Novel in Vitro Efficiency of Chitosan Biomolecule against Trichomonas gallinae

  • M Tavassoli Mail Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
  • A Imani Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
  • H Tajik Department of Food Science and Technology, Ahar Faculty of Agriculture, University of Tabriz, Tabriz, Iran
  • M Moradi Department of Food Science and Technology, Ahar Faculty of Agriculture, University of Tabriz, Tabriz, Iran
  • SH Pourseyed Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Keywords:
Trichomonas gallinae, Chitosan, Antiparasitic drugs, Trophozoite, In Vitro

Abstract

Background: Development of new natural agents for parasitic diseases treatment has unexpec­tedly increased to overcome effectively against emergence and re-emergence of parasitic diseases, the appearance of drug resistant organisms and toxic side effects of current agents. The aim of the study was to evaluate antiprotozoal activities of chitosan biomolecule on trophozoites of Trichomo­nas gallinae.

Methods: The antitrichomonal activity of various low molecular weight chitosan concentrations including 125, 250, 500 and 1250 μg ml-1 against T. gallinae trophozoites cultured in trypticase-yeast extract-maltose medium supplemented with heat-inactivated cold horse serum was eva­luated in vitro. Samples containing medium without chitosan were also assayed as controls.

Results: The mortality rates at 0, 3 and 6 h post treatment with all concentrations were signifi­cantly different from control group (P<0.05). Treated trophozoites showed more susceptibility to the highest concentration reaching mortality rate of 100% at 3h post inoculation. However, at this time, results for 125, 250 and 500 μg ml-1 were 93%, 95% and 96.7%, respectively.

Conclusion: The results demonstrate that the application of chitosan biomolecule is a promising option for treatment of trichomoniasis in pigeons.

References

Anthony JP, Fyfe L, Smith H. Plant active components – a resource for antiparasitic agents? Trends Parasitol. 2005; 21: 462- 68.

Rahuman AA, Bagavan A, Kamaraj C, Saravanan E, Zahir AA, Elango G. Efficacy of larvicidal botanical extracts against Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res. 2009; 104: 1365-72.

Tariq KA, Chishti MZ, Ahmad F, Shawl AS. Anthelmintic activity of extracts of Artemisia absinthium against ovine nematodes. Vet Parasitol. 2009; 160: 83–88.

Mehlhorn H, Al-Quraishy S, Aziza A, Hess M. Fine structure of the bird parasites Trichomonas gallinae and Tetratrichomonas gallinarum from cultures. Parasitol Res. 2009; 105 (3): 751-56.

Friend M, Franson JC. Field manual of wildlife diseases: general field procedures and diseases of birds. USGS Biological Resources Division Madison. USA.1999.

MoradiM, Tajik H, No HK, RazaviRohani SM, Oromiehie A, Ghasemi S. Potential inherent properties of chitosan and its applications in preserving muscle food. J Chitin Chitosan. 2010; 15(1): 35-45.

Shahidi F, Abuzaytoun R. Chitin, Chitosan, and co-products: Chemistry, Production, Application, and Health effects. Adv Food Nutr Res. 2005; 49: 93-135.

Speiciene V, Guilmineau F, Kulozik U, Leskauskaite

D. The effect of chitosan on the properties of emulsions stabilized by whey proteins. Food Chem. 2006; 102: 1048-54.

Issa MM, Köping-Höggård M, Artursson P. Chitosan and the mucosal delivery of biotechnology drugs. Drug Discov. Today Technol. 2005; 2(1): 1-6.

Brown TJ, Emelko MB. Chitosan and metal salt coagulant impacts on Cryptosporidium and microsphere removal by filtration. Water Res. 2008; 43(2): 331-38.

Parama A, Luzardo A, Blanco-Méndez J, Sanmartín ML, Leiro J. In vitro efficacy of glutaraldehyde-crosslinked chitosan microspheres against the fish-pathogenic ciliate Philasterides dicentrarchi . Dis Aquat Organ. 2005; 64: 151-158.

Munaz E, Castella J, Gutierrez JF. In vivo and in vitro sensitivity of Trichomonas gallinae to some nitroimidazole drugs. Vet Parasitol. 1998; 78: 239-46.

Oyedapo AO, Makanju VO, Adewunmi CO, Iwalewa EO, Adenowo TK. Antitrichomonal activity of 1,3-Diaryl-2-Propen-1-Ones on Trichomonas gallinae. Afr J Trad CAM. 2004; 1: 55 – 62.

Diamond LS. Lumen dwelling protozoa: Entamoeba, Trichomonads and Giardia. In: Jensen JB. (ed) In vitro cultivation of protozoan parasites. CRC Press, Florida. 1983. pp. 65–111.

Tajik H, Moradi M, Razavi-Rohani SM, Erfani AM, Sabet –Jalali FS. Preparation of chitosan from brine shrimp (Artemia urmiana) cyst shells and effects of different chemical processing sequences on the physicochemical and functional properties of the

How to Cite
1.
Tavassoli M, Imani A, Tajik H, Moradi M, Pourseyed S. Novel in Vitro Efficiency of Chitosan Biomolecule against Trichomonas gallinae. Iran J Parasitol. 7(1):92-96.
Section
Articles