Intraperitoneal Inoculation: An Atypical Route of Trichinella spiralis Infection
-
Peng JIANG
,
Zi Fang ZHANG
,
Zhong Quan WANG
,
Ruo Dan LIU
,
Xi ZHANG
,
Ge Ge SUN
,
Xin QI
,
Li WANG
,
Jing CUI
Abstract
Background: The intraperitoneal injection is a common method for establishing the experimental animal model infected with parasites. The aim of this study was to investigate if the intraperitoneal injection was another route of Trichinella spiralis infection.
Methods: From June to July 2015, twenty BALB/c mice were intraperitoneally injected with 300 T. spiralis muscle larvae in Department of Parasitology, Medical College, Zhengzhou University, China. The larvae per gr (LPG) muscle from the infected mice and the reproductive capacity index (RCI) of T. spiralis were calculated
Results: Sixty percent (12/20) mice injected were successfully infected at 35 day post injection (dpi), but the muscle larval burden (381.53 larvae per gr) and reproductive capacity index (32.33) in infected mice was lower.
Conclusion: A mechanical force indicated as a possible mechanism in successful larval invasion of almost all kind of host tissues. However, the exact migratory route of larvae from peritoneal cavity into small intestine is not clear.
Gagliardo LF, McVay CS, Appleton JA. Molting, ecdysis, and reproduction of Trichinella spiralis are supported in vitro by intestinal epithelial cells. Infect Immun. 2002; 70(4):1853-59.
Ren HJ, Cui J, Wang ZQ, Liu RD. Normal mouse intestinal epithelial cells as a model for the in vitro invasion of Trichinella spiralis infective larvae. PLoS One. 2011; 6(10): e27010.
Campbell WC. Trichinella and Trichinosis. Plenum Press, NewYork, pp., 1983. 75-151.
Cosoroaba I, Orjanu N. Congenital trichinellosis in the rat. Vet Parasitol. 1998; 77(2-3):147-51.
Dubinský P, Böör A, Kinceková J, Tomasovicová O, Reiterová K, Bielik P. Congenital trichinellosis? Case report. Parasite. 2001; 8(2 Suppl): S180-2.
Boulos LM, Ibrahim IR, Said DE, El-Zawawy LA. Congenital trichinellosis in experimentally infected mice. J Egypt Soc Parasitol. 2005; 35(2): 433-45.
Wranicz MJ, Cabaj W, Moskwa B. Trichinella spiralis: the infectivity of synchronous newborn larvae of different ages inoculated intraocularly. Parasitol Res. 1999; 85(4): 290-92.
Akbar H, Dimier-Poisson I, Moiré N. Role of CD4+ Foxp3+ Regulatory T Cells in Protection Induced by a Live Attenuated, Replicating Type I Vaccine Strain of Toxoplasma gondii. Infect Immun. 2015; 83(9): 3601-11.
Kaur S, Kaur T, Garg N, Mukherjee S, Raina P, Athokpam V. Effect of dose and route of inocu-lation on the generation of CD4+ Th1/Th2 type of immune response in murine visceral leishman-iasis. Parasitol Res. 2008; 103(6):1413-9.
Fischer PU, Curtis KC, Marcos LA, Weil GJ. Molecular characterization of the North American lung fluke Paragonimus kellicotti in Missouri and its development in Mongolian gerbils. Am J Trop Med Hyg. 2011; 84(6):1005-11.
Ahmadnia S, Moazeni M, Mohammadi-Samani S, Oryan A. In vivo evaluation of the efficacy of al-bendazole sulfoxide and albendazole sulfoxide loaded solid lipid nanoparticles against hydatid cyst. Exp Parasitol. 2013; 135(2): 314-9.
Gamble HR, Bessonov AS, Cuperlovic K, Gajadhar AA, van Knapen F, Noeckler K, Sche-none H, Zhu X. International Commission on Trichinellosis: recommendations on methods for the control of Trichinella in domestic and wild an-imals intended for human consumption. Vet Parasitol. 2000; 93(3-4): 393-408.
Li F, Cui J, Wang ZQ, Jiang P. Sensitivity and optimization of artificial digestion in the inspection of meat for Trichinella spiralis. Foodborne Pathog Dis. 2010; 7(8): 879-85.
Murrell KD, Lichtenfels RJ, Zarlenga DS, Pozio E. The systematics of the genus Trichinella with a key to species. Vet Parasitol. 2000; 93(3-4): 293-307.
Zhang YL, Wang ZQ, Li LG, Cui J. Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB/c mice. Parasit Vectors. 2013; 6: 246.
Radovic I, Gruden-Movsesijan A, Ilic N, Mo-starica-Stojkovic M, Sofronic-Milosavljevic L. Trichinella spiralis shares epitopes with human au-toantigens. Mem Inst Oswaldo Cruz. 2012; 107(4): 503-9.
Liu RD, Wang ZQ, Wang L, Long SR, Ren HJ, Cui J. Analysis of differentially expressed genes of Trichinella spiralis larvae activated by bile and cultured with intestinal epithelial cells using real-time PCR. Parasitol Res. 2013; 112(12): 4113-20.
Ren HJ, Cui J, Yang W, Liu RD, Wang ZQ. Identification of differentially expressed genes in Trichinella spiralis larvae after exposure to host in-testine milieu. PLoS One. 2013; 8(6): e67570.
Li LG, Wang ZQ, Liu RD, Yang X, Liu LN, Sun GG, Jiang P, Zhang X, Zhang GY, Cui J. Trichinella spiralis：Low vaccine potential of glutathione S-transferase against infections in mice. Acta Trop. 2015; 146: 25-32.
Liu P, Wang ZQ, Liu RD, Jiang P, Long SR, Liu LN, Zhang XZ, Cheng XC, Yu C, Ren HJ, Cui J. Oral vaccination of mice with Trichinella spiralis nudix hydrolase DNA vaccine delivered by attenuated Salmonella elicited protective immunity. Exp Parasitol. 2015; 153: 29-38.
Ko RC, Fan L, Lee DL, Compton H. Changes in host muscles induced by excretory/secretory products of larval Trichinella spiralis and Trichinella pseudospiralis. Parasitology. 1994; 108 (Pt 2):195-205.
Cui J, Wang ZQ, Han HM. Congenital transmis-sion of Trichinella spiralis in experimentally infected mice. Helminthologia. 2006; 43(1): 7-10.
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| Issue | Vol 12 No 1 (2017) | |
| Section | Short Communication(s) | |
| Keywords | ||
| Trichinella spiralis Infection route Intraperitoneal injec-tion Mechanical force | ||
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