Genetic Characterization of Toxoplasma gondii from Zoo Wild¬life and Pet Birds in Fujian, China
AbstractBackground: Toxoplasmosis, a worldwide zoonotic disease, is caused by Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild animals is of great importance to understand the transmission of the parasite in the environment. However, little is known about T. gondii prevalence in wild animals and birds in China.Methods: We conducted the genetic characterization of T. gondii isolated from Zoo Wild Animals and Pet Birds in Fujian Province, Southeastern China. Heart tissues were collected from 45 zoo animals and 140 pet birds. After identified using B1 gene, the genetic diversity of T. gondii isolates were typed at 11 genetic markers, including SAG1,5’ and3’-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3.Results: Seven of 45 zoo animals and 3 of 140 pet birds were positive by PCR amplification using T. gondii B1 gene specific primers. Of these positive isolates, 3 isolates from Black-capped (Cebus apella), Peacock (Peafowl) and Budgerigar (Melopsittacus undulatus) were successfully genotyped at 11 genetic loci, and grouped to three distinct genotypes: ToxoDB Genotype #9, #2 and #10, respectively.Conclusion: This is the first genotyping of T. gondii isolated from zoo wild animals and pet birds in Fujian, China. There is a potential risk for the transmission of this parasite through zoo wild animals and pet birds in this region.
Dubey JP. Toxoplasmosis of animals and hu-mans. 2nd, CRC Press Inc, Boca Raton, New York. 2010;1-313
Rajendran C, Su C, Dubey JP. Molecular geno-typing of Toxoplasma gondii from Central and South America revealed high diversity within and between populations. Infect Genet Evol. 2012;12(2): 359-368.
Weiss LM, Dubey JP. Toxoplasmosis: A his-tory of clinical observations. Int J Parasitol. 2009;39(8): 895-901.
Salant H, Weingram T, Spira DT, Eizenberg T. An outbreak of Toxoplasmosis amongst squir-rel monkeys in an Israeli monkey colony. Vet Parasitol. 2009;159(1): 24-29.
Murata K. A serological survey of Toxoplasma gondii infection in zoo animals and other ani-mals. Jpn J Vet Sci. 1989;51(5): 935-940.
Zhang SY, Wei MX, Zhou ZY, Yu JY, Shi XQ. Prevalence of antibodies to Toxoplasma gondii in the sera of rare wildlife in the Shanghai Zoo-logical Garden, People’s Republic of China. Parasitol Int. 2000;49(2): 171-174.
Silvia de Camps, Dubey JP, Saville WJA. Seroepidemiology of Toxoplasma gondii in Zoo Animals in Selected Zoos in the Midwestern United States. J Parasitol. 2008;94(3): 648-653.
Gennari SM, Ogrzewalska M, Soares HS, Saraiva DG, PinterA, Labruna MB, Dubey JP. Occurrence of Toxoplasma gondii antibodies in birds from the Atlantic Forest, state of São Paulo, Brazil. Vet Parasitol. 2014;200(1): 193-197.
Cong W, Huang S Y, Zhou DH, Zhang XX, Zhang NZ, Zhao Q, Zhu XQ. Prevalence and genetic characterization of Toxoplasma gondii in house sparrows (Passer domesticus) in Lanzhou, China. Korean J Parasitol. 2013;51(3): 363-367
Howe DK, Honoré S, Derouin F, Sibley D. Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxo-plasmosis. J Clin Microbiol. 1997;35(6): 1411-1414.
Grigg ME, Ganatra J, Boothroyd JC, Margolls TP. Unusual Abundance of Atypical Strains Associated with Human Ocular Toxoplasmo-sis. J Infect Dis. 2001;184(5): 633-639.
Khan A, Su C, German M, Storch GA, Clifford DB, Sibley D. Genotyping of Toxo-plasma gondii Strains from Immunocompro-mised Patients Reveals High Prevalence of Type I Strains. J Clin Microbiol. 2005;43(12): 5881-5887.
Su C, Zhang X, Dubey JP. Genotyping of Tox-oplasma gondii by multilocus PCR-RFLP mark-ers: A high resolution and simple method for identification of parasites. Int J Parasitol. 2006; 36(7): 841-848.
Su C, Shwab EK, Zhou P, Zhu XQ, Dubey JP. Moving towards an integrated approach to mo-lecular detection and identification of Toxo-plasma gondii. Parasitol. 2010;137(01): 1–11.
Dubey JP, Zhu XQ, Sundar N, Zhang H, Kwok OC, Su C. Genetic and biologic charac-terization of Toxoplasma gondii isolates of cats from China. Vet Parasitol. 2007;145(3): 352-356.
