Microsporidial Spores in Fecal Samples of Some Domesticated Animals Living in Giza, Egypt
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Abstract
Background: The aim of the present work was to investigate the prevalence and species of intestinal microsporidiosis among animals in Giza, Egypt.
Methods: A total of 869 animal fecal samples were collected from domesticated animals (dogs, cats, rabbits, cattle, buffaloes, sheep, goats, donkeys and pigs) living in Giza, Egypt. Spores of microsporidia were concentrated from collected samples by centrifugation and finally stained with modified trichrome (MT) stain to detect microsporidial spores. Microsporidial spores in microscopically-positive samples were molecularly confirmed and identified using species-specific primers.
Results: Spores of microsporidia were microscopically detected in 17.0% of the examined animal fecal samples. The highest and lowest rates of infection with intestinal microsporidia were recorded in dogs (33.3%) and buffaloes (6.9%), respectively. Molecularly, the obtained microsporidial spores were classified as Enterocytozoon bieneusi and E. intestinalis. Dual infection with both identified species was observed in fecal samples from buffalo, rabbit, goat, cat, pig and dog.
Conclusion: Domestic animals may play a role in dissemination of intestinal microsporidiosis in the environment. Examined animals were infected with E. bieneusi in a higher percentage than E. intestinalis.
Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, Didier ES, Fan L, Heiman DI, Levin JZ, Young S, Zeng Q, Troemel ER. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. Genome Res. 2012; 22: 2478-88.
Vavra J, Larsson JIR. Structure of the microsporidia. In: Wittner M. (editor). The microsporidia and microsporidiosis. Washington DC: ASM Press, 1999; pp. 7–84.
Mathis A, Weber R, Deplazes P. Zoonotic potential of the microsporidia. Clin Microbiol Rev. 2005; 18: 423-445.
Wittner M. Historic perspective on the microsporidia: expanding horizons. In: Wittner M, Weiss L, (editors.). The microsporidia and microsporidiosis. Washington DC: ASM Press, 1999; pp. 1–6.
Canning EU, Lom J. The Microsporidia of vertebrates. New York: Academic press, 1986; pp. 289.
Kotler DP, Orenstein JM. Clinical syndromes associated with microsporidiosis. Adv Parasitol. 1998; 40: 321–349.
Kotler D, Orenstein JM. Clinical syndromes associated with microsporidiosis. In: Wittner, M., Weiss, L. (editors.). The microsporidia and microsporidiosis. Washington DC: ASM press, 1999; pp. 258–292.
Weber R, Deplazes P, Schwartz D. Diagnosis and clinical aspects of human microsporidiosis. Contrib Microbiol. 2000; 6: 166–192.
Thomarat F, Vivares CP, Gouy M. Phylogenetic analysis of the complete genome sequence of Encephalitozoon cuniculi supports the fungal origin of microsporidia and reveals a high frequency of fast-evolving genes. J Mol Evol. 2004; 59: 780–791.
Keeling PJ, Fast NM. Microsporidia: biology and evolution of highly reduced intracellular parasites. Annu Rev Microbiol. 2002; 56: 93–116.
Didier ES, Weiss LM. Microsporidiosis: current status. Curr Opin Infect Dis. 2006; 19: 485–492.
Didier ES, Stovall ME, Green LC, Brindley PJ, Sestak K, Didier PJ. Epidemiology of microsporidiosis: sources and modes of transmission. Vet Parasitol. 2004; 126: 145–166.
Snowden KF, Didier ES, Orenstein JM, Shadduck JA. Animal models of human microsporidial infections. Lab Anim Sci. 1998; 48: 589–592.
Snowden KF, Shadduck JA. Microsporidia of higher vertebrates. In: Wittner, M., Weiss, L. (editors.). The microsporidia and microsporidiosis. Washington, DC; ASM press. 1999; pp. 393–419.
Deplazes P, Mathis A, Weber R. Epidemiology and zoonotic aspects of microsporidia of mammals and birds. Contrib Microbiol. 2000; 6: 236–260.
