Oxidant/Antioxidant Status, PON1 and ARES Activities, Trace Element Levels, and Histological Alterations in Sheep with Cystic Echinococcosis
Background: Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI), nitric oxide (NO), zinc (Zn), copper (Cu) levels, paraoxonase (PON1), arylesterase (ARES) activities, and biochemical changes were studied on sheep with cystic echinococcosis.
Methods: The materials were taken from 2-3 yr old sheep slaughtered in Van Province, Turkey in 2017. Before the slaughter, blood samples were collected from the healthy sheep, while various organs of animals were examined for hydatid cysts after the slaughter. Thirty sheep were protoscolex positive, hydatic group, while 30 sheep that did not have any pathological lesions in organ examinations were accepted as the control group. TOS levels, PON1 and ARES activities, and Zn levels were determined by commercial kits, while Cu levels were determined by atomic absorption spectrophotometer. The collected data were then statistically analyzed.
Results: Serum TOS and OSI levels were significantly higher in sheep with cystic echinococcosis compared to the control group (P<0.001). TAS levels (P<0.01), PON1 and ARES activities, on the other hand, were significantly higher in control group compared to the cystic echinococcosis group (P<0.001). There were no significant differences in Zn, NO and Cu levels between the groups.
Conclusion: PON1 and ARES activities increased in sheep infected with cyst hydatid. The decline of antioxidant reserves in the metabolism results in excessive amounts of free radicals, along with alterations of the normal histological structure of the cystic organ and changes in trace element metabolism.
Ersayit D. Oxidative stres in patients with cystic echinococcosis: Relationship between oxidant and antioxidant parameters. Master Thesis University of Erciyes, Kayseri, Turkey, 2009.
Mert N, Güler AH. Kist Hidatid Sıvılarının Biyokimyasal İçeriği III. Elektrolitler, Ulu Vet Fak Derg. 1991; 10: 29-32.
Hira P, Bahr G, Shweiki H, Behbehani K. An enzyme linked immunosorbent assay using an arc 5 antigen for the diagnosis of cystic hydatid diseases. Ann Trop Med Parasitol. 1990; 84 (2): 157-162.
Miller JK, Brzezinska-slebodzinska E, Madsen FC. Oxidative stress, antioxidants, and animal function. J Dairy Sci. 1993; 76 (9): 2812-2823.
Woodbury RG, Miller HRP, Huntley JF, Newlands GFJ, Palliser AC, Wakelin D. Mucosal mast cells are functionally active during spontaneous expulsion of intestinal nematode infections in rat. Nature. 1984; 312 (5993): 450-452.
Özgün E, Özgün GS, Eskiocak S, Yalçın Ö. Gökmen SS. Effect of L-carnitine on serum paraoxonase, arylesterase and lactonase activities and oxidative status in experimental colitis. Turk J Biochem. 2013; 38 (2): 145–153
Tarpey MM, Wink DA, Grisham MB. Methods for detection of reactive metabolites of oxygen and nitrogen: in vitro and in vivo considerations. Am J Physiol Regul Integr Comp Physiol. 2004; 286 (3): 431-444.
Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37 (4): 277-85.
Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38 (12): 1103-11.
Harma M, Harma M, Erel O. Increased oxidative stress in patients with hydatidiform mole. Swiss Med Wkly. 2003; 133(41- 42): 563-566.
Halliwell B. Reactive oxygen species in living systems: source biochemistry, role in human disease. Am J Med. 1991; 91 (3C): 14-22.
Halliwell B, Gutteridege JMC. Free Radicals in Biology and Medicine, fourth ed. Oxford University Press, Grune Strottan, New York; 2007.
Yılmaz M, Mert H, Irak K, Erten R, Mert N. The Effect of Fucoidan on the Gentamicin Induced Nephrotoxicity in Rats. Fresenius Environmental Bulletin. 2018, 27 (4): 2235-41.
Halliwell B. Free radicals antioxidants, human disease: curiosity cause or consequence. Lancet. 1994; 344 (8924): 721-4.
Gülcü F, Gürsu MF. The Standardization of Paraoxonase and Arylesterase Activity Measurements. Turk J Biochem. 2003; 28: 45-49.
Kurban S, Akpınar Z, Mehmetoğlu İ. Investigation of serum paraoxonase and arylesterase activities and oxidative stress in patients with multiple sclerosis. Genel Tıp Derg. 2010; 20 (1): 13-17.
Mayer B, Hemmens B. Biosynthesis and action of nitric oxide in mammalian cells. Trends Biochem Sci. 1997; 22 (12): 477-481.
Kılınç A, Kılınç K Nitrik Oksit: Biyolojik Fonksiyonları ve Toksik Etkileri. Palme Yayıncılık, Ankara; 2003.
Moncada S, Palmer RM, Higgs EA. Nitric oxide physiology, pathophysiology and pharmacology. Pharmacol Rev. 1991; 43 (2): 109-142.
James SL. Role of nitric oxide in parasitic infection. Microb Rev. 1995; 59: 533–547.
