Molecular and Seroepidemiological Survey of Visceral Leishmaniasis among Humans and Domestic Dogs in Mazandaran Province, North of Iran
-
M Fakhar
,
B Rahmati
,
S Gohardehi
,
M Mohebali
,
B Akhoundi
,
M Sharif
,
S Ali Mahdavi
Abstract
Background: New cases of visceral leishmaniasis (VL) have been reported recently in some parts of Mazandaran Province, north of Iran where the first human case of VL was reported in 1949. This study aimed to determine the present status of Leishmania infantum infection among humans and domestic dogs using serological and molecular methods in central parts of Mazandaran Province.
Methods: In this cross-sectional study, blood samples were randomly collected from 402 humans and forty-nine domestic dogs throughout 2009 and 2010 in the central part of Mazandaran Province including Semeskadeh and Kiakola districts where recent cases of human visceral leishmaniasis had been reported there. All the collected samples were tested by direct agglutination test (DAT) for the detection of anti-Leishmania infantum antibodies as well as convenience PCR assay on whole blood samples for detection of leishmanial infection and identification of Leishmania species.
Results: None of 402 collected human (402) and dog (49) blood samples showed anti Leishmania infantum antibodies at titers 1:3200 and 1:320 as cut-off values of DAT, respectively but only 2 of domestic dogs (4.1 %) were found PCR-positive corresponding to L .infantum.
Conclusion: This study confirms the circulation of L. infantum at least among domestic dogs and highlights the sporadic pattern of VL in the studied areas. Further investigations regarding to sand flies fauna and wild canines as reservoir hosts of the disease, are recommended.
World Health Organization (2000). Leishmaniasis and Leishmania/HIV Co-infection.Document WHO/CDS/CSR/ISR/2000.1. WHO: Geneva.
Banuls AL, Hide M, Prugnolle F. Advances in Parasitology. Vol 64, 2007 Elsevier Ltd.
World Health Organization ( 2010). First WHO report on neglected tropical diseases: working to overcome the global impact of neglected tropical diseases WHO/HTM/NTD/2010.1,PP:91-96.
Desjeux P. The increase in risk factorsfor leishmaniasis worldwide. Trans Royal Soc Trop Med Hyg. 2001; 95: 239–243.
Caldas AJMBB, Costa J, Aquino D, Silva AAM, Barral-Netto M, Barral A. Are there differences in clinical and laboratory parameters between children and adults with American leishmaniasis? Acta Trop. 2006; 97: 252-258. 6. Mazloumi Gavgani AS, Mohite H, Edrissian GH, Mohebali M, Davies CR. Domestic dog ownership in Iran is a risk factor for human infection with Leishmania. infantum. Am J Trop Med Hyg. 2002; 67: 511-515.
Asgari Q, Fakhar M, Motazedian MH. Nomadic kala-azar in South of Iran. Iranian J Publ Health. 2006; 35: 85-6.
Mohebali M, Hamzavi Y, Edrissian GH, Forouzani A. Seroepidemiological study of visceral leishmaniasis among humans and animal reservoirs in Bushehr Province. Islamic Republic of Iran. East Mediterr Health J. 2001; 7:912–917.
Mohebali M, Hajjaran H, Hamzavi Y, Mobedi I , Arshi Sh , Zarei Z , Akhoundi B , Naeini K , Manouchehri AR, Fakhar M. Epidemiological aspects of canine visceral leishmaniasis in the Islamic Republic of Iran. Vet Parasitol. 2005; 129: 243-259.
Edrissian GH, Nadim A, Alborzi AV, Ardehali S. Visceral leishmaniasis: the Iranian experiences. Arch Iranian Med. 1999; 1: 22-26.
Fakhar M, Mohebali M, Barani M. Identification of endemic focus of Kalaazar and Seroepidemiological study of visceral Leishmania infection in human and canine in Qom Province. Armaghan-Danesh (In Persian). 2004; 9: 43-52.
Fakhar M, Motazedian MH, Asgari Q, Mohebali M, Mehrabani D. A new endemic focus of visceral leishmaniosis in southern Iran. Armaghane-Danesh (In Persian). 2006; 11: 104-110.
Pouya Y. Study of visceral leishmaniasis in Gilan and Mazadndran provinces of Iran. J Med Fac Tehran. 1949; 7: 359-361.
Sawad koohi R. Study of visceral leishmaniasis cases in Mazandaran Province during 1992-1997. Proceeding of 7th Congress of Infectious and Tropical Diseases. 1998;Tehran , Iran.
Rahmati B, Fakhar M, Sawad koohi R, Mahdavi S, Parsaei M.R, Bahrami S , Mohebali M et al. Reemerging of visceral leishmaniasis in Mazandaran Province, North of Iran. Proceeding of 11th Iranian Microbiology Congress and Eastern Mediterranean Microbiology Congress, 10-13 May 2010; Guilan, Iran.
