Molecular Studies on Cystic Echinococcosis of Camel (Camelus dromedarius) and Report of Echinococcus ortleppi in Iran
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Abstract
Background: Cystic echinococcosis (CE) is one of the most important zoonotic diseases; caused by different genotypes of Echinococcus spp. Camels have an important role in transmission cycle of E. granulosus especially, in desert areas. This study aimed to investigate molecular characterization of hydatid cysts isolates from one-humped camel (Camelus dromedarius) and to show its molecular and phylogenic status in this important CE host in the central part of Iran.
Methods: Twenty hydatid cyst samples (14 fertile and 6 calcified) were collected from 56 slaughtered camels in Central part of Iran. Extraction of DNA from 14 fertile samples was achieved followed by molecular studies on two mitochondrial genes (nad1 and cox1).
Results: Blast and phylogenetic analysis on sequenced genes showed the presence of G1 (28.6%), G3 (28.6%) and G6 (35.7%) genotypes in the samples. However, one sample was detected as E. ortleppi (G5) with 99% homology with G5 isolated from camel in Egypt (AB921055) and Sudan (JX912709).
Conclusion: Presence of E. ortleppi, originally the cattle genotype, is reported for the first time in Iran. Due to the potential of infecting human by E. ortleppi; more attention should be paid to this zoonotic genotype in this region.
Budke CM, Deplazes P, Torgerson PR. Global socioeconomic impact of cystic echinococcosis. Emerg Infect Dis. 2006; 12(2):296-303.
Fasihi Harandi M, Budke CM, Rostami S. The monetary burden of cystic echinococcosis in Iran. PLoS Negl Trop Dis. 2012; 6(11):e1915.
Sadjjadi SM. Present situation of echinococcosis in the middle east and arabic north africa. Parasitol Int. 2006; 55 Suppl:S197-202.
Rokni MB. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009;4:1-16.
Nakao M, Lavikainen A, Yanagida T et al. Phylogenetic systematics of the genus Echinococcus (cestoda: Taeniidae). Int J Parasitol. 2013; 43(12-13):1017-29.
Nakao M, McManus DP, Schantz PM et al. A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology. 2007; 134(Pt 5):713-22.
Romig T, Ebi D, Wassermann M. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Vet Parasitol. 2015; 213(3-4):76-84.
Yanagida T, Mohammadzadeh T, Kamhawi S et al. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the middle east. Parasitol Int. 2012; 61(4):599-603.
Ahmadi NA. Hydatidosis in camels (Camelus dromedarius) and their potential role in the epidemiology of Echinococcus granulosus in Iran. J Helminthol. 2005; 79(2):119-25.
Ahmadi N, Dalimi A.. Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infect Genet Evol. 2006; 6(2):85-90.
Farhadi M, Fazaeli A, Haniloo A. Genetic characterization of livestock and human hydatid cyst isolates from northwest Iran, using the mitochondrial cox1 gene sequence. Parasitol Res. 2015; 114(12):4363-70.
Fadakar B, Tabatabaei N1, Borji H et al. Genotyping of Echinococcus granulosus from goats and sheep indicating G7 genotype in goats in the northeast of Iran. Vet Parasitol. 2015; 214(1-2):204-7.
Parsa F, Fasihi Harandi M, Rostami S et al. Genotyping Echinococcus granulosus from dogs from western Iran. Exp Parasitol. 2012; 132(2):308-12.
Pezeshki A, Akhlaghi L, Sharbatkhori M et al. Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil province, northwest Iran. J Helminthol. 87(4):387-91.
Rajabloo M, Hosseini SH, Jalousian F. Morphological and molecular cha-racterisation of Echinococcus granulosus from goat isolates in Iran. Acta Trop. 2012; 123(2):67-71.
Ranjbar-Bahadori S, Lotfollahzadeh S, Vaezi G et al. Epidemiological study of the human cystic echinococcosis in Iran. Res J Parasitol. 2008;3:130-136.
Rostami S, Shariat Torbaghan S, Dabiri S et al. Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tissue samples of human isolates in Iran. Am J Trop Med Hyg. 2015; 92(3):588-94.
Sharafi SM, Rostami-Nejad M, Moazeni M et al. Echinococcus granulosus genotypes in Iran. Gastroenterol Hepatol Bed Bench. 2014; 7(2):82-8.
Thompson RA, Lymbery AJ. Echinococcus and hydatid disease. Cab International; 1995.
Harandi MF, Hobbs RP, Adams PJ et al. Molecular and morphological cha-racterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology. 2002; 125(Pt 4):367-73.
Hajialilo E, Harandi MF, Sharbatkhori M, et al. Genetic characterization of Echinococcus granulosus in camels, cattle and sheep from the south-east of Iran indicates the presence of the g3 genotype. J Helminthol. 2012; 86(3):263-70.
Moghaddas E, Borji H, Naghibi A et al. Molecular genotyping of Echinococcus granulosus from dromedaries (Camelus dromedarius) in eastern Iran. J Helminthol. 2015; 89(1):100-4.
Pestechian N, Hosseini Safa A, Tajedini M et al. Genetic diversity of Echinococcus granulosus in center of Iran. Korean J Parasitol. 2014; 52(4):413-8.
Sharbatkhori M, Fasihi Harandi M, Mirhendi H et al. Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran. Parasitol Res. 2011; 108(3):521-7.
Sharbatkhori M, Mirhendi H, Harandi MF et al. Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camels. Exp Parasitol. 2010; 124(4):373-9.
Kia EB, Rahimi H, Sharbatkhori M, Talebi A et al. Genotype identification of human cystic echinococcosis in Isfahan, central Iran. Parasitol Res. 2010; 107(3):757-60.
