Molecular Characterization of Echinococcus granulosus from Hydatid Cysts Isolated from Human and Animals in Golestan Province, North of Iran
-
Sh. Gholami
,
M. Sosari
,
M. Fakhar
,
M. Sharif
,
A. Daryani
,
MB. Hashemi
,
M. Vahadi
Abstract
Background: The aim of the present study was to determine the molecular characteristics of Echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using PCR- RFLP of ITS1- rDNA analysis in Golestan Province, northern Iran.
Methods: E. granulosus isolates from human patients infected with hydatid cyst and protoscoleces from hydatid cysts of sheep, cattle and camel isolates were collected from different hospitals and the abattoir throughout the Golestan Province. In all, 60 E. granulosus genomic DNA were extracted and examined by PCR - ITS1 of rDNA and amplified using BD1 / 4S and EGF1 / EGR2 primers, fol- lowed by RFLP using Alu1, Msp1 and TaqI restriction enzymes.
Results: The PCR-ITS1 products obtained from sheep, cattle and human isolates were similar to sheep strain (1000 bp and 391 bp). Majority of the camel samples yielded 295 bp DNA bands. RFLP-ITS1 of E. granulosus with Taq1 in human, sheep and cattle isolates showed similar patterns in the number and size of DNA. RFLP methods in camel isolates showed a different genotype, using Taq1, whereas no DNA bands were observed using Alu1 in camel and human isolates. Therefore, two clearly distinguishable banding patterns of E. granulosus were obtained with the three enzymes, which separating human, sheep and cattle isolates from the camel origin.
Conclusion: The results indicate the possible of transmission of the G1 and G6 genotypes of E. granulosus between livestock animals and human in Golestan Province.
Thompson RCA and McManus DP. Ateiology: Parasite and life-cycles. In: WHO/OIE Manual on Echinococcosis in humans and animals: A Public Health Problem of Global concern. (Eds. J. Eckert, M.A. Gemmell, F.X. Meslin and Z.S. Pawlowski), pp. 1-17. World Health Organization. Animal Health, Geneva; 2001.
Jenkins DJ, Roming T, Thompson RCA. Emergence / re-emergence of Echinococcus SPP. Aglobal update. Int J Parasitol. 2005; 35: 1205-1219.
Thompson RCA and Lymbery AJ. The nature, extent and significance of variation within the genus Echinococcus. Adv Parasitol.1988; 27: 209-258.
Thompson RCA. Biology and systematic of Echinococcus. In Echinococcus and hydatid disease (Eds. R. C. A.Thompson and A. J. Lymbery) pp.1-50. CAB International, Wallingford, UK; 1995.
Thompson RCA and McManus DP. Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002; 18: 452-457.
McManus DP and Thompson RCA. Molecular epidemiology of cystic Echinococcosis. Parasitology. 2003; 127 (Suppl.1): S37-S51.
Thompson RCA. The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol. 2008; 119: 439-446.
Bowles J, Blair D, McManus DP. Molecular genetic characterization of the Cervid strain (northern form) of Echinococcus granulosus. Parasitology. 1994; 109: 215-221.
Scott JC and McManus DP. The random amplification of polymorphic DNA can discriminate species and strains of Echinococcus. Trop Med Parasitol.1994; 45: 1–4.
Scott JC, Stafaniak J, Pawowski ZS, McManus DP. Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9) of Echinococcus granulosus. Parasitology. 1997; 114: 37-43.
Lavikainen A, Lehtinen MJ, Meri T, Hirvela- KoskiV, Meri S. Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology. 2003; 127: 207-215.
Thompson RCA, Boxelli bJ, Raslston CC, Constantine CC, Hobbs RP, Shury, T, Olson ME. Molecular and morphological characterization of Echinococcus in Cervids from North America. Parasitology. 2006; 132: 439-447.
Nakao M, McManus DP, Schantz PM, Craig PS, Ito A. A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology. 2007; 134: 713-722.
Lavikainen A, Lehtinen MJ, Laaksonen S, Agren E, Okasanen A, Meri S. Molecular characterization of Echinococcus isolates of Cervid origin from Finland and Sweden. Parasitology. 2006; 133: 565.
Thompson RCA, Lymbery AJ, Constantine CC. Variation in Echinococcus: Towards a taxonomic revision of the genus. Adv Parasitol. 1995; 35: 145-176.
Bowles J, Blair D, McManus DP. A molecular phylogeny of the genus Echinococcus. Parasitology. 1995; 110: 317-328.
Rosenzvit MC, Zhang LH, Kamenetzky L, Canova SG, Guarnera EA, McManus DP. Genetic variation and epidemiology of Echinococcus granulosus in Argentina. Parasitology. 1999; 118: 523-530.
