1): 151–158.
33. Nikmanesh B, Mirhendi H, Ghalavand Z et al. Genotyping of Echinococcus granulosus Isolates from Human Clinical Samples Based on Sequencing of Mitochondrial Genes in Iran, Tehran. Iran J Parasi-tol. 2014; 9(1):20-27.
34. Hassan ZI, Meerkhan AA, Boufana B et al. Two haplotype clusters of Echinococcus granulosus sensu stricto in northern Iraq (Kurdistan region) support the hypothesis of a parasite cradle in the Middle East. Acta Trop. 2017; 172:201-207.
35. Nakao M, Xiao N, Okamoto M et al. Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis. Parasitol Int. 2009; 58(4):384-9.
2. Rokni M. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009; 4(2): 1-16.
3. Fallah M, Taherkhani H, Sadjjadi M. Echinococ-cosis in stray dogs in Hamedan, west of Iran. Iran J Med Sci. 1995; 29:170-72.
4. Alvarez Rojas CA, Romig T, Lightowlers MW. Echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge. Int J Parasi-tol. 2014; 44(1):9-18.
5. Sadjjadi SM, Sedaghat F, Hosseini SV et al. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol. 2009; 47(2):153-57.
6. Mohammadzadeh T, Sako Y, Sadjjadi SM et al. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg. 2012; 106(6):371-75.
7. Khalilpour A, Sadjjadi SM, Moghadam ZK et al. Lateral flow test using Echinococcus granulosus native antigen B and comparison of IgG and IgG4 dip-sticks for detection of human cystic echinococ-cosis. Am J Trop Med Hyg. 2014; 91(5):994-99.
8. Sadjjadi FS, Rezaie-Tavirani M, Ahmadi NA et al. Proteome evaluation of human cystic echinococ-cosis sera using two dimensional gel electrophore-sis. Gastroenterol Hepatol Bed Bench. 2018; 11(1):75-82.
9. Babba H, Messedi A, Masmoudi S et al. Diagnosis of human hydatidosis - comparison between im-agery and 6 serologic techniques. Am J Trop Med Hyg. 1994; 50(1): 64–68.
10. Gökçe O, Gökçe C, Yilmaz M et al. Povidone iodine in experimental peritoneal hydatidosis. Br J Surg. 1991; 78(4):495-96.
11. Sadjjadi SM, Zoharizadeh MR, Panjeshahin MR. In vitro screening of different Allium sativum extracts on hydatid cysts protoscoleces. J Invest Surg. 2008; 21(6):318-22.
12. Hosseini SV, Ghanbarzadeh K, Barzin J et al. In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst. Korean J Parasitol. 2006; 44(3):239-42.
13. Tabatabaei ZS, Dehshahri S, Taghi MM et al. In vitro study on protoscolicidal effect of methanolic extract of Allium hirtifolium on protoscoleces of cyst-ic echinococcosis. Infect Disord Drug Targets. 2018;9 [Epub ahead of print].
14. Sadjjadi SM, Ardehali S, Noman-Pour B et al. Di-agnosis of cystic echinococcosis: ultrasound imag-ing or countercurrent immunoelectrophoresis? East Mediterr Health J. 2001; 7(6):907-11.
15. Yanagida T, Mohammadzadeh T, Kamhawi S et al. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int. 2012; 61(4):599-603.
16. Sadjjadi SM, Mikaeili F, Karamian M et al. Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013; 43(11):875-77.
17. Sadjjadi SM, Ebrahimipour M, Sadjjadi FS. Com-parison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1and nad1) and their related protein models us-ing experimental and bioinformatics analysis. Com-put Biol Chem. 2019;79 : 103-109.
18. Gholami S, Jahandar H, Abastabar M et al. Echino-coccus granulosus Sensu stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran. Iran J Parasitol. 2016; 11(2):186-94.
