Description of Pallisentis (Brevitritospinus) punctati n. sp. (Acantho¬cephala: Quadrigyridae) from Channa punctatus in Ba¬reilly, Uttar Pradesh, India

  • Neelima GUPTA Centre of Excellence Laboratory, Department of Animal Science, M.J.P. Rohilkhand University, Bareilly-243 006,U.P., India
  • Dileep K. GUPTA Fisheries and Aquaculture Laboratory, Department of Zoology, Bareilly College, Bareilly-243 006, U.P., India
  • Priyanka SINGHAL Centre of Excellence Laboratory, Department of Animal Science, M.J.P. Rohilkhand University, Bareilly-243 006,U.P., India
Keywords: Acanthocephala, Light microscopy, Scanning electron Micros-copy, Bareilly, India


Background: The genus Pallisentis is an endoparasitic acanthocephalan inhabit­ing the intestinal walls. Hooks and spines of the worm are significant taxonomical and adaptive tools.Methods: The parasites were fixed, dehydrated and examined under Olym­pus BX 53 Microscope with DIC attachment, digital camera and CELLSENS imag­ing system [Light microscopy (LM)] and fixed in 2% glutaraldehyde in 0.1 M cacodylate buffer, dehydrated, rotary-coated with gold palladium in NeoCoater 100-240V and examined in Neo JCM-6000 [scanning electron microscopy (SEM)].Results: P. punctati n. sp. (prevalence 65.51%; mean intensity 3-6 par/host) is de­scribed. Females almost twice as large as males; proboscis hooks small; collar spine rows similar [16] and constant in both sexes but number of spines per row greater in females [22] than males [14]; trunk spine rows 28-39 (spines per row 14-18) in females and 20-26 (spines per row 10-12) in males. spine length of females almost twice as long as males, spines extend up to posterior testis in males and ¾ of total body length in females, Saefftigen’s pouch present, nuclei in cement gland 10-11, seminal vesicle, bursa and egg size small. SEM indicated lack of micro sculptures, and spines embedded on pre-trunk and trunk. Sex-based differences apparent (hook sizes, greater number of spines per row and longer spines in pre-trunk and trunk of females). Male trunk spine was narrower and of lateral spine with characteristic hooked appearance.Conclusion: A new species of Pallisentis based on LM and SEM is described, sexual diversity in hook and spine structure is reported.


Golvan YJ. Le phylum de Acanthocephala. Deux-ieme note. Le classe de Eoacanthocephala (Van Cleave, 1936). Ann de Parasitol Hum de Comp. 1959; 34: 5-52.

Golvan YJ. Nomenclature of the acanthocephala. 1994; Res Rev Parasitol. 54: 135–205.

Yamaguti S. Systema Helminthum. Vol. 5, Acantho-cephala. Ist. ed. Wiley Interscience Publishers, New York, London 1963; 423 p.

Tadros G. On three new acanthocephala of the gen-era Pallisentis Van Cleave, Saceosentis gen. n. and Acan-thocephalus Kuelreuther, from fish. J Helminthol. 1966; 40: 155-180.

Gupta V, Sinha G. Check list of acanthocephalan parasites of fishes of India and Pakistan. Indian J Helminthol. 1990; 42: 41-66.

Gupta SP, Verma SL. On three new acanthocephala parasites of the genus Pallisentis Van Cleave, 1928 from freshwater fishes of Lucknow. Helminthologia 1980; 17: 269-282.

Soota TD, Bhattacharya SB. On the validity of the species of the genus Pallisentis Van Cleave, 1928 (Acanthocephala: Pallisentidae) from the Indian sub-continent. Rec Zool Surv India 1982; 80: 157-167.

Gupta V, Fatma S. On some acanthocephalan para-sites (Family: Quadrigyridae Van Cleave, 1920) from fishes of Uttar Pradesh and Tamil Nadu. Indian J Helminthol. 1985; 37: 149-180.

Chowhan JS, Gupta NK, Khera S. On two species of the genus Pallisentis Van Cleave, 1928 from fishes of Gobindsagar Lake and synonymy of the genus Saccosentis Tadros, 1966. Res. Bull Panjab Univ Sci. 1987; 38: 21-26.

Bhattacharya SB. Handbook on Indian Acantho-cephala. Kolkata. Zool. Surv. India. 2007; 255 pp.

