Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein

Abstract

Background: Cryptosporidium parvum is a parasitic protozoan that functions as important causa­tive agent of diarrhea in human and animals. The host's immune response to surface anti­gens of C. parvum has been previously demonstrated. In this respect, the role of humoral immu­nity in the development of host protective immunity against this protozoon has been well demon­strated.

Methods: The effect of specific chicken egg yolk antibody (IgY) against recombinant C. parvum P23 was examined. IgY sample was prepared from eggs of chickens immunized with recombi­nant C. parvum protein p23 and analyzed with C. parvum lysate and recombinant P23.

Results: The anti P23 specific IgY was recognized a protein band with approximately 23 kDa in lys­ates prepared from the C. parvum oocysts. Also dot blot analysis of recombinant P23 showed that it could be recognized by the anti P23 specific IgY up to 1/1000 dilution of antibody. But the best antibody dilution for immunological studies was determined as 1:200.

Conclusion: Since P23 is an immunodominant surface glycoprotein expressed in the early phase of infection, specific IgY against recombinant p23 could be recommended as a favorable candi­date for passive immunization against C. parvum infection in human and animals.