Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran
-
Esmaeil FALLAH
,
Zahra JAFARPOUR
,
Mahmoud MAHAMI-OSKOUEI
,
Ali HAGHIGHI
,
Maryam NIYYATI
,
Adel SPOTIN
,
Aram KHEZRI
Abstract
Background: Acanthamoeba is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize Acanthamoeba isolates from surface resting waters, in Northwest Iran.
Methods: Samples were collected from twenty-two different areas, between May and Sep 2014. After filtration, samples were cultivated on non-nutrient agar. The extracted DNAs were amplified and sequenced using partial 18S rRNA in order to genotype and phylogenetic analyses.
Results: Thirty-four (68%) out of 50 collected samples were positive for free-living amoebae based on both culture and morphological characterizations but 28 samples were identified as Acanthamoeba spp. by PCR. Sequentially, one isolate was identified as A. lenticulata, (T5) (AN: KP940443, identity 99.7%-100%, and divergence 0.3%) whilst other sequenced isolates identified Acanthamoeba spp. (AN: KP940444-45) as very similar to A. rhysodes and A. royreba with identity 100% and divergence 0%.
Conclusion: Surface resting waters in Northwest Iran, were potentially contaminated with pathogenic amphizoic protozoan. Further studies will be required to determine other Acanthamoeba species and genotypes in the region.
Schuster FL, Visvesvara GS. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol. 2004; 34(9):1001-27.
Khan NA. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev. 2006; 30(4): 564-95.
Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev. 2003; 16(2): 273-307.
Greub G, Raoult D. Microorganisms resistant to free-living amoebae. Clin Microbiol Rev. 2004; 17(2): 413-33.
Pussard M, Pons R. Morphologie de la paroi kystiqueet taxonomie du genre Acanthamoeba (Protozoa, Amoebida). Protistologica. 1977; 13(4): 557–98.
Schmidt GD, Roberts LS. Foundations of parasitology, 8th edition. McGraw Hill, New York. 2009; 118-19.
King CH, Shotts EB Jr, Wooley RE, et al. Survival of coliforms and bacterial pathogens within protozoa during chlorination. Appl Environ Microbiol. 1988; 54(12): 3023-33.
Michel R, Hauröder B. Isolation of an Acanthamoeba strain with intracellular Burkholderia pickettii infection. Zentralbl Bakteriol. 1997; 285(4): 541-57.
Rowbotham TJ. Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J Clin Pathol. 1980; 33(12): 1179-83.
Booton GC, Rogerson A, Bonilla TD, et al. Molecular and physiological evaluation of subtropical environmental isolates of Acanthamoeba spp., causal agent of Acanthamoeba keratitis. J Eukaryot Microbiol. 2004; 51(2): 192-200.
Parija SC, Prakash MR, Rao VA, et al. Acanthamoeba keratitis in Pondicherry. J Commun Dis. 2001; 33(2): 126-9.
Stehr-Green JK, Bailey TM, Visvesvara GS. The epidemiology of Acanthamoeba keratitis in the United States. Am J Ophthalmol. 1989; 107(4): 331-6.
Murakawa GJ, McCalmont T, Altman J et al. Disseminated acanthamebiasis in patients with AIDS: a report of five cases and a review of the literature. Arch Dermatol. 1995; 131(11): 1291-6.
Niyyati M, Lorenzo-Morales J, Rezaie S et al. Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran. Exp Parasitol. 2009; 121(3): 242-5.
Rezaeian M, Niyyati M, Farnia S et al. Isolation of Acanthamoeba spp. from different environmental sources. Iran J Parasitol. 2008; 3(1): 44-7.
Maghsood AH, Sissons J, Rezaian M et al. Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. J Med Microbiol. 2005; 54(Pt8): 755-9.
Hooshyar H, Hosseinbigi B, Saraie M et al. Genotyping of Acanthamoeba isolated from surface and stagnant waters of Qazvin, central Iran. Iran Red Crescent Med J. 2013; 15(6): 536-8.
Lasjerdi Z, Niyyati M, Haghighi A et al. Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran. Parasitol Res. 2011; 109(3): 575-80.
Nazar M, Haghighi A, Niyyati M et al. Genotyping of Acanthamoeba isolated from water in recreational areas of Tehran, Iran. J Water Health. 2011; 9(3): 603-8.
Lekkla A, Sutthikornchai C, Bovornkitti S et al. Free-living ameba contamination in natural hot springs in Thailand. Southeast Asian J Trop Med Public Health. 2005 ;36 Suppl 4:5-9.
Khan NA. Pathogenesis of Acanthamoeba infections. Microb Pathog. 2003; 34(6): 277-85.
Cao Z, Jefferson DM, Panjwani N. Role of carbohydrate-mediated adherence in cytopathogenic mechanisms of Acanthamoeba. J Biol Chem. 1998; 273(25): 15838-45.
Lorenzo-Morales J, López-Darias M, Martínez-Carretero E et al. Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco. Exp Parasitol. 2007; 117(1): 74-9.
Jeong HJ, Yu HS. The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea. Korean J Parasitol. 2005; 43(2): 47-50.
Niyyati M, Lorenzo-Morales J, Rezaie S et al. First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran. Exp Parasitol. 2010; 126(1): 89-90.
McAllum P, Bahar I, Kaiserman I et al. Temporal and seasonal trends in Acanthamoeba keratitis. Cornea. 2009; 28(1): 7-10.
Derda M, Wojtkowiak-Giera A, Hadaś E. Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates. Acta Parasitol. 2014; 59(3): 472–7.
Nuprasert W, Putaporntip C, Pariyakanok L et al. Identification of a novel T17 genotype of Acanthamoeba from environmental isolates and T10 genotype causing keratitis in Thailand. J Clin Microbiol. 2010; 48(12): 4636-40.
Rahdar M, Niyyati M, Salehi M et al. Isolation and genotyping of Acanthamoeba strains from environmental sources in Ahvaz City, Khuzestan Province, Southern Iran. Iran J Parasitol. 2012; 7(4): 22-6.
Badirzadeh A, Niyyati M, Babaei Z et al. Isolation of free-living amoebae from sarein hot springs in ardebil province, iran. Iran J Parasitol. 2011; 6(2): 1-8.
Barete S, Combes A, de Jonckheere JF, et al. Fatal disseminated Acanthamoeba lenticulata acanthamebiasis in a heart transplant patient. Emerg Infect Dis. 2007; 13(5): 736-8.
van Zyl LM, Andrew N, Chehade M et al. Acanthamoeba lenticulata keratitis in a hard contact lens wearer. Clin Exp Ophthalmol. 2013; 41(8): 810-2.
Gardner HA, Martinez AJ, Visvesvara GS et al. Granulomatous amebic encephalitis in an AIDS patient. Neurology. 1991; 41(12): 1993-5.
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| Issue | Vol 12 No 3 (2017) | |
| Section | Original Article(s) | |
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| Acanthamoeba Surface water PCR Iran | ||
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