Diagnosis of Acute Toxoplasmosis by IgG and IgM Antibodies and IgG Avidity in Pregnant Women from Mashhad, Eastern Iran
Background: We aimed to evaluate the diagnosis of acute toxoplasmosis by IgG avidity test in pregnant women.
Methods: In this cross-sectional study, 250 blood samples were collected from pregnant women with the first month of their pregnancy referring to health centers of University in Mashhad during 2016. Samples were centrifuged at 3000 rpm for 5 min for separation of serum and were kept in the -20 until use. To detection of acute and chronic toxoplasmosis, anti-Toxoplasma antibodies (IgG and IgM, and IgG avidity tests were performed using ELISA. Then, data analyzed using SPSS software by Frequency, Pearson Chi-Square, Likelihood Ratio, and Exact tests. And P<0.05 was statistically considered as significant.
Results: Total prevalence of IgG and IgM was 23.2% and 7.2%, respectively. A significant correlation was observed between the mean age and IgG level (P<0.05). It was not found any correlation between the history of raw meat consumption, cats keeping, education, and residency site. Moreover, 16 people (6.4%) had IgM antibody, of which, 10 cases (62.5%) with low avidity for IgG and 1 people (6.2%) with moderate avidity and 5 cases (31.3%) with high avidity for IgG. Moreover, 76% of pregnant women were seronegative.
Conclusion: More than half of the women (62.5%) with positive IgM antibody in their serum had a low avidity for IgG which revealed an acute infection among pregnant women. Toxoplasma infection should be considered as an important factor that affects the pregnancy and IgG avidity as an important test for screening the women who need the treatment.
Grover CM, Thulliez P, Remington JS, Boothroyd JC. Rapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid. Journal of Clinical Microbiology. 1990;28(10):2297-301.
Jones JL, Kruszon-Moran D, Wilson M, McQuillan G, Navin T, McAuley JB. Toxoplasma gondii infection in the United States: seroprevalence and risk factors. American journal of epidemiology. 2001;154(4):357-65.
Cook A, Holliman R, Gilbert R, Buffolano W, Zufferey J, Petersen E, et al. Sources of toxoplasma infection in pregnant women: European multicentre case-control studyCommentary: Congenital toxoplasmosis—further thought for food. Bmj. 2000;321(7254):142-7.
Di Carlo P, Romano A, Schimmenti MG, Mazzola A, Titone L. Materno-fetal Toxoplasma gondii infection: critical review of available diagnostic methods. Infez Med. 2008;16(1):28-32.
Messier V, Levesque B, Proulx JF, Rochette L, Libman M, Ward B, et al. Seroprevalence of toxoplasma gondii among Nunavik Inuit (Canada). Zoonoses and public health. 2009;56(4):188-97.
Mostavafvi J JL. A systematic review of published studies on the epidemiology of Toxoplasmosis in Iran. Med J Isfahan Uni 2012;30(76).
Elmore SA, Jones JL, Conrad PA, Patton S, Lindsay DS, Dubey J. Toxoplasma gondii: epidemiology, feline clinical aspects, and prevention. Trends in parasitology. 2010;26(4):190-6.
Dunn D, Wallon M, Peyron F, Petersen E, Peckham C, Gilbert R. Mother-to-child transmission of toxoplasmosis: risk estimates for clinical counselling. The Lancet. 1999;353(9167):1829-33.
Avelino MM, Amaral WN, Rodrigues IM, Rassi AR, Gomes MB, Costa TL, et al. Congenital toxoplasmosis and prenatal care state programs. BMC infectious diseases. 2014;14(1):33.
Paquet C, Yudin MH, Allen VM, Bouchard C, Boucher M, Caddy S, et al. Toxoplasmosis in pregnancy: prevention, screening, and treatment. Journal of obstetrics and gynaecology Canada. 2013;35(1):78-9.
Montoya JG, Remington JS. Studies on the serodiagnosis of toxoplasmic lymphadenitis. Clinical infectious diseases. 1995;20(4):781-9.
