Performance of Antigen B Isolated from Different Hosts and Cyst Locations in Diagnosis of Cystic Echinococcosis
Abstract
Background: The aim of this study was to assess the performance of Antigen B (AgB) isolated from different Echinococcus granulosus intermediate hosts and from different cyst locations for immunodiagnosis of human cystic echinococcosis (CE).
Methods: Hydatid cyst fluids were collected from lung and liver cysts of sheep, liver cysts of goats, lung cysts of camels and cattle, and liver cysts of human. AgB was purified from each of these hydatid cysts fluids. Serum samples obtained from 47 pathologically confirmed cases of CE along with 30 sera samples from non-CE patients and 40 sera from healthy individuals were tested by ELISA using AgB prepared from different hosts or cyst locations.
Results: The highest sensitivity (97.8%) for diagnosis of CE was seen with AgB prepared from human liver cysts. This maximal sensitivity was followed by AgB isolated from those of sheep liver and lung cysts. The least sensitivity was found with AgB prepared from bovine lung cysts. The highest specificities (97.1%) were observed with AgB isolated from human liver cysts followed by those of sheep and goat liver cysts while the lowest specificity was seen with AgB isolated from bovine lung cysts. In view of the specificities and sensitivities of the different AgB, the best validity was found for AgB prepared from human liver cysts while the least validity was found with AgB prepared from bovine lung cysts.
Conclusion: For any AgB-based tests, obtaining of the antigen from one of these sources will significantly increase the diagnostic sensitivity and specificity of the assay.
Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev. 2004; 17(1):107-135.
Ortona E, Rigano R, Buttari B, Delunardo F, Ioppolo S, Margutti P et al. An update on immunodiagnosis of cystic echinococcosis. Acta Trop. 2003; 85(2):165-171.
Siracusano A, Bruschi F. Cystic echinococcosis: progress and limits in epidemiology and immunodiagnosis. Parasitologia 2006; 48(1-2):65-66.
Carmena D, Benito A, Eraso E. Antigens for the immunodiagnosis of Echinococcus granulosus infection: An update. Acta Trop. 2006; 98(1):74-86.
Sadjjadi SM, Abidi H, Sarkari B, Izadpanah A, Kazemian S. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiagnosis of human hydatidosis. Iran J Immunol. 2007; 4(3):167-172.
Mamuti W, Sako Y, Nakao M, Xiao N, Nakaya K, Ishikawa Y et al. Recent advances in characterization of Echinococcus antigen B. Parasitol Int. 2006; 55:S57-S62.
Leggatt GR, Yang W, McManus DP. Serological evaluation of the 12 kDa subunit of antigen B in Echinococcus granulosus cyst fluid by immunoblot analysis. Trans R Soc Trop Med Hyg., 1992; 86(2):189-192.
de la Rue ML, Yamano K, Almeida CE, Iesbich MP, Fernandes CD, Goto A et al. Serological reactivity of patients with Echinococcus infections (E. granulosus, E. vogeli, and E. multilocularis) against three antigen B subunits. Parasitol Res. 2010; 106(3):741-745.
Sarkari B, Sadjjadi SM, Abidi H, Izadpanah A, Kazemian S, Rafati A. Application of western blotting using native antigen B for serodiagnosis of human cystic echinococcosis. Iran J Parasitol. 2007; 2(3):7-12.
Siracusano A, Buttari B, Delunardo F, Profumo E, Margutti P, Ortona E et al. Critical points in the immunodiagnosis of cystic echinococcosis in humans. Parasitologia 2004; 46(4):401-403.9
Sadjjadi SM, Sedaghat F, Hosseini SV, Sarkari B. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol. 2009; 47(2):153-157.
Ito A, Nakao M, Sako Y. Echinococcosis: serological detection of patients and molecular identification of parasites. Future Microbiol. 2007; 2:439-449.
van Doorn HR, Wentink-Bonnema E, Rentenaar RJ, van GT. Specific crossreactivity in sera from cystic echinococcosis patients in an enzymelinked immunoelectrotransfer blot for cysticercosis diagnostics. Trans R Soc Trop Med Hyg. 2007; 101(9):948-950.
Lorenzo C, Ferreira HB, Monteiro KM, Rosenzvit M, Kamenetzky L, Garcia HH et al. Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study. J Clin Microbiol. 2005; 43(6):2764-2770.
Poretti D, Felleisen E, Grimm F, Pfister M, Teuscher F, Zuercher C et al. Differential immunodiagnosis between cystic hydatid disease and other crossreactive pathologies. Am J Trop Med Hyg. 1999; 60(2):193-198.
McVie A, Ersfeld K, Rogan MT, Craig PS. Expression and immunological characterisation of Echinococcus granulosus recombinant antigen B for IgG4 subclass detection in human cystic echinococcosis. Acta Trop. 1997; 67(1-2):19-35.
Rigano R, Profumo E, Bruschi F, Carulli G, Azzara A, Ioppolo S et al. Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses. Infect Immun. 2001; 69(1):288-296.
Tawfeek GM, Elwakil HS, Awad NS, El-Hoseiny L, Thabet HS, Sarhan RM et al. Genetic variability of antigen B among Echinococcus granulosus Egyptian isolates. Korean J Parasitol. 2009; 47(3):259-264.
Harandi MF, Hobbs RP, Adams PJ, Mobedi I, Morgan-Ryan UM, Thompson RC. Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology 2002; 125:367-373.
Rokni MB. Echinococcosis /hydatidosis in Iran. Iran J Parasitol. 2009; 4(2):1-16.
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| Issue | Vol 6 No 1 (2011) | |
| Section | Articles | |
| Keywords | ||
| Hydatidosis ELISA Serodiagnosis Antigen | ||
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