Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hos-pitals, Central Iran, 2015-16

  • Shahrokh IZADI Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mohammad Ali MOHAGHEGH Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran .Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
  • Zahra GHAYOUR-NAJAFABADI Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mehdi AZAMI Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  • Farzaneh MIRZAEI Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran .Department of Laboratory Sciences, School of Para-Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Fatemeh NAMDAR Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mehdi MOHEBALI Department of Medical Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran .Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
  • Dhammika LESHAN WANNIGAMA Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Bangkok, Thailand .Davison of Pediatrics, School of Medicine, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia
  • Seyed Hossein HEJAZI Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran .Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Keywords:
Cryptosporidium, Immunocompromised, patients, Genotype, Iran

Abstract

Background: The purpose of this study was to determine the prevalence and genotype of Cryptosporidium spp. in different groups of immunocompromised patients admitted to the referral hospitals in center of Iran during 2015–2016. Methods: This cross-sectional study was conducted on 346 immunocompromised patients (HIV+/AIDS, Lymphoma, Leukemia and organ transplants) in referred hospitals from central parts of Iran including Isfahan, Markazi, Yazd and Chaharmahale Bakhtiari provinces. Stool samples were analyzed for Cryptosporidium species, modified Ziehl–Neelsen staining techniques followed by the semi-nested PCR and DNA sequencing methods. Results: The total rate of Cryptosporidium spp. was 3.46% (12/346) in the patients, however, the prevalence of the parasite, was 4.6% (4/87) in HIV+/AIDS patients, 3.6% (6/168) in patients with blood malignancy and 2.1% (2/91) in organ transplant recipients. The SSU rRNA gene of Cryptosporidium spp. in all microscopic-positive samples was effectively amplified by the semi-nested PCR and DNA sequences, exposed the existence of two Cryptosporidium species, including C. hominis 91.6% (11/12) and C. parvum 8.3% (1/12). Conclusion: The predominance of C. hominis in the present study may be certifies the importance of anthroponotic transmission of cryptosporidiosis in center of Iran.

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Published
2020-03-09
How to Cite
1.
IZADI S, Ali MOHAGHEGH M, GHAYOUR-NAJAFABADI Z, AZAMI M, MIRZAEI F, NAMDAR F, MOHEBALI M, LESHAN WANNIGAMA D, HEJAZI SH. Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hos-pitals, Central Iran, 2015-16. Iran J Parasitol. 15(1):31-39.
Section
Original Article(s)