McManus DP. The molecular epidemiology of Echinococcus granulosus and cystic hydatid disease. Trans R Soc Trop Med Hyg. 2002; 96: S151-S157.
Schneider R, Gollackner B, Edel B, Schmid K, Wrba F, Tucek G, Walochnik J, Auer H. Development of a new PCR protocol for the detection of species and genotypes (strains) of Echinococcus in formalin-fixed, paraffin-embedded tissues. Int J Parasitol 2008; 38: 1065–1071.
Dalimi A, Motamedi G, Hosseini M, Mohammadian B, Malaki H, Ghamari Z, Ghaffari F. Echinococcosis/hydatidosis in western Iran. Vet Parasitol. 2002, 105(2):161-71.
Rokni MB. Echinococcosis/hydatidosis in Iran. Iranian J Parasitol. 2009; 4(2): 1-16.
Fasihi Harandi M, Hobbs RP, Adams PJ, Mobedi I, Morgan-Ryan UM, Thompson RCA. Molecular and morphological characterizationn of Echinococcus granulosus of human and animal origin in Iran. Parasitology. 2002; 125: 367-373.
Kia EB, Rahimi H, Sharbatkhori M, Talebi A, Fasihi Harandi M, Mirhendi H. Genotype identification of human cystic echinococcosis in Isfahan, central Iran. Parasitol Res. 2010; 107:757–760.
Shahnazi M, Hejaz, H, Salehi M, Andalib AR. Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Tropica. 2011; 117:47-50
Mehrabani D, Oryan A, SadjjadiS M. Prevalence of Echinococcus granulosus infection in stray dogs and herbivores in Shiraz, Iran. Vet Parasitol. 1999; 86: 217- 20.
Arbabi M, Hooshyar H. Survey of echinococcosis and hydatidosis in Kahan Region, Central Iran. Iranian J Publ Health. 2006; 35(1): 75-81.
Daryani A, Alaei R, Arab R, Sharif M, Dehghan MH, Ziaei H. The prevalence, intensity and viability of hydatid cysts in slaughtered animals in the Ardabil province of Northwest Iran. J Helminthol. 2007; 81:13-17.
Amin Pour A, Hosseini SH, Shayan P. Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene. Parasitol Res. 2011; 108:1229–1234.
Zhang L, Eslami A, Hosseini SH, McManus DP. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. Am J Trop Med Hyg. 1998; 59: 171-174.
Sharbatkhori M, Fasihi Harandi M, Mirhendi H, Hajialilo E, Kia EB. Sequence analysis of cox1 and nad1 genes in E. granulosus G3 genotype in camels (Camelus dormedarius) from central Iran. Parasitol Res. 2011; 108:521-527.
Jamali R, Ghazanchaei A, Asgharzadeh M. Identification and characterization of Echinococcus granulosus by PCR-RFLP technique in Tabriz district. J Parasitic Dis. 2004; 28(2): 69-72.
Ahmadi N, Dalimi A. Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infection, Genetics and Evolution. 2006; 6: 85-90.
Gholami Sh, Irshadullah M, Asadullah Khan. Genetic variation of Echinococcus granulosus isolates from Indian buffalo and Iranian sheep, cattle and camel. J Mazand Univ Med Sci. 2009; 19(71): 60-69.
Karimi A, Dianatpour R. Genotypic and phenotypic Characterization of Echinococcus granulosus of Iran. Biotechnology. 2008; 7(4):757-762.
Bowles J, McManus DP. Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Mole Biochem Parasitol. 1993; 57: 23.
Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54:165-174.
Rostami Nejad M, Nazemalhosseini Mojarad E, Nochi Z, Fasihi Harandi M, Cheraghipour, K, Mowlavi GR, Zali MR. Echinococcus granulosus strain differentiation in Iran based on sequence heterogeneity in the mitochondrial 12S rRNA gene. J Helminthol. 2008; 82(4): 343-7.
Sharbatkhori M, Mirhendi H, Harandi MF, Rezaeian M, Mohebali M, Eshraghian M, Rahimi H, Kia EB. Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camels. Exp Parasitol. 2010; 124:373– 379.
Sharbatkhori M, Mirhendi H, Jex AR, Pangasa A, Campbell BE, Kia EB, Eshraghian M, Fasihi Harandi M, Gasser RB. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning-phylogenetic approach. Electrophoresis. 2009; 30:2648–2655.
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| Issue | Vol 7 No 4 (2012) | |
| Section | Original Article(s) | |
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| Molecular Characterization Echinococcus granulosus Hydatid cysts Iran | ||
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