19. Hosseini-Safa A, Mohaghegh MA, Pestechian N et al. First report of Tasmanian sheep strain (G2) gen-otype isolated from Iranian goat using the high resolution melting (HRM) analysis. Gastroenterol Hepatol Bed Bench. 2016; 9(Suppl1):S70-S74.
20. Ebrahimipour M, Sadjjadi S.M, Yousofi Darani H et al. Molecular Studies on Cystic Echinococcosis of camel (Camelus dromedarius) and report of Echinococcus ortleppi in Iran. Iran J Parasitol. 2017; 12(3):323-31.
21. Karamian M, Haghighi F, Hemmati M et al. Heter-ogenity of Echinococcus canadensis genotype 6 - the main causative agent of cystic echinococcosis in Birjand, Eastern Iran.Vet Parasitol. 2017; 245:78-85.
22. Bowles J, Blair D, McManus DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54(2):165-173.
23. Hall, T. BioEdit version 5.0.6. 2001; http://darwin.uvigo.es/dposada/repository/pdfs/BioEdit.help.pdf
24. Tamura K, Peterson D, Peterson N,et al. MEGA5: molecular evolutionary genetics analysis using max-imum likelihood, evolutionary distance, and maxi-mum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-39.
25. Grosso G, Gruttadauria S, Biondi A, et al. World-wide epidemiology of liver hydatidosis including the Mediterranean area. World J Gastroenterol. 2012; 18(13):1425-37.
26. Thompson RCA, McManus. Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002; 18(10):452-57.
27. Nakao M, McManus D, Schantz P, et al. A mo-lecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitol. 2007; 134(Pt 5):713-22.
28. Sharbatkhori M, Mirhendi H, Jex AR et al. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning-phylogenetic approach. Electro-phoresis. 2009; 30(15):2648-55.
29. Rostami S, Torbaghan SS, Dabiri S et al. Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tis-sue samples of human isolates in Iran. Am J Trop Med Hyg. 2015; 92(3):588-94.
30. Khademvatan S, Yousefi E, Rafiei A, et al. Molecu-lar characterization of livestock and human isolates of Echinococcus granulosus from south-west Iran. J Helminthol. 2013; 87(2):240-44.
31. Kia EB, Rahimi H, Sharbatkhori M et al. Genotype identification of human cystic echinococcosis in Is-fahan, central Iran. Parasitol Res. 2010; 107(3):757-60.
32. Hedayati Z, Daryani A, Sarvi S et al. Molecular Genotyping of the Human Cystic Echinococcosis in Mazandaran Province, North of Iran. Iran J Para-sitol. 2019; 14(1): 151–158.
33. Nikmanesh B, Mirhendi H, Ghalavand Z et al. Genotyping of Echinococcus granulosus Isolates from Human Clinical Samples Based on Sequencing of Mitochondrial Genes in Iran, Tehran. Iran J Parasi-tol. 2014; 9(1):20-27.
34. Hassan ZI, Meerkhan AA, Boufana B et al. Two haplotype clusters of Echinococcus granulosus sensu stricto in northern Iraq (Kurdistan region) support the hypothesis of a parasite cradle in the Middle East. Acta Trop. 2017; 172:201-207.
35. Nakao M, Xiao N, Okamoto M et al. Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis. Parasitol Int. 2009; 58(4):384-9.
1. Deplazes P, Rinaldi L, Alvarez Rojas CA et al. Global Distribution of Alveolar and Cystic Echino-coccosis. Adv Parasitol. 2017; 95:315-493.
2. Rokni M. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009; 4(2): 1-16.
3. Fallah M, Taherkhani H, Sadjjadi M. Echinococ-cosis in stray dogs in Hamedan, west of Iran. Iran J Med Sci. 1995; 29:170-72.
4. Alvarez Rojas CA, Romig T, Lightowlers MW. Echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge. Int J Parasi-tol. 2014; 44(1):9-18.
5. Sadjjadi SM, Sedaghat F, Hosseini SV et al. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol. 2009; 47(2):153-57.
6. Mohammadzadeh T, Sako Y, Sadjjadi SM et al. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg. 2012; 106(6):371-75.