Shariat Bahadory E, Sadraie J, Marsosi V, Mosavipour S. IgG Avidity ELISA Test for Diagnosis of congenital toxoplasmosis. Razi Journal of Medical Sciences. 2013;20(106):86-93.
Isenberg J. Novel diagnostic ocular toxoplasmosis biomarkers: McGill University Library; 2010.
Gharavi M, Jalali S, Khademvatan S, Heydari S. Detection of IgM and IgG anti-Toxoplasma antibodies in renal transplant recipients using ELFA, ELISA and ISAGA methods: comparison of pre-and post-transplantation status. Annals of Tropical Medicine & Parasitology. 2011;105(5):367-71.
Robert-Gangneux F, Dardé M-L. Epidemiology of and diagnostic strategies for toxoplasmosis. Clinical microbiology reviews. 2012;25(2):264-96.
Villard O, Breit L, Cimon B, Franck J, Fricker-Hidalgo H, Godineau N, et al. Comparison of four commercially available avidity tests for Toxoplasma gondii-specific IgG antibodies. Clinical and Vaccine Immunology. 2013;20(2):197-204.
Assmar M, Milaninia A, Amirkhani A, Yadegari D, Forghanparast K, Nahravanian H, et al. Seroepidemiological investigation of fascioliasis in northern Iran. Medical Journal of The Islamic Republic of Iran (MJIRI). 1991;5(1):23-7.
Keshavarz H, Nateghpour M, Eskandari S. A seroepidemiologic survey of Toxoplasmosis in Islamshahr district of Tehran, Iran. 2004.
Schenone H, Sandoval L, Contreras MdC, Salinas P, Rojas A. Epidemiology of toxoplasmosis in Chile. VII. Prevalence of human infection investigated by means of indirect hemagglutination reaction in the regions X, XI and XII. Boletin chileno de parasitologia. 1990;45(3-4):77-9.
Fallah M, Rabiee S, Matini M, Taherkhani H. Seroepidemiology of toxoplasmosis in primigravida women in Hamadan, Islamic Republic of Iran, 2004. 2008.
Badparva E. Prevalence of Toxoplasma gondii in pregnant women reffered to health-treatment centers of Khoramabad. 2001.
Daryani A, Sagha M. Seroepidemiology of toxoplasmosis in women referring to the laboratory of health center in Ardabil for premarital medical examinations. Journal of Ardabil University of Medical Sciences. 2004;4(3):19-25.
Ertug S, Okyay P, Turkmen M, Yuksel H. Seroprevalence and risk factors for toxoplasma infection among pregnant women in Aydin province, Turkey. BMC public health. 2005;5(1):66.
Alvarado-Esquivel C, Torres-Castorena A, Liesenfeld O, García-López C, Estrada-Martínez S, Sifuentes-Alvarez A, et al. Seroepidemiology of Toxoplasma gondii infection in pregnant women in rural Durango, Mexico. Journal of Parasitology. 2009;95(2):271-4.
Shah Moradi A, Daryani A, Hadji Zadeh E. A seroepidemiology of toxoplasmosis in Roudsar city. Daneshvar, Scientific-research Journal of Shahed University. 1997:15-6.
Berger F, Goulet V, Le Strat Y, Desenclos J-C. Toxoplasmosis among pregnant women in France: risk factors and change of prevalence between 1995 and 2003. Revue d'epidemiologie et de sante publique. 2009;57(4):241-8.
Nash J, Chissel S, Jones J, Warburton F, Verlander N. Risk factors for toxoplasmosis in pregnant women in Kent, United Kingdom. Epidemiology & Infection. 2005;133(3):475-83.
Rahbari AH, Keshavarz H, Shojaee S, Mohebali M, Rezaeian M. IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans. The Korean journal of parasitology. 2012;50(2):99.
|Issue||Vol 14 No 4 (2019)|
|IgG avidity Pregnant women Toxoplasmosis Iran|
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