Comparative Morphologic and Morphometric Study on the Developmental Aspects of In Vitro and In Vivo Reared Echino-coccus granulosus Sensu Stricto Using Differential Interfer-ence Contrast (DIC)/Nomarski and Phase Contrast Microsco-py

  • Seyedeh Faezeh SADJJADI Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
  • Mina MOTAMEDI Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
  • Tahereh MOHAMMADZADEH Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Department of Parasitology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Seyed Mahmoud SADJJADI Mail Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Keywords:
Echinococcus granulosus sensu stricto, G1 strain, DIC/Nomarski microscopy, Phase contrast, Morphology, Morphometry

Abstract

Background: Echinococcus granulosus is a zoonotic parasite with worldwide distribution. The present study focused on comparative morphologic and morphometric observations on the developmental aspects of whole body, more special the reproductive structures of in vitro reared adult worms (RAW) and in vivo reared adult worms in definitive host (AWIDH) using differential interference contrast (DIC)/Nomarski, phase contrast and routine optical microscopy. Methods: A total number of 10 in vitro and 10 in vivo reared adult worms of E. granulosus sensu stricto, G1 strain were selected. The worms were processed by Formaldehyde-Alcohol-Azocarmine-Lactophenol (FAAL). The details of morphological factors and reproductive structures of each worm including 25 biometrical parameters were studied by routine optical, phase contrast and Nomarski microscopy. The details of the samples were photographed, measured and analyzed. The fine structures of the parasite including the details of cirrus sac and developmental stages in different strobila were more obvious observing by Nomarski microscopy.  Results: The morphometric characters in the RAW and AWIDH showed that length of immature proglottid, length of mature proglottid, length of suckers are larger in RAW than AWIDH worms with statistical difference. Characters in E. granulosus of RAW and AWIDH showed that total number of segments, number of mature segments and the total number of testes were greater in RAW than AWIDH worms; while only the number of mature segments was statistically different is two groups. Conclusion: Application of DIC/Nomarski and phase contrast microscopy together with morphometric criteria are useful means for comparing the developmental aspects of in vitro and in vivo reared adults of E. granulosus.

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Published
2019-12-29
How to Cite
1.
SADJJADI SF, MOTAMEDI M, MOHAMMADZADEH T, SADJJADI SM. Comparative Morphologic and Morphometric Study on the Developmental Aspects of In Vitro and In Vivo Reared Echino-coccus granulosus Sensu Stricto Using Differential Interfer-ence Contrast (DIC)/Nomarski and Phase Contrast Microsco-py. Iran J Parasitol. 14(4):510-520.
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Original Article(s)