Short Communication

In Vitro Assessment of Balanites aegyptiaca Fruit Methanolic Extract on the Adult Toxocara canis


Background: The effectiveness of anthelmintics may diminish within approximately 10 yr of use relying on several factors such as anthelmintic resistance. This study aimed to assess in vitro effect of Balanites aegyptiaca fruits extract on the cuticle of adult worm of Toxocara canis as naturally alternative therapy.

Methods: B. aegyptiaca fruits were procured from the local markets in Aswan, Upper Egypt and authenticated at the Herbarium of National Research Centre. The effect of methanolic extract of B. aegyptiaca fruits on adult T. canis after 24 and 48 h incubating the parasites in Ringer solution containing 240 μg/ml Balanites extract was determined by scanning electron microscopy.

Results: The main changes induced by treatment with the tested extract were wrinkled cuticular surface and deformed sensory papillae. This cuticular distortion would undoubtedly disrupt its protective function and might be enough to expel Toxocara worms from dog’s intestine.

Conclusion: The use of this plant offers a chance for new nematocidal agent, which is economical alternative for the more expensive anthelmintics.

Shalaby HA, Abdel-Shafy S, Abdel-Rahman KA, Derbala AA. Comparative in vitro effect of artemether and albendazole on adult Toxocara canis. Parasitol Res. 2009; 105: 967-976.

Kozan E, Sevimli FK, Birdane FM, Adanir R. Efficacy of eprinomectin against Toxocara canis. Parasitol Res. 2008; 102: 397-400.

Prichard R. Anthelmintic resistance. Vet Parasitol. 1994; 54: 259-268.

Hall JB, Walker DH. Balanites aegyptiaca Del.- A Monograph. School of Agricultural and Forest Science, University of Wales, Banger, 1991, pp. 1-12

Kloos H, McCullough FS. Plant molluscicides. J Med Plant Res. 1987; 46: 195-209.

Shalaby HA, El Namaky AH, Khalil FA, Kandil OM. Efficacy of methanolic extract of Balanites aegyptiaca fruit on Toxocara vitulorum. Vet Parasitol. 2012; 183: 386-392.

Shalaby H, Nasr S, Farag T. Tegumental effects of methanolic extract of Balanites aegyptiaca fruits on adult Paramphistomum microbothrium (Fischoeder 1901) under laboratory conditions. Iran J Parasitol. 2016; 11: 396-405.

Shalaby HA, Abdel-Shafy S, Derbala AA. The role of dogs in transmission of Ascaris lumbricoides for humans. Parasitol Res. 2012; 106: 1021-1026.

Shalaby MA, Moghazy FM, Shalaby HA, Nasr SM. Effect of methanolic extract of Balanites aegyptiaca fruits on enteral and parenteral stages of Trichinella spiralis in rats. Parasitol Res. 2010; 107: 17-25.

Skrjabin KI, Shikhobalova NP, Mozgovoi AA. Oxyurata and Ascaridata. In: Skrjabin KI (ed) Key to parasitic nematoda, vol 2. Brill, Leiden, 1991.

Mehlhorn H, Harder A. Effects of the synergistic action of febantel and pyrantel on the nematode Heterakis spumosa: a light and transmission electron microscopy study. Parasitol Res. 1997; 83: 419-434.

Challam M, Roy B, Tandon V. Effect of Lysimachia ramosa (Primulaceae) on helminth parasites: motility, mortality and scanning electron microscopic observations on surface topography. Vet Parasitol. 2010; 169: 214-218.

Alvarez LL, Mottier ML, Lannsse CE. Drugs transfer into target helminth parasites. Trends Parasitol. 2007; 23: 97-104.

Sparg SG, Light ME, van Staden J. Biological activities and distribution of plant saponins. J Ethnopharmacol. 2004; 94: 219-243.

Gnoula C, Guissou P, Duez P, Frederich M, Dubois J. Nematocidal compounds from the seeds of Balanites aegyptiaca isolation and structure elucidation. Int J Pharmacol. 2007; 3: 280-284.

IssueVol 13 No 4 (2018) QRcode
SectionShort Communication(s)
Toxocara canis Balanites aegyptiaca fruits In vitro effect Cuticle Scanning electron mi-croscopy

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How to Cite
SHALABY H, EL NAMAKY A, KANDIL O, HASSAN N. In Vitro Assessment of Balanites aegyptiaca Fruit Methanolic Extract on the Adult Toxocara canis. Iran J Parasitol. 2018;13(4):643-647.