Jiang HH, Qin SY, Wang W, He B, Hu TS, Wu JM, Fan QS, Tu CC, Liu Q, Zhu XQ. Prevalence and genetic characterization of Tox-oplasma gondii infection in bats in southern Chi-na. Vet Parasitol. 2014; 203(3): 318-321.
Zhou P, Zhang H, Lin RQ, DL, Song HQ, Su C, Zhu XQ. Genetic characterization of Toxo-plasma gondii isolates from China. Parasitol Int. 2009; 58(2): 193-195.
Huang SY, Cong W, Zhou P, Zhou DH, Wu SM, Xu MJ, Zou FC, Song HQ, Zhu XQ. First report of genotyping of Toxoplasma gondii iso-lates from wild birds in China. J Parasitol. 2012;98(3): 681-682.
Tian YM, Huang SY, Miao Q, Jiang HH, Yang JF, Su C, Zhu XQ, Zou FC. Genetic character-ization of Toxoplasma gondii from cats in Yun-nan Province, Southwestern China. Parasit Vectors. 2014;7: 178.
Burg JL, Grover CM, Pouletty P, Boothroyd JC. Direct and sensitive detection of a patho-genic protozoan, Toxoplasma gondii, by polymer-ase chain reaction. J Clin Microbiol. 1989;27(8): 1787-1792.
Jones CD, Okhravi N, Adamson P, Tasker S, Lightman S. Comparison of PCR detection methods for B1, P30, and 18S rDNA genes of Toxoplasma gondii in aqueous humor. Invest Ophth Vis Sci. 2000;41(3): 634-644.
Pena HF, Gennari SM, Dubey JP, Su C. Popu-lation structure and mouse-virulence of Tox-oplasma gondii in Brazil. Int J Parasitol. 2008; 38(5): 561-569.
Buchbinder S, Blatz R, Rodloff AC. Compari-son of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii. Diagn Micr Infect Dis. 2003;45(4): 269-271.
Switaj K, Master A, Skrzypczak M, Zab-orowski P. Recent trends in molecular diagnos-tics for Toxoplasma gondii infections. Clin Micro-biol Infect. 2005;11(3): 170-176.
Gutierrez J, O’Donovan J, Williams E, Proctor A, Brady C, Marques PX. Detection and quan-tification of Toxoplasma gondii in ovine maternal
and foetal tissues from experimentally infected pregnant ewes using real-time PCR. Vet Parasi-tol. 2010;172(1): 8-15.
Quan JH, Kim TY, Choi IU, Lee YH. Geno-typing of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis. Korean J Parasitol. 2008;46(2): 105-108.
Kompalic-Cristo A, Frotta C, Suárez-Mutis M, Fernandes O, Britto C. Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood. Parasitol Res. 2007;101(3): 619-625
Putignani L, Mancinelli L, Del Chierico F, Menichella D, Adlerstein D, Angelici MC, Ma-rangi M, Berrilli F, Caffara M, di Regalbono DA, Giangaspero A. Investigation of Toxo-plasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent am-plicon generation assay (FLAG). Exp Parasitol. 2011;127(2): 409-417.
Zhao DY, Huan CQ, Wang K, Wen FL, Yue LP, Wang SK, Lin X.Comparison on Toto-plasma gondii detection by triple SYBR Green I real-time PCR and ELISA. Chinese J Zoono-ses. 2013; 29(5): 476-481. (In Chinese)
Zhou P, Nie H, Zhang LX, Wang HY, Yin CC, Su C, Zhu XQ, Zhao JL. Genetic characteriza-tion of Toxoplasma gondii isolates from pigs in China. J Parasitol. 2010;96(5): 1027-1029.
Zhou P, Sun XT, Yin CC, Yang JF, Yuan ZG, Yan HK, Zhu XQ, Zou FC. Genetic charac-terization of Toxoplasma gondii isolates from pigs in southwestern China. J Parasitol. 2011; 97(6): 1193-1195.
Chen ZW, Gao JM, Huo XX, Wang L, Yu L, Halm-Lai F, Xu YH, Song WJ, Hide G, Shen JL, Lun ZR. Genotyping of Toxoplasma gondii isolates from cats in different geographic re-gions of China. Vet Parasitol. 2011;183(1): 166-170.
Velmurugan GV, Dubey JP, Su C. Genotyping studies of Toxoplasma gondii isolates from Africa revealed that the archetypal clonal lineages pre-dominate as in North America and Europe. Vet Parasitol. 2008;155(3): 314-318.