Hutin YJ, Sombardier MN, Liguory O, Sarfati C, Derouin F, Modai J, Molina JM. Risk factors for intestinal microsporidiosis in patients with human immunodeficiency virus infection: a case-control study. J Infect Dis. 1998; 178: 904–907.
Dascomb K, Frazer T, Clark RA, Kissinger P, Didier E. Microsporidiosis and HIV. J Acquir Immune Defic Syndr. 2000; 24: 290–292.
USEPA. Contaminant Candidate List – CCL1. 1998. http://water.epa.gov/scitech/drinkingwater/dws/ccl/ccl1.cfm#list.
Galván-Díaz AL, Magnet A, Fenoy S, Hen-riques-Gil N, Haro M, Gordo FP, Miró G, del Águila C, and Izquierdo F. Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain. PLoS One. 2014; 9: e92289.
Lobo ML, Xiao L, Cama V, Magalhaes N, Antunes F, Matos O. Identification of potentially human-pathogenic Enterocytozoon bieneusi genotypes in various birds. Appl Environ Microbiol. 2006; 72: 7380–7382.
Mori H, Mahittikorn A, Thammasonthijarern N, Chaisiri K, Rojekittikhun W, Sukthana Y. Presence of zoonotic Enterocytozoon bieneusi in cats in a temple in central Thailand. Vet Parasitol. 2013; 197: 696-701.
Sak B, Petrzelkova KJ, Kvetonova D, My-narova A, Shutt KA, Pomajbikova K, Ka-lousova B, Modry D, Benavides J, Todd A, Kvac M. Long-term monitoring of microsporidia, Cryptosporidium and Giardia infections in western Lowland Gorillas (Gorilla gorilla gorilla) at different stages of habituation in Dzanga Sangha Protected Areas, Central African Republic. PLoS One. 2013; 8(8):e71840.
Ye J, Xiao L, Li J, Huang W, Amer SE, Guo Y, Roellig D, Feng Y. Occurrence of human-pathogenic Enterocytozoon bieneusi, Giardia duodenalis and Cryptosporidium genotypes in laboratory macaques in Guangxi, China. Parasitol Int. 2014; 63: 132-137.
24- Li W; Diao R, Yang J, Xiao L, Lu Y, Li Y, Song M. High diversity of human-pathogenic Enterocytozoon bieneusi genotypes in Swine in northeast China. Parasitol Res. 2014; 113(3): 1174-53.
Bern C, Hernandez B, Lopez MB, Arrowood MJ, de Mejia MA, de Merida AM, Hightower AW, Venczel L, Herwaldt BL, Klein RE. Epidemiologic study of Cyclospora cayetanensis in Guatemala. Emerg Infect Dis. 1999; 5: 766-774.
Weber R, Bryan RT, Owen RL, Wilcox CM, Gorelkin L, Visvesvara GS. Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates. N Engl J Med. 1992; 326: 161-166.
Fedorko DP, Nelson NA, Cartwright CP. Identification of microsporidia in stool specimens by using PCR and restriction endonucleases. J Clin Microbiol. 1995; 33: 1739–1741.
Da Silva AJ, Schwartz DA, Visvesvara GS, Moura H, Slemenda SB, Pieniazek NJ. Sensitive PCR diagnosis of infections by Enterocytozoon bieneusi (Microsporidia) using primers based on the region coding for small-subunit rRNA. J Clin Microbiol. 1996; 34: 986-987.
Da Silva AJ, Slemenda SB, Visvesvara GS, Schwartz DA, Wilcox CM, Wallace S, Pieniazek NJ. Detection of Septata intestinalis (Microsporidia), Cali et al. 1993, using polymerase chain reaction primers targeting the small subunit ribosomal RNA coding region. Mol Diagn. 1997; 2: 47–52.
Katzwinkel-Wladarsch S, Deplazes P, Weber R, Löscher T, Rinder H. Comparison of polymerase chain reaction with light microscopy for detection of microsporidia in clinical specimens. Eur J Clin Microbiol Infect Dis. 1997; 16: 7-10.
