Original Article

Evaluation of the Protoscolicidal Effects of Albendazole and Albendazole Loaded Solid Lipid Nanoparticles


Background: Protoscolex plays an important role in the development of hydatid cyst. Albendazole is one of the most effectual protoscolicidal agents for averting the reappearance of this disease, nonetheless, its low solubility and its low intestinal absorption necessitates the presence of a drug carrier to enhance its efficacy. In this study, the effect of albendazole and its nano-form on protoscolices in cultured media and in vivo was evaluated.

Methods: Microemulsion method was used to prepare the Solid lipid nanoparticles (SLNs) containing albendazole. Infected livers were collected from the Slaughterhouse of Ahvaz, Khuzestan in 2017. The protoscolices were stored in RPMI 1640 for one week, and their survival under the influence of albendazole and nano-albendazole on days 3 and 7 at concentrations of 250 and 500µg / ml was investigated. The live protoscolices exposed on day 3 at a concentration of 250 μg/ml were injected to mice for evaluation of pathogenicity. Three months later, after autopsy of the mice, the pathogenicity was evaluated.

Results: Protoscolicidal efficacy was highest in both concentrations on day 7 for albendazole and on day 5 for nano-albendazole. Following the autopsy of the mice, cyst growth was reported in all mice, and only two mice from the albandazole loaded SLNs group did not have any cyst.

Conclusion: Albendazole loaded SLNs showed a higher protoscolicidal property than the free form of this drug; therefore, the use of nano-formulation of this drug is recommended to prevent the onset of this disease.

Moazeni M, Alipour-Chaharmahali M-R. Echinococcus granulosus: in vitro effectiveness of warm water on protoscolices. Experimental parasitology. 2011;127(1):14-7.

Ammann RW, Eckert J. Cestodes: echinococcus. Gastroenterology Clinics of North America. 1996;25(3):655-89.

Filice C, Brunetti E. Use of PAIR in human cystic echinococcosis. Acta tropica. 1997;64(1):95-107.

Rogan MT, Hai WY, Richardson R, Zeyhle E, Craig PS. Hydatid cysts: does every picture tell a story? Trends in parasitology. 2006;22(9):431-8.

Horton R. Albendazole in treatment of human cystic echinococcosis: 12 years of experience. Acta tropica. 1997;64(1):79-93.

Pawłowski Z, Eckert J, Vuitton D, Ammann R, Kern P, Craig P, et al. Echinococcosis in humans: clinical aspects, diagnosis and treatment. WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. 2001:20-66.

Bekhti A, Schaaps J, Capron M, Dessaint J, Santoro F, Capron A. Treatment of hepatic hydatid disease with mebedazole: preliminary results in four cases. Br Med J. 1977;2(6094):1047-51.

Saimot A, Cremieux A, Hay J, Meulemans A, Giovanangeli M, Delaitre B, et al. Albendazole as a potential treatment for human hydatidosis. The Lancet. 1983;322(8351):652-6.

Morris D, Dykes P, Dickson B, Marriner S, Bogan J, Burrows F. Albendazole in hydatid disease. British medical journal (Clinical research ed). 1983;286(6359):103.

Morris D, Perez-Serrano J. Chemotherapy of hydatid disease. Journal of Antimicrobial Chemotherapy. 1992:362.

Mehnert W, Mäder K. Solid lipid nanoparticles: production, characterization and applications. Advanced drug delivery reviews. 2001;47(2):165-96.

Wen H, New R, Muhmut M, Wang J, Wang Y, Zhang J, et al. Pharmacology and efficacy of liposome-entrapped albendazole in experimental secondary alveolar echinococcosis and effect of co-administratration with cimetidine. Parasitology. 1996;113(02):111-21.

Kata M, Schauer M. Increasing the solubility characteristics of albendazole with dimethyl-beta-cyclodextrin. Acta pharmaceutica Hungarica. 1991;61(1):23.

Pensel PE, Castro S, Allemandi D, Bruni SS, Palma SD, Elissondo MC. Enhanced chemoprophylactic and clinical efficacy of albendazole formulated as solid dispersions in experimental cystic echinococcosis. Veterinary parasitology. 2014;203(1):80-6.

Kaur I, Verma M. Exploring solid lipid nanoparticles for intranasal administration of streptomycin. Int J Pharma. 2011;1(2):110-7.

Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clinical microbiology reviews. 2004;17(1):107-35.

Smego RA, Sebanego P. Treatment options for hepatic cystic echinococcosis. International journal of infectious diseases. 2005;9(2):69-76.

Sinan T, Sheikh M, Chisti FA, Al Saeed O, Sheikh Z, Hira P, et al. Diagnosis of abdominal hydatid cyst disease: the role of ultrasound and ultrasound-guided fine needle aspiration cytology. Medical Principles and Practice. 2002;11(4):190-5.

