Haemolymph Components of Infected & None Infected Lymnaea snails with Xiphidiocercariae

  • AA Saboor Yaraghi Dept. of Nutrition and Biochemistry, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • A Farahnak Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • MR Eshraghian Dept. of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Tehran, Iran
Cercaria, Lymnaea, Haemolymph, Xiphidiocercariae


Background: In this study the haemolymph components of infected and none infected Lymnaea gedrosiana with xiphidiocercaria larvae was compared.Methods: Five hundred Fifty Lymnaea snails were collected from Ilam and Mazandaran prov­inces, Iran, during 2008-2009. The snails were transported to the lab at Tehran University of Medi­cal Sciences and their cercarial sheddings were studied. Haemolmyphs of snails were ex­tracted and cells were counted using haemocytometer and cell-surface carbohydrate were recog­nized by conjugated lectin (Lentil). Haemolymph protein concentrations were measured by Brad­ford protein assay method and soluble protein compositions were determined on sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE).Result: From the 550 examined Lymnaea snails for cercariae, 27 snails were infected with xiphidiocer­cariae. Mean of haemolymph cells (haemocyte) number were obtained 93480±2.43 (cells/ml) for none infected snails (25 snail) and 124560±2800 (cells/ml) for infected snails (25 snail). Mannose carbohydrate was recognized on haemocyte of none infected and infected snails.  Mean of protein concentration of haemolymph plasma was obtained as 1354 ± 160 μg/ml (1.4 mg/ml) for none infected snails (25 snails) and 1802±138 μg/ml (1.8 mg/ml) for infected snail (25 snails). Comparing to none infected snails, the SDS-PAGE results of haemolymph plasma of infected snails, showed an extra protein band (70 kDa). The results showed a significant differ­ence between the amounts and the kinds of proteins in haemolymph of infected and none infected snails.Conclusion: This information might be useful to understand of parasite detection, adhesion, engulf­ment and antigen agglutination by snail.


Chingwena G, Mukaratirwa S, Kristensen TK, Chimbari M. Larval trematode infections in freshwater snails from the Highveld and Lowveld areas of Zimbabwe . J Helminthology.2002; 76: 283–293.

Bezerra JCB, Becker W, Zelck UE. A comparative study of the organic acid content of the hemolymph of schistosoma mansoni-resistant and susceptible strains of biomphalaria glabrata. Mem Inst Oswaldo Cruz, Rio de Janeiro. 1997; 92(3): 421-425.

Rupprecht H, Becker W, Schwanbek A. Alterations in hemolymph components in Biomphalaria glabrata during longterm infection with Schistosoma mansoni. Parasitol Res. 1989; 75(3):233-7.

Loker ES. Alterations in Biomphalaria glabrata plasma induced by infection with the digenetic trematode Echinostoma paraensei. J Parasitol. 1987; 73(3):503-13.

Farahnak A, Dabagh N. Adhesion of Cercaria (Larva of Helminth Parasites) to Host by Lectins- carbohydrates bonds as a Model for Evaluation of Schistosoma Entrance Mechanisms in Cercarial Dermatitis. Iranian J Publ Health. 2008; 37(2): 59-63.

Stewart CS. The Trematodes. M.C. Dubuque, Iowa: Brown Company Publisher; 1970.

Malek ETA. Laboratory guide and notes for medical malacology. Minneapolis: Burgess Pub. Co.; 1962.

Caprette DR. Experimental bioscience; Laboratory methods: Using a Counting Chamber [online]; 2007. Available from: http://www.ruf.rice.edu/~bioslabs/meth ods/microscopy/cellcounting.html.

Maizels RM, Blaxter ML, Robertson BD, Selkirk ME. Parasite antigen and parasite genes: A laboratory manual for molecular parasitology.1st ed. Cambridge: Cambridge University Press; 1991,pp 93-94.

Moema EB, King PH, Baker C. Cercariae developing in Lymnaea natalensis Krauss, 1848 collected in the vicinity of Pretoria, Gauteng Province, South Africa. J Vet Res. 2008; 75(3):215-23.

Narayanan R, Venkateswararao P. Effect of xiphidiocercarial infection on oxidation of glycolytic and Krebs cycle intermediates in Lymnaea luteola (Mollusca). J Invertebr Pathol.1980; 36(1): 21-24.

Reddy BR, Rao PV. Effect of xiphidiocercarial infection on the purine nucleotide cycle activity in the freshwater pulmonate snail, Lymnaea luteola. J Invertebr Pathol.1992; 60(2): 117-120.

El-Emam MA, Ebeid FA. Effect of Schistosoma mansoni infection, starvation and molluscicides on acid phosphate, transaminases and total protein in tissues and hemolymph of Biomphalaria alexandrina. J Egypt Soc Parasitol. 1989; 19(1):139-47.

Sminia T, Knaap W. The internal defence system of the freshwater snail. Developmental & Comparative Immunology. 1981; 5, Supplement 1: 87-97.

Mohamed AH. Characterization of surface lectins binding and SDS-PAGE protein patterns of Biomphalaria alexandrina haemocytes infected with Schistosoma mansoni. J Egypt Soc Parasitol. 2005; 35(2):615-30.

Weis WI, Drickamer K. Structural basis of lectin-carbohydrate recognition. Annu Rev Biochem. 1996; 65:441-73.

Amen RI, Tijnagel JM, Knaap WPW, Meuleman EA, Klerk ES, Sminia T. Effects of Trichobilharzia ocellata on hemocytes of Lymnaea stagnalis. Dev Comp Immunol. 1991; 15(3):105-15.

Knaap WPW, Doderer A, Boerrigter- Barendsen H, Sminia T. Some Properties of an Agglutinin in the Haemolymph of the Pond snail Lymnaea stagnalis. Biol bull.1982; 162: 404-412.

Horák P, Knaap WPW. Lectins in snail-trematode immune interactions: a review. FoliaParasitológica.1997; 44:161-172.

How to Cite
Saboor Yaraghi A, Farahnak A, Eshraghian M. Haemolymph Components of Infected & None Infected Lymnaea snails with Xiphidiocercariae. Iran J Parasitol. 6(1):86-91.