Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran
-
M. Rahdar
,
M. Niyyati
,
M. Salehi
,
M. Feghhi
,
M. Makvandi
,
M. Pourmehdi
,
S. Farnia
Abstract
Background: Acanthamoeba spp. are free-living amoebae commonly found in the environmental sources such as water, soil, and air. This ubiquitous amoeba is the causative agent of amoebic kerati- tis (AK). The objective of the present study was to investigate the presence of Acanthamoeba spp. in water and soil sources in Ahvaz City, Khuzestan Province, southern Iran.
Methods: In general, 110 samples of water and soil were taken from different localities of Ahvaz including agricultural canals, rivers, and swimming pools. Filtration and cultivation were carried out on non-nutrient agar medium (NNA). Axenic cultivation was performed for all of positive isolates. PCR analysis was conducted on positive samples. Sequencing was done for 15 PCR products. Geno - types were identified by Blast search and homology analysis.
Result: Acanthamoeba spp. was found in 43 (71.6%) of samples of water and 13 (26%) soil samples. Genotyping of 15 samples proved that Acanthamoeba belonged to T4 (86.6%), T2 (6.6%), and T5 (6.6%) genotypes.
Conclusion: TYI-S-33 medium could be better than PYG medium for Acanthamoeba axenic culture.
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| Issue | Vol 7 No 4 (2012) | |
| Section | Original Article(s) | |
| Keywords | ||
| Acanthamoeba spp. Water Soil TYI-S-33 Iran | ||
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