Articles

Morphological and Morphometrical Description of Trichostrongylus Species Isolated from Domestic Ruminants in Khuzestan Province, Southwest Iran

Abstract

Backgrounds: Genus Trichostrongylus (Nematoda: Trichostrongylidae) is one of the most impor­tant zoonotic nematodes with wide geographic distribution in the world. The purpose of the present study was to describe morphological and morphometrical characteristics of male Trichostrongylus species, currently prevalent in domestic ruminants of Khuzestan Province, south­west Iran.

Methods: Gastro-intestinal organs of 1600 sheep, goats, cattle, and buffalos, slaughtered in Khuzestan Province, southwest Iran, were examined for infectivity with Trichostrongylus spe­cies. For examination and measurements of helminthes, Azo-carmine staining was per­formed, followed by camera lucida drawings of morphological characters and measurements of morphometrical criteria with a calibrated microscope. Using valid nematodes systematic keys, almost all the parasites were identified at the level of species.

Results: Overall, 114 animals were found infected with at least one species of Trichostrongy­lus. Considering morphological characteristics of male Trichostrongylus, six species were identi­fied including T. colubriformis, T. vitrinus, T. probolorus, T. capricola, T. longispicu­laris and   Trichostrongylus sp.

Conclusion: Although, compared to the previous decades, currently Trichostrongylus is much less prevalent in the domestic ruminants of the study area, but still different species occur in these animals. 

Eslami A, Meydani M, Maleki Sh Zargrzadeh A. Gastro-intestinal nematodes of wild sheep (Ovis orientalis) from Iran. J Wildl Dis. 1979; 15, 263-265.

Eslami A, Changizy E,MoghadamM. Prevalence of helminth infections in the cape hare (Lepus capensis) in Iran. Vet Res Comm. 2000; 24, 455-458.

Eslami A, Rahbari S, Nikbin S. Gastrointestinal nematodes of gazelle, Ga zella subguttrosa in Iran. Vet Parasitol. 1980; 7, 75-78.

Eslami A. Veterinary Helminthology (Nematoda, Acanthocephala). Vol. III, Tehran University Publication; 1997. 892 pp.

Ghadirian E, Arfaa F. Present status of Trichostrongylus in Iran. Am J Trop Med Hyg.1975; 24(6): 935-941.

Ghadirian E. Human infection with Trichostrongylus lerouxi (Biocca, Chabaud, And Ghadirian, 1974) in Iran. Am J Trop Med Hyg.1977; 26(6), 1212-1213.

Sahba GH, Arfaa F, Bijan H. Intestinal helminthiasis in the rural area of Khuzestan, southwest Iran. Ann Trop Med Parasitol. 1967; 61: 351-357.

Mowlavi GR, Mir Ahmadi H, Rezaeian M, Beigom Kia E, Ebrahimi Daryani N, Rokni MB, Golestan B, Shafiei R, Fereshtehnejad SM, Keramati MR. Prevalence of intestinal parasites in tribal parts of Khuzestan Province during 2005-07. Govaresh. 2008; 12(4):219-228.

Skrjabin KI, Shikhobalova NP, Shul'ts RS. Essentials of Nematodology. Vol. III. Trichostrongylids of Animals and Man. Israel Program for Scientific Translation; 1960. 704 pp.

Anderson RC. Nematode parasites of vertebrates: Their development and transmission. CABI Publishing, 2nd ed. Wallingford, UK; 2000. 650 pp.

Ghadirian E, Arfaa F, and Sadighian A, Human infection with Trichostrongylus capricola in Iran. Am J Trop Med Hyg.1974. 23: 1002- 1003.

Ghadirian E, Mofidi CH, Bijan H, Identification of different species of Trichostrongylus in Iran. Ann de Parasitol. 1968. 43: 467-476.

Yong TS. Lee JH, Sim S, Lee J, Min DY, Chai JY, Eom KS, Sohn WM, Lee SH, and Rim HJ. Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence. Korean J Parasitol. 2007; 45(1): 69-74.

Campbell BE, Nisbet AJ, Mulvenna J, Loukas A, Gasser RB. Molecular and phylogenetic characterization of cytochromes c from Haemonchus contortus and Trichostrongylus vitrinus (Nematoda: Trichostrongylida). Gene. 2008; 424(1-2): 121-9.

Cantacessi C, Mitreva M, Campbell BE, Hall RS, Young ND, Jex AR, Ranganthan S, Gasser RB. First transcriptomic analysis of the economically important parasitic nematode, Trichostrongylus colubriformis, using a next-generation sequencing approach. Infect Genet Evol. 2010; 10(8): 1199-207.

Baghaei M, Pestechian N, Salehi M, Kazemi B, Mirmohammad Sadeghi H. Identification of Trichostrongylus nematodes by PCR-RFLP in Isfahan. Journal of Isfahan Medical School. 2005; 23(77): 42-48. (In Persian)

Pestechian N, Baghaei M, Yosefi H. Study on Trichostrongylus eggs isolated from sheep by PCR-RFLP in Isfahan. Feyz. 2005; 35: 23-29. (In Persian).

Files
IssueVol 6 No 3 (2011) QRcode
SectionArticles
Keywords
Trichostrongylus Morphology Morphometry Ruminant Iran

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Ghasemikhah R, Mirhendi H, Kia E, Mowlavi G, Sarmadian H, Meshgi B, Golestan B, Mobedi I. Morphological and Morphometrical Description of Trichostrongylus Species Isolated from Domestic Ruminants in Khuzestan Province, Southwest Iran. Iran J Parasitol. 1;6(3):82-88.