Description of Pallisentis (Brevitritospinus) punctati n. sp. (Acantho¬cephala: Quadrigyridae) from Channa punctatus in Ba¬reilly, Uttar Pradesh, India
AbstractBackground: The genus Pallisentis is an endoparasitic acanthocephalan inhabiting the intestinal walls. Hooks and spines of the worm are significant taxonomical and adaptive tools.Methods: The parasites were fixed, dehydrated and examined under Olympus BX 53 Microscope with DIC attachment, digital camera and CELLSENS imaging system [Light microscopy (LM)] and ﬁxed in 2% glutaraldehyde in 0.1 M cacodylate buffer, dehydrated, rotary-coated with gold palladium in NeoCoater 100-240V and examined in Neo JCM-6000 [scanning electron microscopy (SEM)].Results: P. punctati n. sp. (prevalence 65.51%; mean intensity 3-6 par/host) is described. Females almost twice as large as males; proboscis hooks small; collar spine rows similar  and constant in both sexes but number of spines per row greater in females  than males ; trunk spine rows 28-39 (spines per row 14-18) in females and 20-26 (spines per row 10-12) in males. spine length of females almost twice as long as males, spines extend up to posterior testis in males and ¾ of total body length in females, Saefftigen’s pouch present, nuclei in cement gland 10-11, seminal vesicle, bursa and egg size small. SEM indicated lack of micro sculptures, and spines embedded on pre-trunk and trunk. Sex-based differences apparent (hook sizes, greater number of spines per row and longer spines in pre-trunk and trunk of females). Male trunk spine was narrower and of lateral spine with characteristic hooked appearance.Conclusion: A new species of Pallisentis based on LM and SEM is described, sexual diversity in hook and spine structure is reported.
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