Occurrence of Thermotolerant Hartmannella vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran
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Abstract
Background: Geothermal waters could be suitable niches for thermophilic free living amoebae including Naegleria and Hartmannella. Ardebil Province, northwest Iran is popular for having many hot springs for recreational and health purposes activity. The present research is the first molecular based investigation regarding the presence of Naegleria and Hartmannella in the hot springs of Ardebil Province in Iran.
Methods: Overall, 30 water samples were taken from waters of thermal hot springs in Ardebil Province, Iran during 2010-2011. All collected samples were transferred to Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Cultivation of concentrated water samples was performed using culture-enrichment method. Cloning of the target amoebae was obtained and morphological and molecular analysis was done using page key combined with two sets of primers, respectively. Sequence analysis and homology search was used for strains identification.
Results: Of 30 water samples, 8 (26.7%) were positive for thermotolerant Vahlkampfiids and Hartmannella based on morphological characteristics of vegetative form and double walled cysts. Cloning of the target amoebae were done successfully. Sequencing of the positive isolates revealed that the strains belonged to Naegleria (N. carteri and N. spp) and H. vermiformis.
Conclusion: The result highlights a need for improved filtration and disinfection and periodic monitoring of recreational thermal waters in order to prevent disease related to free- living amoebae. This is the first comprehensive molecular study of thermophilic Naegleria and Hartmannella in hot springs of Iran.
Visvesvara GS, Moura H, Schuster FL. Pathogenic and opportunistic free living amoebae: Acanthamoeba spp, Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol Med Microbiol. 2007; 50(1):1-26.
Khan NA. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev. 2006; 30(4): 564–595.
Rezaeian M, Niyyati M. Pathogenic Free Living Amebas In Human. 1st ed. Tehran: TUMS Publication; 2010.
Niyyati M, Lorenzo-Morales J, Rezaie S, Rahimi F, Martín-Navarro CM, Mohebali M, Maghsood AH, Farnia S, Valladares B, Rezaeian M. First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran. Exp Parasitol. 2010; 126(1): 89-90.
Visvesvara GS, Sriram R, Qvarnstorm Y, Bandyopadhyay K, Silva A, Pina NJ, Cabral GA. Paravahlkampfia francinae n. sp. Masquerading as an agent of primary amoebic meningoencephalitis. J Eukaryot Microbiol. 2009; 56: 357–366.
Marciano-Cabral F, Jamerson M, Kaneshiro ES. Free-living amoebae, Legionella and Mycobacterium in tap water supplied by a municipal drinking water utility in the USA. J Water Health. 2010; 8(1): 71-82.
Jamerson, M., Remmers, K., Cabral, G. & Marciano-Cabral, F. Survey for the presence of Naegleria fowleri amebae in lake water used to cool reactors at a nuclear power generating plant. Parasitol Res. 2009 ; 104: 969–978.
De Jonckheere JF. Molecular definition and the ubiquity of species in the genus Naegleria. Protist. 2004; 155: 89–103.
Badirzadeh A, Niyyati M, Babaei Z, Amini H, Badirzadeh H, Rezaeian M. Isolation of Free-Living Amoebae from Sarein Hot Springs in Ardebil Province, Iran. Iranian J Parasitol. 2011; 6 (2):1-8.
Lorenzo-Morales J, Martínez-Carretero E, Batista N, Alvarez-Marín J, Bahaya Y, Walochnik J, Valladares B. Early diagnosis ofamoebic keratitis due to a mixed infection with Acanthamoeba and Hartmannella. Parasitol Res. 2007 ;102(1):167-9.
Page FC (1988). A New Key to Freshwater and Soil Gymnamoebae. Freshwater Biological Association, Ambleside, UK.
Niyyati M, Lorenzo-Morales J, Rezaie S, Rahimi F, Mohebali M, Maghsood AH, Motevalli-haghi A, Martín-Navarro CM, Farnia Sh, Valladares B, Rezaeian M. Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran. Exp Parasitol. 2009; 121(3): 242-245.
Pelandakis M, Pernin P. Use of multiplex PCR and PCR restriction enzyme analysis for detection and exploration of the variability in the free-living amoeba Naegleria in the environment. Appl Environ Microbiol. 2002 ;68: 2061–2065.
Lasjerdi Z, Niyyati M, Haghighi A, Shahabi S, Biderouni FT, Taghipour N, Eftekhar M, Nazemalhosseini Mojarad E. Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran. Parasitol Res. 2011;109(3):575-80.
Kennedy SM, Devine P, Hurley C, Ooi YS, Collum LMT. Corneal infection with Hartmannella vermiformis in contact-lens wearer. Lancet. 1995; 346: 637–638.
Centeno M, Rivera F, Cerva L, Tsutsumi V, Gallegos E, Calderon A, Ortiz R, Bonilla P, Ramirez E, Suarez G. Hartmannella vermiformis isolated from the cerebrospinal fluid of a young male patient with meningoencephalitis and bronchopneumonia. Arch Med Res. 1996; 27:579–586.
Huang SW, Hsu BM. Survey of Naegleria and its resisting bacteria-Legionella in hot spring water of Taiwan using molecular method. Parasitol Res. 2010; 106: 1395-1402.
Huang SW, Hsu BM, Chen NH, Huang CC, Huang KH, Chen JS, Kao PM. Isolation and identification of Legionella and their host amoebae from weak alkaline carbonate spring water using a culture method combined with PCR. Parasitol Res. 2011; DOI: 10.1007/s00436-011-2366-8.
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| Issue | Vol 7 No 2 (2012) | |
| Section | Articles | |
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| Hot springs Hartmannella Naegleria Iran | ||
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