Gharbi HA, Hassine W, Brauner M, Dupuch K. Ultrasound examination of the hydatic liver. Radiology. 1981;139(2):459-63.

Macpherson C, Bartholomot B, Frider B. Application of ultrasound in diagnosis, treatment, epidemiology, public health and control of Echinococcus granulosus and E. multilocularis. Parasitology. 2003;127(S1):S21-S35.

Shambesh M, Macpherson C, Beesley W, Gusbi A, Elsonosi T. Prevalence of human hydatid disease in northwestern Libya: a cross-sectional ultrasound study. Annals of tropical medicine and parasitology. 1992;86(4):381-6.

Moro PL, Garcia HH, Gonzales AE, Bonilla JJ, Verastegui M, GilmanMD RH. Screening for cystic echinococcosis in an endemic region of Peru using portable ultrasonography and the enzyme-linked immunoelectrotransfer blot (EITB) assay. Parasitology research. 2005;96(4):242-6.

Wang Y, He T, Wen X, Li T, Waili T, Zhang W, et al. Human cystic echinococcosis in two Mongolian communities in Hobukesar (China) and Bulgan (Mongolia). Transactions of the Royal Society of Tropical Medicine and Hygiene. 2005;99(9):692-8.

Jelowdar A, Rafiei A, Abbaspour MR, Rashidi I, Rahdar M. Efficacy of combined albendazol and praziquntel and their loaded solid lipid nanoparticles components in chemoprophylaxis of experimental hydatidosis. Asian Pacific Journal of Tropical Biomedicine. 2017.

Lv H, Jiang Y, Liao M, Sun H, Zhang S, Peng X. In vitro and in vivo treatments of Echinococcus granulosus with Huaier aqueous extract and albendazole liposome. Parasitology research. 2013;112(1):193-8.

Sahin M, Eryilmaz R, Bulbuloglu E. The effect of scolicidal agents on liver and biliary tree (experimental study). Journal of Investigative Surgery. 2004;17(6):323-6.

Topcu O, Sumer Z, Tuncer E, Aydin C, Koyuncu A. Efficacy of chlorhexidine gluconate during surgery for hydatid cyst. World journal of surgery. 2009;33(6):1274-80.

Brahim MB, Nouri A, Ksia A, El Ezzi O, Krichene I, Mekki M, et al. Management of multiple echinococcosis in childhood with albendazole and surgery. Journal of pediatric surgery. 2008;43(11):2024-30.

Xue-Nong Z, Jing-Hong G, Li-Hua T, Qiang Z. Studies on the drug-loading mechanism of polybutylcyanocrylate nanoparticle and its stability of thermodynamics. Current Nanoscience. 2008;4(1):59-61.

Pérez-Serrano J, Casado N, Rodriguez-Caabeiro F. The effects of albendazole and albendazole sulphoxide combination-therapy on Echinococcus granulosus in vitro. International journal for parasitology. 1994;24(2):219-24.

Mohammadnejad F, Ghaffarifar F, Dalimi A, Hassan ZM. In Vitro Effects of Artemether, Artemisinine, Albendazole, and Their Combinations on Echinococcus granolosus Protoscoleces. Jundishapur Journal of Natural Pharmaceutical Products. 2016;11(1).

Rahimi MT, Ahmadpour E, Esboei BR, Spotin A, Koshki MHK, Alizadeh A, et al. Scolicidal activity of biosynthesized silver nanoparticles against Echinococcus granulosus protoscolices. International Journal of Surgery. 2015;19:128-33.

Polat E, Aslan M, Cakan H, Saribas S, Ipek T, Kocazeybek B. The effects of albendazole and povidone iodine for hydatid cysts protoscoleces, ın-vitro and-vivo. African Journal of Microbiology Research. 2009;3(11):743-6.

Rafiei A, Pipelzadeh MH, Jahanshahi A, Salim MRE. Comparing the effectiveness of albendazole and combination of albendazole and praziquantel in experimental hydatidosis. Archives of Clinical Infectious Diseases. 2009;4(1):9-12.

Soltani S, Rafiei A, Ramezani Z, Abbaspour MR, Jelowdar A, Kahvaz MS. Evaluation of the hydatid cyst membrane permeability of albendazole and albendazole sulfoxide-loaded solid lipid nanoparticles. Jundishapur Journal of Natural Pharmaceutical Products. 2016(Inpress).

IssueVol 14 No 1 (2019) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijpa.v14i1.726
Albendazole Nanoparticles Echinococcus granulosus Hydatid cyst

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How to Cite
AMINPOUR S, RAFIEI A, JELOWDAR A, KOUCHAK M. Evaluation of the Protoscolicidal Effects of Albendazole and Albendazole Loaded Solid Lipid Nanoparticles. Iran J Parasitol. 2019;14(1):127-135.