Original Article

Parasitic Burdens, Egg Output, Hemathologic, and Biochemical Changes in Naturally Infected Lambs with Dicrocoelium dendriticum


Background: We investigated the variations of heamathologic and biochemical parameters with different parasitic burdens and the correlation between fecal egg counts and fluke number in livers of sheep naturally infected with Dicrocoelium dendriticum.

Methods: In order to detect excretion of D. dendriticum eggs, fecal samples from 120 sheep of different age groups were collected individually at slaughterhouse in Neyshabur County in Razavi Khorasan Province during December 2013 - October 2014. Hematologic and blood biochemical values in 120 sheep naturally infected with D. dendriticum were compared with equivalent values in 120 parasite-free sheep from an organically farmed flock. Investigated animals were kept in outdoor system, on pastures covered with swamps, which remain flooded after rainy season.

Results: Total numbers of flukes in livers obtained from infected sheep at autopsy varied between 10 and 18,500. A positive relationship was noted (r= 0.94, P < 0.001) between the number of eggs excreted by each sheep and that of total fluke counts in the liver. Significantly, lower red blood cell, packed cell volume, neutrophil and albumin were observed in parasitized sheep, when compared to the healthy animals. White blood cells,, lymphocyte and aspartate aminotransferase values were significantly higher in parasitized sheep. No significant correlation was detected between the hematologic and biochemical parameters and the number of D. dendriticum in the liver.

Conclusion: Infection with > 400 D. dendriticum in the liver could be diagnosed by egg per gram. However, there was no relationship between the parasite burden and hematologic and biochemical parameters.

Otranto D, Traversa D. Dicrocoeliosis of ruminants: a little known fluke disease. Trends Parasitol. 2003;19,12-15.

Borji H, Parandeh S. The abattoir condemnation of meat because of parasitic infection, and its economic importance: results of a retrospective study in northeastern Iran. Ann Trop Med Parasitol. 2010;104(8):641-7.

Borji H, Azizzadeh M, Kameli M. A retrospective study of abattoir condemnation due to parasitic infections and its economic importance in Ahwaz, southwestern of Iran. J Parasitol. 2012; 98(5), 954–957.

Otranto D, Traversa D. A review of dicrocoeliosis of ruminants including recent advances in the diagnosis and treatment. Vet Parasitol. 2002; 107, 317-335.

Manga-Gonzalez MY, Gonz‡lez-Lanza C, Del-Pozo-Carnero P. Dynamics of the elimination of Dicrocoelium dendriticum (Trematoda, Digenea) eggs in the faeces of lambs and ewes in Porma basin (Leon, NW Spain). Ann Parasitol Hum Comp.1991; 66, 57-61.

Foix J .Traitement de la dicrocoeliose par le cambendazole z. Rev. Med. Vet.1977; 128, 1111-1119.

Dorchies P, Ducos de Lahitte J, Alzieu J.P, Bichet H. Essai de traitement de la dicrocoeliose du mouton par le thiophanate. Rev Med Vet. 1988; 139, 277-279.

Calamel M, Giauffret A. Interpretation des resultats dans le diagnostic coproscopique de la dicrocoeliose. Res Med Vet. 1976;152, 99-104.

Campo R, Manga-Gonz‡lez M.Y, Gonz‡lez-Lanza C. Relationship between egg output and parasitic burden in lambs experimentally infected with different doses of Dicrocoelium dendriticum (Digenea). Vet Parasitol. 2000; 87, 139-149.

Rehbein S, Lindner T, Kokott S. Dicrocoelium dendriticum infection in sheep: faecal egg count, gall bladder egg count, gall bladder fluke count, total fluke count. Helminthologia. 2002; 2, 71-75.

Sanz F, Tarazona JM, Jurado R, Frıas J, Tarazona JV, Duncan JL. An evaluation of the efficacy of netobimin against Dicrocoelium dendriticum in sheep. Vet Rec.1987;120: 57–58.

Rojo-Vazquez F, Cordero del Campillo M, Diez-Banos P, Chaton-Schaffner M, Vazquez F.A.R, Banos P.D, Schaffner M.C. Relation existant entre le nombre dÕoeufs dans les feces et la charge parasitaire lors des infestations naturelles a Dicrocoelium dentriticum chez les ovins. Rev Med. Vet.1981;132, 601-607.

Senlük B, Irak V, Muz M, Tinar R. Changes in Faecal Egg Counts at Different Hours of the Day and Relationship between Faecal Egg Count and Parasite Burden in Sheep Naturally Infected with Dicrocoelium dendriticum. Turk J Vet Anim Sci. 2005; 30, 107-111.

Chandra G. First detection of Dicrocoelium eggs in the faeces of experimentally infected lambs, guinea pigs and pigs. HAU J Res Hissar.1973; 3, 31–34.

Tarry DW. Dicrocoelium dendriticum, the life cycle in Britain. J Helminthol.1969; 43, 403–416.

Mapes C.R. Cionella lubrica (Müller), a new intermediate host of Dicrocoelium dendriticum (Rudolphi,1819) Looss (1899) (Trematoda, Dicrocoeliidae). J Parasit.1952; 38, 84.

Wolff K, Hauser B, Wild P. [Dicrocoeliasis of sheep: studies of the pathogenesis and regeneration of the liver following therapy]. Berl Munch Tierarztl Wochenschr.1984; 97:378–387.

Ranucci S, Mughetti L, Ambrosi M, Grelloni V. Rilievi e-matochimici, anatomo-istopatologici e parassitologici in ovine infetti da dicroceliosi. Riv Zoot Vet.1981;9,173–175.

Scala A, Coda S, Ximenes LA et al. Dicroceliasi ovina in Sardegna: Tassi di prevalenza, lesioni anatomo-istopatologiche e tests ematochimici. Atti Fe Me S P Rum.1991;1,209–214.

Manga-Gonzalez MY, Ferreras MC, Campo R, Gonzalez-Lanza C, Perez V, Garcıa-Marın J.F. Hepatic marker enzymes, biochemical parameters and pathological effects in lambs experimentally infected with Dicrocoelium dendriticum (Digenea). Parasitol Res.2004; 93, 344–355.

Theodoridis Y, Duncan J.L, MacLean J.M, Himonas C.A. Pathophysiological studies on Dicrocoelium dendriticumin fection in sheep. Vet Parasitol.1991;39, 61 – 66.

IssueVol 11 No 3 (2016) QRcode
SectionOriginal Article(s)
Dicrocoelium dendriticum Parasitic burdens Fecal egg count Hematologic Biochemical

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How to Cite
SAMADIE H, MOHAMMADI G, HEIDARPOUR M, AZIZZADEH M, MALEKI M, BORJI H. Parasitic Burdens, Egg Output, Hemathologic, and Biochemical Changes in Naturally Infected Lambs with Dicrocoelium dendriticum. Iran J Parasitol. 2016;11(3):358-363.