Gene Diversity of Trichomonas vaginalis Isolates
,
Abstract
Background: Trichomonas vaginalis is protozoan parasite responsible for trichomoniasis and is more common in high-risk behavior group such as prostitute individuals. Interest in trichomoniasis is due to increase one's susceptibility to viruses such as herpes, human papillomavirus and HIV. The aim of this study was to find genotypic differences between the isolates.
Methods: Forty isolates from prisoners' women in Tehran province were used in this study. The random amplified polymorphic DNA (RAPD) technique was used to determine genetic differences among isolates and was correlated with patient's records. By each primer the banding pattern size of each isolates was scored (bp), genetic differences were studied, and the genealogical tree was constructed by using NTSYS software program and UPGMA method.
Results: The least number of bands were seen by using primer OPD8 and the most by using OPD3. Results showed no significant difference in isolates from different geographical areas in Iran. By using primer OPD1 specific amplified fragment with length 1300 base pair were found in only 8 isolates. All these isolates were belonged to addicted women; however, six belonged to asymptomatic patients and two to symptomatic ones.
Conclusion: There was not much genetic diversity in T vaginalis isolates from three different geographical areas.
Hobbs MM, Lapple DM, Lawing LF, Schwebke JR, Cohen MS, Swygard H, Atashili J, Leone PA, MillerWC, Seña AC. Methods for detection of Trichomonas vaginalis in the male partners of infected women: implications for control of trichomoniasis. J Clin Microbiol. 2006; 44(11):3994-9.
Swygard H, Seña AC, Hobbs M, Cohen M. Trichomoniasis: clinical manifestations, diagnosis, and management. Sex Transm Infect. 2004; 80: 91- 95.
Windell L, Rivera Vanissa A. Molecular characterization of T. vaginalis isolates from the Philippines. Parasitol Res. 2009; 106:105-110.
Hampl V, Vanácová S, Kulda J, Flegr J. Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains. BMC Evol Biol. 2001; 1:11.
Snipes LJ, Gamard PM, Narcisi EM, Beard CB, Lehmann T, and SecorWE. Molecular epidemiology of metronidazole resistance in a population of Trichomonas vaginalis clinical isolates. J Clin Microbiol. 2000; 38(8): 3004–3009.
Simoes-BarbosaA, Lobo TT,Xavier J, Carvalho SE, Leornadecz. Trichomo nas vaginalis: intrastrain polymorphisms within the ribosomal intergenic spacer do not correlate with clinical presentation. Exp Parasitol. 2005; 110(2):108-13.
Jamali R. Zareikar B. Kazemi A. Asgharzadeh M. Yousefee S. Estakhri R. Montazer S and Ghazanchaei A. Random amplified polymorphic DNA of Trichomonas vaginalis isolates from Tabriz, Iran. J Parasitic Dis. 2006; 30 (1): 53-57.
Garcia A, Exposto F, Prieto E, et al. Correia da Silva R. Association of Trichomonas vaginalis with sociodemographic factore and other STDs among female inmate in Lisbon. Int J STD AIDS. 2004; 15: 615-8.
Schwebke JR and Burgess D. Trichomoniasis. Clin Microbiol Rev. 2004; 17: 794-803.
Lawing LF, Hedges S R, and JR, Schwebke. Detection of trichomoniasis in vaginal and urine specimens from women by culture and PCR. J ClinMicrobiol. 2000; 38: 3585-3588.
Kaul P, Gupta I, Sehgal R, Malla N. Trichomonas vaginalis: random amplified polymorphic DNA analysis of isolates from symptomatic and asymptomatic women in India. Parasitol Int. 2004; 53(3):255-62.
Rojas L, Fraga J, Sariego I. Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation. Infect. Genetic and Evol. 2004; 4: 53-58.
Sheng Chong Z, Sheng Chong Y, RAPD analysis on different isolates of Trichomonas vaginalis. Bing ZaZhi. 2004; 30: 22(2): 90-3.
| Files | ||
| Issue | Vol 6 No 3 (2011) | |
| Section | Articles | |
| Keywords | ||
| Trichomonas vaginalis Prisoners RAPD Gene diversity | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
