Original Article

Toxoplasma gondii in Aborted Fetuses of Sheep in Ardebil Area, North-West of Iran


Background: One of the severe complications of toxoplasmosis is the induction of abortion in sheep, goats, and human beings. The rate of abortions related to toxoplasmosis in sheep varies between 10% and 23% in the United States and European countries. In Iran, Toxoplasma infection were diagnosed in aborted ovine fetuses between 13.5% and 69% in the brain samples based on PCR. The purpose of this study was to investigate the prevalence of abortions related to T. gondii among the aborted fetuses of sheep in Ardabil area, North-West of Iran.

Methods: The brains of 75 aborted sheep fetuses in Ardebil area were investigated with nested PCR method using fragments of the GRA6 gene during lambing seasons in 2014-2015. Meanwhile, thoracic and abdominal fluids of aborted fetuses and 200 serum samples collected from the herds affected by abortion were examined for the existence of anti-T. gondii IgG.

Results: For 48 out of the 75 fetal brain samples (64%), the results of nested PCR test were found to be positive. Furthermore, antibodies against Toxoplasma was observed in 136 (68%) collected samples of sheep and 21 samples (28%) of fetal fluids.

Conclusion: There is the significant role of T. gondii in the abortions of sheep in Ardabil area. Meanwhile, this condition can also be dangerous for human beings because of their consumption of sheep meat.

Dubey J P, Beattie C P . Toxoplasmosis of humans and animals. CRC press. florida. 2010; 1-80

Matin S, Shahbazi G, Tabrizian SH, Moradpour R, Feizi F, Piri - dogahe H. Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran. Korean J Parasitol.2017; 55(6): 607-611.

Matin S, Shahbazi G, Piri–doghahe H, Madani R, Kahnamoee F, Janngjou A, Matin E, Bageri A, Seid sadegi Z. Molecular study of toxoplasmosis in aborted placenta samples with Npcr method in Ardabil- Iran. IJBPAS. 2015; 4(11): 6386-6396.

Buxton D. Finlayson J. Experimental infection of pregnant sheep with Toxoplasma gondii: pathological and immunological observations on the placenta and foetus. J Comp Pathol. 1986; 96: 319-333.

Fayer R. Toxoplasmosis update and public health implication. Can vet J. 1981; 22: 344-352

Skjerve E, Waldeland H, Nesbakken T, Kapperud G. Risk factors for the presence of antibodies to Toxoplasma gondii in Norwegian slaughter lambs. Prev Vet Med. 1998; 35: 219–227.

Blewett D.A. The epidemiology of ovine toxoplasmosis. The interpretation of data for the prevalence of antibody in sheep and other host species. Br Vet J. 1983; 139: 537–545.

Danehchin L, Razmi G, Naghibi A. Isolation and genotyping of Toxoplasma gondii strains in ovine aborted fetuses in Khorasan Razavi Province, Iran. Korean J parasitol. 2016; 54(1):15.

Habibi GR, Imani AR, Gholami MR, Hablolvarid MH, Behroozikhah AM, Lotfi M, Kamalzade ME, Najjar EK, Esmaeil-Nia K, Bozorgi S.Detection and Identification of Toxoplasma gondii ,Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran. Iranian J Parasitol. 2012; 7 (3): 64-72.

Razmi GR, Ghezi K, Mahooti A, Naseri Z . A Serological Study and Subsequent Isolation of Toxoplasma gondii From Aborted Ovine Fetuses in Mashhad Area, Iranian. J Parasitol. 2010; 96(4): 812-814.

Khan A, Su C, German M, Storch GA, Clifford DB, Sibley LD. Genotyping of Toxoplasma gondii strains from immunocompromised patients reveals high prevalence of type I strains. J Clinical Microb. 2005; 43: 5881–5887.

Dubey jp.Toxoplasmosis in sheep- the last 20 year. Vet Parasitol. 2009; 163: 1-14.

Moazeni Jula F, Moazeni Jula G, Nowzari N, Kavari H, Hashemzadeh H. A serological and molecular study on Toxoplasma gondii infection in sheep and goat in Tabriz. Arch Razi Inst. 2013; 68(1):29-35

Seefeldt SL, Kirkbride CA, Dubey JP. Comparison of enzyme-linked immunosorbent assay, indirect fluorescent antibody test, and direct agglutination test for detecting Toxoplasma gondii antibodies in naturally aborted ovine fetuses. J Vet Diagn Invest. 1989; 1(2):124-7.

Dubey JP, Emond JP, Desmonts G, Anderson WR. Serodiagnosis of postnatally and prenatally induced toxoplasmosis in sheep. Am J Vet Res. 1987 48(8):1239-43.

Arthur MJ, Blewett DA. IFAT detection of IgG specific to Toxoplasma in thoracic fluids from aborted lambs: evaluation on routine diagnostic submissions. Vet Rec. 1988; 122(2):29-31.

Pereira JB, Quintanilla GA, P´erez-Pr´erez G, Alvarez G,Garcia,Collantes-Frenandez E, Ortega- Mora LM. Evolution of ovine abortion associated with Toxoplasma gondii in Spain by different diagnostic techniques. Vet Parasitol. 2004; 121: 33–43.

Movassaghi AR, Rassouli M, Fazaeli A, Salimi-Bejestani MR. Outbreak of ovine congenital toxoplasmosis in Iran, confirmed by different diagnostic methods. J Parasit Dis 2016; 40(1):152-6.

Sarkari B, Asgari Q, Mirzaee S. Evaluation of immunohistochemistry and PCR in diagnosis of Toxoplasma infection in tissues of human aborted fetuses. Zahedan J Res Med Sci. 2013; 15(12):42.

Sharif M,GHolami SH. Seroprevalence of Toxoplasma gondii in cattle, sheep and goat slaughtered for food in mazandaran province, Iran. J Anim Vet Adv. 2006; 5(3):188-190

Klun I, Djurković-Djaković O,Katić-Radivojević S , Nikolić A . Cross-sectional survey on Toxoplasma gondii infection in cattle, sheep and pigs in Serbia: seroprevalence and risk factors. Vet Parasitol. 2006;135: 121–131.

IssueVol 14 No 3 (2019) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijpa.v14i3.1482
Toxoplasma gondii Abortion Sheep Iran Nested PCR

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How to Cite
SHAHBAZI G, HOGHOOGHI RAD N, MADANI R, MATIN S, MORTAZAVI P, JANGJOU AH. Toxoplasma gondii in Aborted Fetuses of Sheep in Ardebil Area, North-West of Iran. Iran J Parasitol. 2019;14(3):430-435.