Akaike T, Noguchi Y, Ijiri S, Setoguchi K, Suga M, Zheng YM, Dietzschold B,Maeda H. Pathogenesis of influenza virus-induced pneumonia: involvement of both nitric oxide and oxygen radicals. Proc Natl Acad Sci USA. 1996; 93 (6): 2448-2453.
Umezawa K, Akaike T, Fuji S, Suga M, Setoguchi K, Ozawa A, Maeda H. Induction of nitric oxide synthesis and xanthine oxidase and their roles in the antimicrobial mechanism against Salmonella typhimurium infection in mice. Infect Immun. 1997; 65 (7): 2932-40.
Elahi S, Pang G, Ashman RB, Clancy R. Nitric oxide-enhanced resistance to oral candidiasis. Immunology. 2001; 104 (4): 447–454.
Zeghir-Bouteldja R, Amri M, Aitaissa S, Bouaziz S, Mezioug D, Touil-Boukoffa C. In vitro study of nitric oxide metabolites effects on human hydatid of Echinococcus granulosus. J Parasitol Res. 2009: 624919.
Russel L, McDowell J. Vitamin in animal nutrition. San Diego, California, Academic Press Inc. pp: 92-10, 1989.
Derda M, Wandurska-Nowak E, Hadas E. Changes in the level of antioxidants in the blood from mice infected with Trichinella spiralis. Parasitol. Res. 2004; 93 (3): 207–210.
Şimşek S, Yuce A, Utuk AE. Determination of serum malondialdehyde levels in sheep naturally infected with Dicrocoelium dendriticum. F.Ü. Sağlık Bil. Derg. 2006; 20 (3): 217-220.
Saleh MA. Circulating oxidative stress status in desert sheep naturally infected with Fasciola hepatica. Vet Parasitol. 2008; 154 (3-4): 262-269.
Saleh MA, Al-Salahy MB, Sanousi SA. Oxidative stress in blood of camels (Camelus dromedaries) naturally infected with Trypanosoma evansi. Vet Parasitol. 2009; 162 (3-4): 192-199.
Güralp N. Helmintoloji. Ankara Üniv Vet Fak. Yayınları, 2. Baskı, Yay. No: 368, Ankara; 1981.
Umur Ş. Hidatidozun (Kist Hidatik) önemi, korunma yolları ve eradikasyonu için bir öneri. Vet Hekim Dern Derg. 1995; 65 (4): 18-22.
Yazar S, Altıntaş N. Serodiagnosis of cystic echinococcosis in Turkey. Helminthologia. 2003; 40: 9-13.
Amanvermez R. The effectiveness of oxidants (roi, rni) in echinococcus granulosus hydatid cysts and an investigation of their antioxidant systems. Ph.D.Thesis University of Ondokuz Mayıs, Samsun, Turkey, 1997.
Koltaş IS, Yücebilgiç G, Bilgin R, Parsak CK, Sakman G. Serum malondialdehyde level in patients with cystic echinococcosis. Saudi Med J. 2006; 27 (11): 1703-1705.
Ersayit D, Kılıç E, Yazar S Artı T. Oxidative Stres in Patients with Cystic Echninococcosis: Relationship Between Oxidant and Antioxidant Parameters. Erciyes University J. Health Sci. 2009; 18: 159-166.
Kılıç E, Yazar S, Başkol G, Artiş T, Ersayit D. Antioxidant and nitric oxide status in patients diagnosed with Echinococcus granulosus. Afr. J. Microbiol. Res. 2010; 4 (22): 2439-2443.
Heidarpour M, Mohri M, Borji H, Moghdass E. Oxidative stress and trace elements in camel (Camelus dromedarius) with liver cystic echinococcosis. Vet Parasitol. 2012; 187 (3-4): 459-463.
Heidarpour M, Mohri M, Borji H, Moghdass E. Oxidant/ antioxidant balance and trace elements status in sheep with liver cystic echinococcosis. Comp. Clin. Pathol. 2013; 22 (6): 1043–1049.
Heidarpour M, Mohri M, Borji H, Moghdass E. Oxidant/antioxidant status in cattle with liver cystic echinococcosis. Vet Parasitol. 2013; 195 (1-2): 131-135.
Hanedan B, Kırbaş A, Kandemir F M, Özkaraca M, Kılıç K, Benzer F. Arginase activity and total oxidant/antioxidant capacity in cows with lung cystic echinococcosis. Med. Weter. 2015; 71 (3): 167-170.
Bakır S, Çeliksöz A, Çelik VK, Sarı İ, Aydın H. Antioxidant-Oxidant Status in Patients with Hydatid Cyst. Turk Biyokim Derg. 2012; 37 (1): 29-34.
Al-Gubory KH, Fowler PA, Garrel C. The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes.
Int J Biochem Cell Biol. 2010; 42 (10): 1634-1650.
|Issue||Vol 13 No 3 (2018)|
|Arylesterase Cystic echinococcosis Oxidative stress Paraoxonase 1 Sheep Trace elements|
|Rights and permissions|
|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|