Lobo ML, Teles A, da Cunha MB, Henriques J, Lourenço AM, Antunes F, Matos O. Microsporidia detection in stools from pets and animals from the zoo in Portugal: a preliminary study. J Eukaryot Microbiol. 2003; 50: 581-582.
Lores B, del Aguila C, Arias C. Enterocytozoon bieneusi (microsporidia) in fecal samples from domestic animals from Galicia, Spain. Mem Inst Oswaldo Cruz. 2002; 97: 941-945.
Bornay-Linares FJ, da Silva AJ, Moura H, Schwartz DA, Visvesvara GS, Pieniazek NJ, Cruz-Lopez A, Hernandez-Jauregui P, Guerrero J, Enriquez FJ. Immunologic, microscopic, and molecular evidence of Encephalitozoon intestinalis (Septata intestinalis) infection in mammals other than humans. J Infect Dis. 1998; 178: 820-826.
Dengjel B, Zahler M, Hermanns W, Heinritzi K, Spillmann T, Thomschke A, Löscher T, Gothe R, Rinder H. Zoonotic potential of Enterocytozoon bieneusi. J Clin Microbiol. 2001; 39: 4495-4499.
Sulaiman IM, Fayer R, Yang C, Santin M, Matos O, Xiao L. Molecular characterization of Enterocytozoon bieneusi in cattle indicates that only some isolates have zoonotic potential. Parasitol Res. 2004; 92: 328-334.
Lee JH. Molecular detection of Enterocytozoon bieneusi and identification of a potentially human-pathogenic genotype in milk. Appl Environ Microbiol. 2008; 74: 1664-1666.
Del Coco VF, Córdoba MA, Bilbao G, de Almeida Castro P, Basualdo JA, Santín M First report of Enterocytozoon bieneusi from dairy cattle in Argentina. Vet Parasitol. 2014; 199: 112-115.
Sak B, Kváč M, Hanzlíková D, Vitaliano C. First report of Enterocytozoon bieneusi infection on a pig farm in the Czech Republic. Vet Parasitol. 2008; 153: 220-224.
Valencáková A, Balent P, Húska M, Novotný F, Luptáková L. First report on Encephalitozoon intestinalis infection of swine in Europe. Acta Vet Hung. 2006; 54: 407-411.
Breitenmoser AC, Mathis A, Bürgi E, Weber R, Deplazes P. High prevalence of Enterocytozoon bieneusi in swine with four genotypes that differ from those identified in humans. Parasitology. 1999; 118: 447-453.
Buckholt MA, Lee JH, Tzipori S. Prevalence of Enterocytozoon bieneusi in swine: an 18-month survey at a slaughter house in Massachusetts. Appl Environ Microbiol. 2002; 68: 2595-2599.
Abe N, Kimata I, Iseki M. Molecular evidence of Enterocytozoon bieneusi in Japan. J Vet Med Sci. 2009; 71: 217-219.
Cama V, Cabrera L, Lopera C, Vargas M, Taquiri C, Smit H, Xiao L. Molecular characterization of Cryptosporidium and microsporidia from goats and sheep in Peru, abstr. 10th International workshops on opportunistic protists, Boston, MA 2008; PL3.
Del Aguila C, Izquierdo F, Navajas R, Pieniazek NJ, Miró G, Alonso AI, Da Silva AJ, Fenoy S. Enterocytozoon bieneusi in animals: rabbits and dogs as new hosts. J Eukaryot Microbiol. 1999; 46: 8S-9S.
Santín M, Trout JM, Vecino JA, Dubey JP, Fayer R. Cryptosporidium, Giardia and Enterocytozoon bieneusi in cats from Bogota (Colombia) and genotyping of isolates. Vet Parasitol. 2006; 141: 334-339.
Santín M, Cortés Vecino JA, Fayer R. Ente-rocytozoon bieneusi genotypes in dogs in Bogota, Colombia. Am J Trop Med Hyg. 2008; 79: 215–217.
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| Issue | Vol 11 No 2 (2016) | |
| Section | Original Article(s) | |
| Keywords | ||
| Intestinal microsporidia Modified trichrome PCR Domesticated animals Fecal samples | ||
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