Sadjjadi SM, Mikaeili F, Karamian M et al. Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013; 43(11):875-7.
Shahnazi M, Hejazi H, Salehi M et al. Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop. 2011; 117(1):47-50.
Mehrabani D, Oryan A, Sadjjadi SM. Prevalence of Echinococcus granulosus infection in stray dogs and herbivores in Shiraz, Iran. Vet Parasitol. 1999; 86(3):217-20.
Seimenis A. Overview of the epidemiological situation on ech-inococcosis in the Mediterranean region. Acta Trop. 2003; 85(2):191-5.
Fallah M, Taherkhani H, Sadjjadi M. Echinococcosis in the stray dogs in Hamadan, west of Iran. Iran J Med Sci. 1995;20:170-172.
Mbaya H, Magambo J, Njenga S et al. Echinococcus spp. In central Kenya: A different story. Parasitol Res. 2014; 113(10):3789-94.
Ahmed ME, Eltom KH, Musa NO et al. First report on circulation of Echinococcus ortleppi in the one humped camel (Camelus dromedaries), Sudan. BMC Vet Res. 2013; 9:127.
Amer S, Helal IB, Kamau E et al. Molecular characterization of Echinococcus granulosus sensu lato from farm animals in Egypt. PLoS One. 2015; 10(3):e0118509.
Grenouillet F, Umhang G, Arbez-Gindre F et al. Echinococcus ortleppi infections in humans and cattle, France. Emerg Infect Dis. 2014; 20(12):2100-2.
Alvarez Rojas CA, Romig T, Lightowlers MW. Echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge. Int J Parasitol. 2014; 44(1):9-18.
Sharma M, Sehgal R, Fomda BA et al. Molecular characterization of Echinococcus granulosus cysts in north indian patients: Identification of g1, g3, g5 and g6 genotypes. PLoS Negl Trop Dis. 2013; 7(6):e2262.
Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54(2):165-73.
Bowles J, McManus DP. Nadh dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. Int J Parasitol. 1993; 23(7):969-72.
Kearse M, Moir R, Wilson A et al. Geneious basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012; 28(12):1647-9.
Hall TA. Bioedit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/nt. Nucleic Acids Symposium Series. 1999:95.
Kumar S, Stecher G, Tamura K. Mega7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016; 33(7):1870-4.
Thompson RC. The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol. 2008; 119(4):439-46.
Sharifiyazdi H, Oryan A, Ahmadnia S et al. Genotypic characterization of Iranian camel (Camelus dromedarius) isolates of Echinoccocus granulosus. J Parasitol. 2011; 97(2):251-5.
Zhang L, Eslami A, Hosseini SH, et al. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. Am J Trop Med Hyg. 1998; 59(1):171-4.
Elham M, Hassan B, Ghasem NA et al. Epidemiological study of hydatidosis in the dromedaries (Camelus dromedarius) of different regions of Iran. Asian Pac J Trop Biomed. 2014; 4(Suppl 1):S148-51.
Mirzaei M, Rezaei H, Nematollahi A et al. Survey of hydatidosis infection in slaughtered camel (Camelus dromedarius) in Tabriz area, northwest Iran. J Parasit Dis. 2016; 40(2):444-7.
Aaty HE, Abdel-Hameed DM, Alam-Eldin YH et al. Molecular genotyping of Echinococcus granulosus in animal and human isolates from Egypt. Acta Trop. 2012; 121(2):125-8.
Bardonnet K, Piarroux R, Dia L et al. Combined eco-epidemiological and molecular biology approaches to assess Echinococcus granulosus transmission to humans in mauritania: Occurrence of the 'camel' strain and human cystic echinococcosis. Trans R Soc Trop Med Hyg. 2002; 96(4):383-6.
Bardonnet K, Benchikh-Elfegoun MC, Bart JM et al. Cystic echinococcosis in Algeria: Cattle act as reservoirs of a sheep strain and may contribute to human contamination. Vet Parasitol. 2003; 116(1):35-44.
Dinkel A, Njoroge EM, Zimmermann A et al. A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in Eastern Africa. Int J Parasitol. 2004; 34(5):645-53.
Tashani OA, Zhang LH, Boufana B al. Epidemiology and strain characteristics of Echinococcus granulosus in the Benghazi area of eastern Libya. Ann Trop Med Parasitol. 2002; 96(4):369-81.
Balbinotti H, Santos GB, Badaraco J et al. Echinococcus ortleppi (G5) and Echinococcus granulosus sensu stricto (G1) loads in cattle from southern Brazil. Vet Parasitol. 2012; 188(3-4):255-60.
Andresiuk MV, Gordo FP, Saarma M et al. Echinococcus granulosus genotype g1 dominated in cattle and sheep during 2003-2006 in Buenos Aires Province, an endemic area for cystic echinococcosis in Argentina. Acta Trop. 2013; 127(2):136-42.
Casulli A, Manfredi MT, La Rosa G et al. Echinococcus ortleppi and e. Granulosus g1, g2 and g3 genotypes in Italian Bovines. Vet Parasitol. 2008; 155(1-2):168-72.
Boufana B, Stidworthy MF, Bell S et al. Craig PS. Echinococcus and Taenia spp. from captive mammals in the United Kingdom. Vet Parasitol. 2012; 190(1-2):95-103.
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| Issue | Vol 12 No 3 (2017) | |
| Section | Original Article(s) | |
| Keywords | ||
| Echinococcus ortleppi Mitochondrial DNA genes Phylogenetic analysis Camel Iran | ||
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