Short Communication

Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran

Abstract

Background: Dogs, as the definitive host of Neospora caninum, are important in the epidemiology of this parasitic infection. We aimed to determine the prevalence of N. caninum infection in a dog population from a rural setting in Fars Province, Southern Iran, using a combination of molecular and serological techniques.

Methods: This cross-sectional study was carried out in Nov 2018 in three rural districts, Sar Mashhad, HosseinAbad, and Tolesaman located in Kazeroun Township in Fars province, southern Iran. Blood samples were taken from 60 stray and household dogs. Dogs’ sera were tested for antibodies against N. caninum, using a Neospora-Modified Agglutination Test. Moreover, dogs’ buffy coats were tested for Neospora DNA, using a molecular method.

Results: Anti-Neospora antibodies were detected in sera of 4 out of 60 dogs, corresponding to a seroprevalence rate of 6.7%. Out of 25 female dogs, 1 was seropositive and of 35 males, 3 were seropositive, yet the differences were not statistically significant.  The infection was more prevalent in adult dogs (> 12 months), nevertheless, the differences between age and Neospora seropositivity was not statistically significant.  N. caninum DNA was not detected in the buffy coat of any of the studied dogs. 

Conclusion: Findings of the study indicate that N. caninum is a common infection in dogs in rural areas of Fars province in southern Iran. The infected dogs might be a potentially important source of N. caninum infection to livestock in the area.

1. Hosseininejad M, Mahzounieh M, Shams Esfandabadi N. Neospora caninum suspects as one of the most important causes of abortion in large dairy farms in Isfahan, Iran. Iran J Parasitol. 2017;12(3):408-12.
2. Ribeiro CM, Soares IR, Mendes RG, et al. Meta-analysis of the prevalence and risk factors associated with bovine neosporosis. Trop Anim Health Prod. 2019;51(7):1783-1800.
3. Reichel M, Ellis J, Dubey J. Neosporosis and hammondiosis in dogs. J Small Anim Pract. 2007;48(6):308-12.
4. Haddadzadeh HR, Sadrebazzaz A, Malmasi A, et al. Seroprevalence of Neospora caninum infection in dogs from rural and urban environments in Tehran, Iran. Parasitol Res. 2007;101(6):1563-5.
5. Malmasi A, Hosseininejad M, Haddadzadeh H, et al. Serologic study of anti-Neospora caninum antibodies in household dogs and dogs living in dairy and beef cattle farms in Tehran, Iran. Parasitol Res. 2007;100(5):1143-5.
6. Sharifdini M, Mohebali M, Keshavarz H, et al. Neospora caninum and Leishmania infantum co-infection in domestic dogs (Canis familiaris) in Meshkin-Shahr district, Northwestern Iran. Iran J Arthropod Borne Dis. 2011;5(2):60-8.
7. Yakhchali M, Javadi S, Morshedi A. Prevalence of antibodies to Neospora caninum in stray dogs of Urmia, Iran. Parasitol Res. 2010;106(6):1455-8.
8. Barber JS, van Ham L, Polis I, et al. Seroprevalence of antibodies to Neospora caninum in Belgian dogs. J Small Anim Pract. 1997;38(1):15-6.
9. Gao X, Wang H. Seroprevalence and risk factors for Neospora caninum infection in dogs in rural northeastern mainland China. Parasite. 2019;26:32.
10. Dubey J. Neosporosis in cattle. J Parasitol. 2003;89:S42-S56.
11. Dubey J, Schares G. Neosporosis in animals—the last five years. Vet Parasitol. 2011;180(1-2):90-108.
12. Shaapan RM. The common zoonotic protozoal diseases causing abortion. J Parasit Dis. 2016;40(4):1116-29.
13. Packham AE, Sverlow KW, Conrad PA, et al. A modified agglutination test for Neospora caninum: development, optimization, and comparison to the indirect fluorescent-antibody test and enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol. 1998;5(4):467-73.
14. Tavanaee H, Namavari M. Evaluation of attenuated variety of Neospora caninum for diagnosis of infection in cattle by agglutination test. Vet Res Biol Prod. 2017; 30(2):153-157.
15. Abdoli A, Arbabi M, Pirestani M, et al. Molecular assessment of Neospora caninum and Toxoplasma gondii in hooded crows (Corvus cornix) in Tehran, Iran. Comp Immunol Microbiol Infect Dis. 2018;57:69-73.
16. Dijkstra T, Barkema H, Eysker M, et al. Natural transmission routes of Neospora caninum between farm dogs and cattle. Vet Parasitol. 2002;105(2):99-104.
17. Mansourian M, Namavari M, Khodakaram-Tafti A, et al. Experimental Neospora caninum infection in domestic bird's embryonated eggs. J Parasit Dis. 2015;39(2):241-4.
18. Collantes-Fernandez E, Gomez-Bautista M, Miro G, et al. Seroprevalence and risk factors associated with Neospora caninum infection in different dog populations in Spain. Vet Parasitol. 2008;152(1-2):148-51.
19. King JS, Brown GK, Jenkins DJ, et al. Oocysts and high seroprevalence of Neospora caninum in dogs living in remote Aboriginal communities and wild dogs in Australia. Vet Parasitol. 2012;187(1-2):85-92.
20. Pouramini A, Jamshidi S, Shayan P, et al. Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. Iran J Vet Med. 2017;11(2):105-12.
Files
IssueVol 16 No 1 (2021) QRcode
SectionShort Communication(s)
Published2021-02-17
DOI https://doi.org/10.18502/ijpa.v16i1.5534
Keywords
Seroprevalence Neospora caninum Dogs Iran

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
HARIRI M, AREFKHAH N, GHORBANI F, NAMAVARI M, OMIDIAN M, SARKARI B. Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran. Iran J Parasitol. 16(1):146-150.