Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER)
Background: The aim of present study was to evaluate antileishmanial effects of Lavandula angustifolia (L. angustifolia) and Rosmarinus officinalis (R. officinalis) medicinal plants essential oils and nano-emulsions on Leishmania major (L. major).
Methods: The present study was performed in Leishmaniasis Reference Lab at Mazandaran University of Medical Sciences, Iran during 2016-2017. The IC50 values were calculated in both the promastigote and amastigote stages in J774 macrophage in comparison with meglumine antimoniate (MA) as positive control. In addition, cytotoxicity effects of essential oils and nano-emulsions prepared from both plants against macrophages were evaluated.
Results: Both essential oil and nano-emulsion of Lavander and Rosemary showed anti-leishmania activity on promastigote with IC50=0.11 μl/mL, IC50=0.26 μl/mL, and IC50=0.08 μl/mL respectively. Moreover, during amastigote assay, Lavander and Rosemary essential oils and nano-emulsion were effective at least in concentration of 0.12 μl/mL and 0.06 µl/mL (P=0.0001) respectively, on mean infected macrophages (MIR) and amastigotes in macrophages (P=0.0001). Additionally, cytotoxicity assay against macrophage revealed no toxicity on the host cells at IC50 concentrations.Conclusion: The nano-emulsions of both plants were more effective than essential oil in both MIR and amastigote. However, in comparison with MA, the Lavander essential oil is more effective in reducing MIR. Rosemary nano-emulsion reduced MIR significantly more than MA in concentration of 0.25 μl/mL (P<0.001). Further investigations are recommended to evaluate the effect of these medicinal plants in murine models.
WHO. Report of a meeting of the WHO Expert Committee on the Control of Leishmaniases, Geneva. WHO Tech Series 2010; 949:23-129.
Fakhar M, Pazoki Ghohe H, Rasooli SA et al. Genetic diversity of Leishmania tropica strains isolated from clinical forms of cutaneous leishmaniasis in rural districts of Herat province, Western Afghanistan, based on ITS1-rDNA. Infect Genet Evol. 2016;41:120-127.
Mohebali M, Edrissian GH, Shirzadi MR et al. An observational study on the current distribution of visceral leishmaniasis in different geographical zones of Iran and implication to health policy. Travel Med Infect Dis. 2011;9(2):67-74.
Shirzadi MR, Esfahania SB, Mohebalia M et al. Epidemiological status of leishmaniasis in the Islamic Republic of Iran, 1983-2012. East Mediterr Health J. 2015;21(10):736-42.
Chakravarty J, Sundar S. Drug Resistance in Leishmaniasis. J Glob Infect Dis. 2010;2(2):167-76.
Sen R, Chatterjee M. Plant derived therapeutics for the treatment of leishmaniasis. Phytomedicine. 2011;18(12):1056-69.
Soosaraei M, Fakhar M, Hosseini Teshnizi S et al. Medicinal plants with promising antileishmanial activity in Iran: a systematic review and meta-analysis. Ann Med Surg (Lond). 2017;21:63-80.
Barati M, Sharifi I, Sharififar F. Antileishmanial activity of Artemisia aucheri, Ferula asafoetid and Gossypium hirsutum extracts on Leishmania major promastigotes in vitro. Ann Mil Health Sci Res. 2010; 8:166-172.
Mansour R, Haouas N, Ben Kahla-Nakbi A et al. The Effect of Vitis vinifera L. Leaves Extract on Leishmania infantum. Iran J Pharm Res. 2013;12(3):349-55.
Sakr SA, Lamfon HA. Protective effect of Rosemary (Rosmarinus officinalis) leaves extract on carbon tetrachloride -induced nephrotoxicity in Albino Rats. Life Sci J. 2012; 9(1):779-85.
al-Sereiti MR, Abu-Amer KM, Sen P. Pharmacology of Rosemary (Rosmarinus officinalis Linn) and its therapeutic potentials. Indian J Exp Biol. 1999;37(2):124-30.
Cavanagh HM, Wilkinson JM. Biological activities of lavender essential oil. Phytother Res. 2002;16(4):301-8.
Mirmajidi hashtjin A, Abbasi S. Nano-emulsification of orange peel essential oil using sonication and native gums. Food Hydrocol. 2015; 44: 40-48.
Davies NW. Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and carbowax 20M phases. J Chromato. 1990;503: 1-24.
Shokri A, Emami S, Fakhar M et al. In vitro antileishmanial activity of novel azoles (3-imidazolylflavanones) against promastigote and amastigote stages of Leishmania major. Acta Trop. 2017;167:73-78.
Sereno D, Lemesre JL. Use of an enzymatic micromethod to quantify amastigote stage of Leishmania amazonensis in vitro. Parasitol Res. 1997;83(4):401-3.
Shokri A, Akhtari J, Keighobadi M et al. Promising antileishmanial effectiveness of doxorubicin and Doxil against Leishmania major: an in vitro assay. Asian Pac J Trop Med. 2017;10(6):544-548.
Hadighi R, Mohebali M, Boucher P et al. Unresponsiveness to Glucantime Treatment in Iranian Cutaneous Leishmaniasis due to Drug- Resistant Leishmania tropica Parasites. PLoS Med. 2006;3(5):e162.
Hadighi R, Boucher P, Khamesipour A et al. Glucantime-resistant Leishmania tropica isolated from Iranian patients with cutaneous leishmaniasis are sensitive to alternative antileishmania drugs. Parasitol Res. 2007;101(5):1319-22.
Bahmani M, Saki K, Ezatpour B et al. Leishmaniosis phytotherapy: Review of plants used in Iranian traditional medicine on leishmaniasis. Asian Pac J Trop Biomed. 2015; 5: 695–701.
Oryan A. Plant-derived compounds in treatment of leishmaniasis. Iran J Vet Res. 2015;16(1):1-19.
Silva NCC, Fernandes Júnior A. Biological properties of medicinal plants: a review of their antimicrobial activity. Venom Anim Toxins Incl Trop Dis. 2010; 16: 402-413.
Zahiri M, Mohebali M, Khanavi M et al. Therapeutic effect of Scrophularia striata ethanolic extract against localized cutaneous leishmaniasis caused by Leishmania major (MRHO/IR/75/ER). Iran J Public Health. 2016;45(10):1340-1347.
Kontogianni VG, Tomic G, Nikolic I et al. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity. Food Chem. 2013;136(1):120-9.
Bozin B1, Mimica-Dukic N, Samojlik I et al. Antimicrobial and antioxidant properties of Rosemary and Sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. J Agric Food Chem. 2007;55(19):7879-85.
Seo YM, Jeong SH. Effects of Blending Oil of Lavander and Thyme on Oxidative Stress, Immunity, and Skin Condition in Atopic Dermatitis Induced Mice. J Korean Acad Nurs. 2015;45(3):367-77.
Babu US, Wiesenfeld PL, Jenkins MY. Effect of dietary Rosemary extract on cell-mediated immunity of young rats. Plant Foods Hum Nutr. 1999;53(2):169-74.
Hezarjaribi HZ, Elmi T, Dayer MS et al. A systematic review of the effects of Iranian pharmaceutical plant extracts on Giardia lamblia. Asian Pac J Trop Dis. 2015; 5(12):925-9.
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