7. Khalilpour A, Sadjjadi SM, Moghadam ZK et al. Lateral flow test using Echinococcus granulosus native antigen B and comparison of IgG and IgG4 dip-sticks for detection of human cystic echinococ-cosis. Am J Trop Med Hyg. 2014; 91(5):994-99.
8. Sadjjadi FS, Rezaie-Tavirani M, Ahmadi NA et al. Proteome evaluation of human cystic echinococ-cosis sera using two dimensional gel electrophore-sis. Gastroenterol Hepatol Bed Bench. 2018; 11(1):75-82.
9. Babba H, Messedi A, Masmoudi S et al. Diagnosis of human hydatidosis - comparison between im-agery and 6 serologic techniques. Am J Trop Med Hyg. 1994; 50(1): 64–68.
10. Gökçe O, Gökçe C, Yilmaz M et al. Povidone iodine in experimental peritoneal hydatidosis. Br J Surg. 1991; 78(4):495-96.
11. Sadjjadi SM, Zoharizadeh MR, Panjeshahin MR. In vitro screening of different Allium sativum extracts on hydatid cysts protoscoleces. J Invest Surg. 2008; 21(6):318-22.
12. Hosseini SV, Ghanbarzadeh K, Barzin J et al. In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst. Korean J Parasitol. 2006; 44(3):239-42.
13. Tabatabaei ZS, Dehshahri S, Taghi MM et al. In vitro study on protoscolicidal effect of methanolic extract of Allium hirtifolium on protoscoleces of cyst-ic echinococcosis. Infect Disord Drug Targets. 2018;9 [Epub ahead of print].
14. Sadjjadi SM, Ardehali S, Noman-Pour B et al. Di-agnosis of cystic echinococcosis: ultrasound imag-ing or countercurrent immunoelectrophoresis? East Mediterr Health J. 2001; 7(6):907-11.
15. Yanagida T, Mohammadzadeh T, Kamhawi S et al. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int. 2012; 61(4):599-603.
16. Sadjjadi SM, Mikaeili F, Karamian M et al. Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013; 43(11):875-77.
17. Sadjjadi SM, Ebrahimipour M, Sadjjadi FS. Com-parison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1and nad1) and their related protein models us-ing experimental and bioinformatics analysis. Com-put Biol Chem. 2019;79 : 103-109.
18. Gholami S, Jahandar H, Abastabar M et al. Echino-coccus granulosus Sensu stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran. Iran J Parasitol. 2016; 11(2):186-94.
19. Hosseini-Safa A, Mohaghegh MA, Pestechian N et al. First report of Tasmanian sheep strain (G2) gen-otype isolated from Iranian goat using the high resolution melting (HRM) analysis. Gastroenterol Hepatol Bed Bench. 2016; 9(Suppl1):S70-S74.
20. Ebrahimipour M, Sadjjadi S.M, Yousofi Darani H et al. Molecular Studies on Cystic Echinococcosis of camel (Camelus dromedarius) and report of Echinococcus ortleppi in Iran. Iran J Parasitol. 2017; 12(3):323-31.
21. Karamian M, Haghighi F, Hemmati M et al. Heter-ogenity of Echinococcus canadensis genotype 6 - the main causative agent of cystic echinococcosis in Birjand, Eastern Iran.Vet Parasitol. 2017; 245:78-85.
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| Files | ||
| Issue | Vol 15 No 1 (2020) | |
| Section | Short Communication(s) | |
| DOI | https://doi.org/10.18502/ijpa.v15i1.2536 | |
| Keywords | ||
| Genetic characteriza-tion Echinococcus granulosus Paraffin-embedded tissue Iran | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
NEYSI H, MOHAMMADZADEH T, SADJJADI SM, AKHAVANMOGHADDAM J, SHAMSAEI A. Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococ-cosis in Baqiyatallah Hospital, Tehran, Iran. Iran J Parasitol. 2020;15(1):130-137.