Shirzadi MR, Sharifian J, Zeinali M, editors. Successful in zoonosis control programmes. 1st ed. Tehran, Ministry of Health and Medical Education Publications 2009; pp: 76-104.
Gholami Sh, Shahabi S, Mobedi I. Prevalence of leishmaniasis in rodents’ population in Jangle area of Semeskandeh district from Sari township, Mazandaran Province. Proceeding of 8th Congress of Infectious and Tropical Diseases. 1999; Tehran, Iran
Hamidi AN, Nadim A, Edrissian GH, Tahvildar Bidruni G, Javadian E. Visceral leishmaniasis of jackals and dogs in northern Iran. Trans R Soc TropMed Hyg 1982; 76(6):756-7.
Mahjouri E. Historical Geography of Mazandaran Province. Geographical Organization Press ,Tehran ;2001.
20- Harith A , Salappendel RJ , Reiter I , Knapen F , Korte P , Huigen E , Kolk RHG. Application of a direct agglutination test for detection of specific anti- leishmania antibodies in the canine reservoir. J Clin Microbiol. 1989; 27: 2252-7.
Motazedian H, Karamian M, Noyes HA, Ardehali S. DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR. Ann Trop Med Parasitol. 2002, 96: 31-34.
Fakhar M, Motazedian MH, Hatam GR, Asgar Q, Kalantari M, Mohebali M. Asymptomatic human carriers of Leishmania infantum: possible reservoirs for Mediterranean visceral leishmaniasis in southern Iran. Ann TropMed Parasitol. 2008; 102: 577-83.
MohebaliM, Edrissian GHH, Nadim A, Hajjaran H, Akhoundi B, Hooshmand B, Zarei Z, Arshi S, Mirsamadi N, Manouchehri-Naeini K, Mamishi S, Sanati AA, Moshfe AA, Charehdar S, Fakhar M. Application of direct agglutination test (DAT) for the diagnosis and seroepidemiological studies of visceral leishmaniasis in Iran. Iranian J Parasitol. 2006; 1: 15-25.
Sundar S, Rai M. Laboratory diagnosis of visceral leishmaniasis. Clin Diag Lab Immunol. 2002; 9: 951-958.
Singh S. New developments in diagnosis of Leishmaniasis. Indian. J Med Res. 2006; 123: 311-330.
Riera C, Fisa R, Udina M, Gállego M, Portus M. Detection of Leishmania infantum cryptic infection in asymptomatic blood donors living in an endemic area (Eivissa, Balearic Islands, Spain) by different diagnostic methods. Trans R Soc Trop Med Hyg. 2004; 98:102.
Maurya R, Singh RK, Kumar B, Salotra P, Rai M, Sundar S. Evaluation of PCR for diagnosis of Indian kalaazar and assessment of cure. J Clin Microbiol. 2005; 43: 3038-3041.
Bossolasco S, Gaiera G, Olchini D, Gulletta M, Martello L, Bestetti A. Real-time PCR assay for clinical management of human immunodeficiency virus-infected patients with visceral leishmaniasis. J Clin Microbiol. 2003; 41: 5080-5084.
Gao CH,Wang JY, Yang YT, Bao YF. Study on PCR method for detecting the asymptomatic infection of Leishmania infantum Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006;24(2): 92-96.
Cascio A, Calattini S, Colomba C, Scalamonga C, Galazzi M, Pizzuto M, Camilli R, Gramiccia M, Titone L, Corbellino M, Antinori S. Polymerase chain reaction in the diagnosis and prognosis of Mediterranean visceral leishmaniasis in immunocompetent children. Pediatrics. 2002; 109: 1-5.
Moshfe A, Mohebali M, Edrissian GhH, Zarei Z, Akhoundi B, Kazemi B, Jamshidie Sh, Mahmoodi M. Seroepidemiological study on canine visceral leishmaniasis in Meshkin-Shahr District, Ardabil Province, northwest of Iran during 2006-2007. Iranian J Parasitol. 2008; 3: 1-10.
Ashford DA, David JR, Freire M, David R, Sherlock I, Eulalio MC, Sampanio DP, Badaro R. Studies on control of visceral leishmaniasis impact of dog control on canine and human visceral leishmaniasis in Jacobina, Bahia, Brazil. Am J TropMed Hyge. 1998; 28-59.
Kumar PV, Vasei M, Sadeghipour A. Visceral leishmaniasis: bone marrow biopsy findings. J Pediatr Hematol Onchol. 2007. 29(2): 77-80.
Asgari Q, Fakhar M, Motazedian MH, Cheraqali F, Banimostafavi E. Visceral leishmaniasis, an alarming rate of misdiagnosis. Iranian Red Crescent Med J. 2007; 95(1):45-7.
Sharma U, Redhu NS, Mathur P, Singh S. Re-emergence of visceral leishmaniasis in Gujarat, India.
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| Issue | Vol 6 No 4 (2011) | |
| Section | Articles | |
| Keywords | ||
| Visceral leishmaniasis Leishmania infantum Seroprevalence Direct agglutination test convenience PCR Iran | ||
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