In Vitro Susceptibility of Iranian Isolates of Trichomonas vaginalis to Metronidazole
-
Mohammad MATINI
,
Amir-Hossein MAGHSOOD
,
Mahdi MOHEBALI
,
Soghra RABIEE
,
Mohammad FALLAH
,
Sassan REZAIE
,
Mostafa REZAEIAN
Abstract
Background: Metronidazole, a 5-nitroimidazole derivative, is the main antitrichomonal agent of choice for treatment of trichomoniasis. Since 1962, some cases of treatment failure with metronidazole have been reported and recently drug resistance is now on the rise in the world. This study was aimed to determine current susceptibility of Iranian isolates of Trichomonas vaginalis to metronidazole.
Methods: This study was performed on 50 T. vaginalis isolates collected from west and central areas of Iran. After axenisation of the parasites, susceptibility testing was carried out by using serial twofold dilutions of metronidazole (400 to 0.1 µg/ml). The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the trichomonads were determined after 48 h incubation at 35.5 °C. Drug susceptibility assays of the all isolates were carried out two times in triplicate under aerobic and anaerobic conditions.
Results: Ninety-eight percent of the T. vaginalis isolates (49/50) were sensitive to metronidazole. Metronidazole resistance was defined as aerobic MIC ≥50 µg/ml, detected in one isolate. The means of aerobic MICs and MLCs and that of anaerobic MICs of the parasites were 2.91, 1.95 and 0.28 µg/ml, respectively.
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| Issue | Vol 11 No 1 (2016) | |
| Section | Original Article(s) | |
| Keywords | ||
| In vitro Iran Metronidazole Parasitic sensitivity tests Trichomonas vaginalis | ||
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