Amin OM, Heckmann RA, Van Ha N, Van Luc P, Doanh PN. Revision of the genus Pallisentis (Acan-thocephala: Quadrigyridae) with the erection of three new subgenera, the description of Pallisentis (Brevitri-tospinus) vietnamensis subgen. et sp. n., a key to species of Pallisentis, and the description of a new Quadrigyrid genus, Pararaosentis gen. n. Comp Parasitol. 2000; 67: 40-50.

Amin OM. Classification of the acanthocephala Folia Parasitol. 2013; 60 (4): 273–305.

Gibbons L. SEM guide to the morphology of Nem-atode parasites of vertebrates. CAB International, ISBN 085198-569-6, OxU, United Kingdom 1986.

Gonzalez CE, Hamann MI, Salgado C. Study of helminthes parasites of amphibians by Scanning Electron Microscopy. In: Viacheslav Kazmiruk (ed) Scanning Electron Microscopy, ISBN: 978-953-51-0092-8, Intec, Available from: microscopy/study-of-helminth-parasites-of-amphibians-by-scanning-electron-microscopy 2012.

Huffman DG, Bullock WL. Meristograms: Graphical analysis of serial variation of pro-boscis hooks of Echinorhynchus (Acanthocephala). Syst Zool. 1975; 24: 333–345.

Poulin R. Investing in attachment: Evolution of an-choring structures in acanthocephalan parasites. Biol J Linn Soc. 2007; 90 (4): 637–645.

Bush AO, Lafferty KD, Lotz JM, Shastak AW. Para-sitology meets ecology on its own terms: Margolis et al. revisited. J Parasitol. 1997; 83: 575-583.

Dobbs GH. A method for preservation of soft tissues of marine teleosts for scanning electron microscopy. Tissue Cell. 1972; 4: 687–690.

Gupta R, Maurya R, Saxena AM. Two new species of the genus Pallisentis Van Cleave, 1928 (Acan-thocephala: Quadrigyridae) from the intestine of Channa punctatus (Bloch, 1793) from the River Gomti at Lucknow, India. Iran J Parasitol. 2015;10(1): 116-21.

Huffman DG, Nickol BB. Meristogram analy-sis of the acanthocephalan genus Pompho-rhynchus in North America. J Parasitol. 1979; 65: 851–859.

Bush AO, Fernandez JC, Esch GW, Seed JR. Para-sitism: the diversity and ecology of animal parasites. Cambridge University Press. Cambridge. United Kingdom. 2001; pp 1- 566.

Aznar FJ, Bush AO, Raga JA. Reduction and variability of trunk spines in the acanthoceph-alan Corynosoma cetaceum: The role of physical constraints on attachment. Invert Biol. 2002; 121:104–114.

Dezfuli BS, Lui A, Giari L, Boldrini P, Gio-vinazzo G. Ultra structural study on the body sur-face of the Acanthocephalan parasite Denti-truncus truttae in brown trout. Microsc Res Tech. 2008; 71: 230–235.

Hernández-Orts JS, Timi JT, Raga JA, García-Varela M, Crespo EA, Aznar FJ. Patterns of trunk spine growth in two congeneric species of acantho-cephalan: investment in attachment may differ be-tween sexes and species. Parasitology 2012; 139 (7): 945-955.

Van Cleave HJ. Some host–parasite relation-ships of the Acanthocephala with special ref-erence to the organs of attachment. Exp Para-sitol. 1952; 1: 305–330.

Dezfuli BS, Sbrenna G. A morphological and ultrastructural study of Telosentis exiguus (Acanthocephala, Palaeacanthocephala). Boll Zool. 1990; 57: 225–232.

Hirschmann H. Scanning electron microscopy as a tool in nematode taxonomy. In: Stone A, Platt H and Khalil L (ed) Concepts in nematode systematics. Ac-ademic Press, New York, United States of America and London, United Kingdom. 1983; pp 95-111.

How to Cite
GUPTA N, K. GUPTA D, SINGHAL P. Description of Pallisentis (Brevitritospinus) punctati n. sp. (Acantho¬cephala: Quadrigyridae) from Channa punctatus in Ba¬reilly, Uttar Pradesh, India. Iran J Parasitol. 10(4):605-616.
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