Short Communication

In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER)


Background: Due to numerous side effects of common drugs in treatment of leishmaniasis, new therapeutic approaches focus on herbal compounds. Therefore, we aimed to determine the effect of crocin and stigmasterol on in-vitro growth of promastigotes and amastigotes of Leishmania major in the Department of Parasitology, Pasteur Institute, Tehran, Iran in 2018.

Methods: The effect of different concentrations of crocin and stigmasterol were evaluated by determining their in-vitro inhibitory effects on promastigotes and amastigotes of the L. major using MTT assay.

Results: The fatality rate was 65.27% and 71.96% for crocin and stigmasterol respectively at 24 h post-culture in concentration of 50 μg/mL. The mean inhibitory effect of crocin and stigmasterol on L major amastigotes after 72 h were 52.22% and 38.96%.

Conclusion: The crocin and stigmasterol had efficient adverse effects on promastigote and amastigotes of L. major, hence, further studies on the anti-leishmanial effects of these herbal compounds in human and animal models are recommended.

Reithinger, R., et al., Cutaneous leishmaniasis. The Lancet infectious diseases, 2007. 7(9): p. 581-596.

Schwartz, E., C. Hatz, and J. Blum, New world cutaneous leishmaniasis in travellers. The Lancet infectious diseases, 2006. 6(6): p. 342-349.

Alvar, J., et al., Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 7(5): p. e35671.

Karami, M., M. Doudi, and M. Setorki, Assessing epidemiology of cutaneous leishmaniasis in Isfahan, Iran. Journal of vector borne diseases. 50(1): p. 30.

Farahmand, M., et al., An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran. The Brazilian Journal of Infectious Diseases. 15(1): p. 17-21.

Pourmohammadi, B., et al., Glucantime efficacy in the treatment of zoonotic cutaneous leishmaniasis. Southeast Asian Journal of Tropical Medicine and Public Health: p. 502-508.

Sadeghian, G., et al., Decreased effect of glucantime in cutaneous leishmaniasis complicated with secondary bacterial infection. Indian journal of dermatology. 56(1): p. 37.

Rezaee, R., et al., Cytotoxic effects of crocin on MOLT-4 human leukemia cells. Journal of Complementary and Integrative Medicine. 10(1): p. 105-112.

Kaur, N., et al., Stigmasterol: a comprehensive review. International Journal of Pharmaceutical Sciences and Research. 2(9): p. 2259.

Duque, G.A. and A. Descoteaux, Macrophage cytokines: involvement in immunity and infectious diseases. Frontiers in immunology. 5.

Liu, D. and J.E. Uzonna, The early interaction of Leishmania with macrophages and dendritic cells and its influence on the host immune response. Frontiers in cellular and infection microbiology. 2.

Mehravaran, A., et al., The role of ISCOMATRIX bilayer composition to induce a cell mediated immunity and protection against leishmaniasis in BALB/c mice. Iranian journal of basic medical sciences. 19(2): p. 178.

Ghaffarifar, F., Leishmania major: in vitro and in vivo anti-leishmanial effect of cantharidin. Experimental parasitology. 126(2): p. 126-129.

Khademvatan, S., et al., Miltefosine-induced apoptotic cell death on Leishmania major and L. tropica strains. The Korean journal of parasitology. 49(1): p. 17.

Maroufi, Y., et al., A study on the cytotoxic effect of cantharidin on Leishmania major promastigote and amastigote survival in vitro. Feyz Journals of Kashan University of Medical Sciences. 16(5).

Kaye, P. and P. Scott, Leishmaniasis: complexity at the host–pathogen interface. Nature Reviews Microbiology. 9(8): p. 604-615.

Monge-Maillo, B.a. and R. Lأ³pez-Vأ©lez, Therapeutic options for old world cutaneous leishmaniasis and new world cutaneous and mucocutaneous leishmaniasis. Drugs. 73(17): p. 1889-1920.

De Monte, C., et al., Bioactive compounds of Crocus sativus L. and their semi-synthetic derivatives as promising anti-Helicobacter pylori, anti-malarial and anti-leishmanial agents. Journal of enzyme inhibition and medicinal chemistry. 30(6): p. 1027-1033.

Sen, R. and M. Chatterjee, Plant derived therapeutics for the treatment of Leishmaniasis. Phytomedicine. 18(12): p. 1056-1069.

Nylأ©n, S. and S. Gautam, Immunological perspectives of leishmaniasis. Journal of global infectious diseases. 2(2): p. 135.

Huang, C.-H., et al., Prebiotic effect of diosgenin, an immunoactive steroidal sapogenin of the Chinese yam. Food chemistry. 132(1): p. 428-432.

Dar, N.J., A. Hamid, and M. Ahmad, Pharmacologic overview of Withania somnifera, the Indian Ginseng. Cellular and molecular life sciences. 72(23): p. 4445-4460.

Kabeer, F.A., et al., Isodeoxyelephantopin from Elephantopus scaber (Didancao) induces cell cycle arrest and caspase-3-mediated apoptosis in breast carcinoma T47D cells and lung carcinoma A549 cells. Chinese medicine. 9(1): p. 14.

Looi, C.Y., Pharmacological Activities and Chemical Constituents of. J. Med. Sci. 13(4): p. 236-243.

Sirisha, N., et al., Antioxidant properties of Ficus species–a review. Int J PharmTech Res. 2(4): p. 2174-2182.

Brasili, E., et al., A non-targeted metabolomics approach to evaluate the effects of biomass growth and chitosan elicitation on primary and secondary metabolism of Hypericum perforatum in vitro roots. Metabolomics. 10(6): p. 0.

أ–zipek, M., et al., Leishmanicidal cycloartane-type triterpene glycosides from Astragalus oleifolius. Phytochemistry, 2005. 66(10): p. 1168-1173.

Cheikh-Ali, Z., et al., Labdane diterpenoids from Aframomum sceptrum: NMR study and antiparasitic activities. Phytochemistry Letters. 4(3): p. 240-244.

Rosenthal, E. and P. Marty, Recent understanding in the treatment of visceral leishmaniasis. Journal of postgraduate medicine, 2003. 49(1): p. 61.

Nakhaei, M., M. Khaje-Karamoddin, and M. Ramezani, Inhibition of Helicobacter pylori growth in vitro by saffron (Crocus sativus L.). Iranian journal of basic medical sciences, 2008. 11(2): p. 91-96.

IssueVol 16 No 1 (2021) QRcode
SectionShort Communication(s)
Crocin Tigmasterol Leishmania major Promastigote Amastigote In vitro

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How to Cite
RANJBAR R, SHAYANFAR P, MANIATI M. In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER). Iran J Parasitol. 2021;16(1):151-158.