Articles

Detection and Molecular Characterization of Cryptosporidium species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran using nested-PCR-RFLP

Abstract

Background: The aim of this study was to detect and characterize Cryptosporidium spp. in water sam­ples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran .

Meth­ods: Thirty water samples were collected  from November 2009 to May 2010. Each sample con­tained 10 liters of water. We used the SSU rRNA-based PCR-RFLP technique.

Results: Out of thirty samples examined, 6 (20%) were positive for different Cryptosporidium spp. Restriction pattern analysis showed that C. parvum has been the most prevalent genotype, fol­lowed by C. hominis  and C. canis , respectively. In this area, the higher prevalence of C. par­vum compared with other genotypes is consistent with the distribution of cattle.

Conclusion: Farm animals, particularly cattle arethe main source of cryptosporidial contamina­tion for recreational waters in this area.

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IssueVol 6 No 1 (2011) QRcode
SectionArticles
Keywords
Recreational waters Cryptosporidium Genotyping Nested-PCR-RFLP Iran
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How to Cite
1.
Manouchehri Naeini K, Asadi M, Hashemzade Chaleshtori M. Detection and Molecular Characterization of Cryptosporidium species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran using nested-PCR-RFLP. Iran J Parasitol. 1;6(1):20-27.