Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran
-
Fariba BERENJI
,
Seyed Aliakbar SHAMSIAN
,
Marziyeh NOURI DALOEE
,
Seyed Hossein FATTAHI MASOOM
,
Elham MOGHADDAS
Abstract
Background: Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically.
Methods: Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015-2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit I (nad1).
Results: Overall, 55 out of 60 Echinococcus granulosus cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified.
Conclusion: Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region.
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| Files | ||
| Issue | Vol 14 No 1 (2019) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijpa.v14i1.717 | |
| Keywords | ||
| Hydatid Human Strain Iran Echinococcus granulosus | ||
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