Original Article

Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle


Background: Sarcocystis is a genus of coccidian protozoa that at least seven species of it can parasitize cattle. The global prevalence of Sarcocystis is close to 100% in adult cattle. The main aim of this study was to identify the infection rate of Sarcocystis spp. in heart of cattle in Tehran, Iran by microscopy and PCR-RFLP methods.

Methods: Totally, 100 bovine heart samples were collected from the main slaughterhouse of Shahriar, Meysam slaughterhouse, west of Tehran in 2016. At first, heart samples were completely examined for the presence of sarcocystic macrocysts. Then, for microscopic examination, 50 g of each heart was digested in sterile condition using pepsin acid digestion method. Then, the species of the parasite were detected by PCR-RFLP technique and sequencing.

Results: Overall, 97 of 100 of the heart muscle samples were infected with Sarcocystis. All the samples were detected as S. cruzi through similar patterns in PCR-RFLP.

Conclusion: S. cruzi is the most common species in the heart of cattle slaughtered in Shahriar.

Frenkel J, Smith D. Determination of the genera of cyst-forming coccidia. Parasitol Res. 2003;91(5):384-9.

Dubey J, Lindsay DS. Neosporosis, toxoplasmosis, and sarcocystosis in ruminants. Vet Clin North Am Food Anim Pract. 2006;22(3):645-71.

Dubey J, Calero-Bernal R, Rosenthal B, Speer C, Fayer R. Sarcocystosis of animals and humans: CRC Press; 2015.

Vangeel L, Houf K, Geldhof P, De Preter K, Vercruysse J, Ducatelle R, et al. Different Sarcocystis spp. are present in bovine eosinophilic myositis. Vet Parasitol. 2013;197(3):543-8.

Beaver P, Gadgil R, Morera P. Sarcocystis in man: a review and report of five cases.

Am J Trop Med Hyg. 1979;28(5):819-44.

Fayer R. Epidemiology of protozoan infections: the coccidia. Vet Parasitol. 1980;6(1-3):75-103.

Dubey Jt. A review of Sarcocystis of domestic animals and of other coccidia of cats and dogs. J Am Vet Med Assoc. 1976;169(10):1061-78.

Hajimohammadi B, Eslami G, Zohourtabar A, Dehghani A, Oryan A, Pourmirzaei Tafti H, et al. High occurrence of Sarcocystis cysts in meat produced in Yazd, Central Iran. J Food Qual Hazards Control. 2014;1:95-101.

Hamidinejat H, Jalali MHR, Gharibi D, Molayan PH. Detection of Sarcocystis spp. in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) in Iran by PCR–RFLP. J Parasit Dis. 2015;39(4):658-62.

Dubey JP, Speer C, Fayer R. Sarcocystosis of animals and man: CRC Press, Inc.; 1989.

Gjerde B. Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis). Parasitol Res. 2016;115(4):1473-92.

Dubey JP, van Wilpe E, Calero-Bernal R, Verma SK, Fayer R. Sarcocystis heydorni, n. sp.(Apicomplexa: Sarcocystidae) with cattle (Bos taurus) and human (Homo sapiens) cycle. Parasitol Res. 2015;114(11):4143-7.

Vangeel L, Houf K, Chiers K, Vercruysse J, D'HERDE K, Ducatelle R. Molecular-based identification of Sarcocystis hominis in Belgian minced beef. J Food Prot. 2007;70(6):1523-6.

Moré G, Abrahamovich P, Jurado S, Bacigalupe D, Marin J, Rambeaud M, et al. Prevalence of Sarcocystis spp. in Argentinean cattle. Vet Parasitol. 2011;177(1):162-5.

Pritt B, Trainer T, Simmons-Arnold L, Evans M, Dunams D, Rosenthal BM. Detection of Sarcocystis parasites in retail beef: a regional survey combining histological and genetic detection methods. J Food Prot. 2008;71(10):2144-7.

Fayer R. Sarcocystis spp. in human infections. Clin Microbiol Rev. 2004;17(4):894-902.

Fard SRN, Asghari M, Nouri F. Survey of Sarcocystis infection in slaughtered cattle in Kerman, Iran. Trop Anim Health Prod. 2009;41(8):1633.

Yang Z-Q, Li Q-Q, Zuo Y-X, Chen X-W, Chen Y-J, Nie L, et al. Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: a cost-effective and simple technique for routine species identification. Exp Parasitol. 2002;102(3):212-7.

Xiang Z, Chen X, Yang L, He Y, Jiang R, Rosenthal BM, et al. Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts. Parasitol Int. 2009;58(3):293-6.

Dubey JP. Toxoplasmosis of animals and humans: CRC press; 2016.

Bräunig P, Portella LP, Cezar AS, Libardoni F, Sangioni LA, Vogel FSF, et al. DNA extraction methods and multiple sampling to improve molecular diagnosis of Sarcocystis spp. in cattle hearts. Parasitol Res. 2016;115(10):3913-21.

Moré G, Basso W, Bacigalupe D, Venturini M, Venturini L. Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle. Parasitol Res. 2008;102(4):671-5.

Silva NRSd, Rodrigues RJD, Araujo FAPd, Beck C, Olicheski AT. Detection of bovine Sarcocystis cruzi cysts in cardiac muscles: a new technique of concentration for diagnostic. Acta Sci Vet, RS Vol 30, n 2 (2002), p 127-129. 2002.

Nourollahi-Fard SR, Kheirandish R, Sattari S. Prevalence and histopathological finding of thin-walled and thick-walled Sarcocysts in slaughtered cattle of Karaj abattoir, Iran. Acta Sci Vet. 2015;39(2):272-5.

Hajimohammadi B, Eslami G, Zohourtabar A, Dehghani A, Oryan A, Pourmirzaei Tafti H, et al. High occurrence of Sarcocystis cysts in meat produced in Yazd, Central Iran. J Food Qual Hazards Control. 2014;1(4):95-101.

Hornok S, Mester A, Takács N, Baska F, Majoros G, Fok É, et al. Sarcocystis-infection of cattle in Hungary. Parasit Vectors. 2015;8(1):69.

Jehle C, Dinkel A, Sander A, Morent M, Romig T, Luc P, et al. Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam. Vet Parasitol. 2009;166(3):314-20.

Obijiaku IN, Ajogi I, Umoh JU, Lawal IA, Atu BO. Sarcocystis infection in slaughtered cattle in Zango abattoir, Zaria, Nigeria. Vet World. 2013;6(6):346-9.

Taib M, Harhoura K, Aissi M, Chaouadi M, Djouhri Y. Study of the bovine Sarcosporidiosis in the slaughterhouses of the North of Algeria: Case of the Slaughterhouses of El Harrach (Algiers). Cell Dev Biol. 2016;5:167.

Hamidinejat H, Jalali MHR, Nabavi L. Survey on sarcocystis infection in slaughtered cattle in South-West of Iran, emphasized on evaluation of muscle squash in comparison with digestion method. J. Animal Veterinary Adv. 2010;9(12):1724-6.

Arshad M, Dalimi A, Ghaffarifar F. Comparative study on Sarcocystis diagnosis in meat of slaughtered sheep in Tabriz. Iranian Vet J. 2008.

Okur H, Kandemir O, Sahine I. An investigation on Sarcocystis species in cattle and sheep from Bayburt. Turkiye Parazitol Derg. 1995;19:113-8.

IssueVol 17 No 4 (2022) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijpa.v17i4.11281
Protozoa Parasite Cysts

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How to Cite
Dalir Ghaffari A, Dalimi A, Pirestani M. Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle. Iran J Parasitol. 2022;17(4):535-542.