Histopathological and Molecular Evaluation of the Experimentally Infected Goats by the Larval Forms of Taenia multiceps
,
Abstract
Background: Introduction of Taenia multiceps and T. gaigeri as two separate species have been recognized mainly on morphological grounds. This experimental study was conducted to test whether cerebral and non-cerebral forms of Coenurus cerebralis belong to one origin or they are originated from two different tape worms.
Methods: Two groups of dogs were infected with the cerebral and muscular sources of the coenuri cysts. About two months later the eggs were collected from the fecal samples to be used to experimentally infect other healthy goats. Histopathological and molecular evaluation was conducted in two groups of goats that were challenged with T. multiceps eggs obtained from the infected dogs by brain and muscular sources of coenuri cysts in School of Veterinary Medicine of Shiraz University, Shiraz, Iran in 2015. All aberrant sites of predilection of the metacestode in goats were muscles, heart, diaphragm and lungs. The brain and spinal cord were carefully dissected and examined but the cysts were not found in these locations. In addition, the molecular genetic markers of mitochondrial DNA (CO1 and ND1) were applied to resolve the questionable relationship between T. multiceps and T. gaigeri.
Results: The larval stages of T. multiceps in brain and in other aberrant sites, which showed similar morphological criteria, were monophyletic species.
Conclusion: Therefore, T. gaigeri must be considered taxonomically invalid.
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3. Akbari M, Moazeni M, Oryan A et al. Ex-perimental cerebral and non-cerebral coe-nurosis in goats: A comparative study on the morphological and molecular charac-teristics of the parasite. Vet Parasitol. 2015; 211(3-4):201-7.
4. Sharma DK, Chauhan PPS. Coenurosis status in Afro-Asian region: A review. Small Ruminant Res. 2006;64(3):197–202.
5. Amrabadi O, Oryan A, Moazeni M, Shari-fiyazdi H, Akbari M. Comparison of cere-bral and non-cerebral coenurosis by genet-ic markers of glycolytic enzyme (enolase) and mitochondrial sequences in sheep and goats. Vet Parasitol. 2015; 214(3-4):333-6.
6. Oryan A, Akbari M, Moazeni M, Amra-badi OR. Cerebral and non-cerebral coe-nurosis in small ruminants. Trop Biomed. 2014; 31(1):1-16.
7. Deressa A, Tilahun T, Tadesse A, Beyene M, Gebrewold G, Pal M. Assessment of Coenurus cerebralis and its economic impact in sheep brain harvested at Ethiopian Health and Nutrition Research Institue, Ethiopia. Int J Livest Res. 2012;2(2):217–26.
8. Nourani H, Kheirabadi KP. Cerebral coe-nurosis in a goat: pathological findings and literature review. Comp Clin Path. 2009;18(1):85–7.
9. Varcasia A, Jia WZ, Yan HB et al. Molecu-lar characterization of subcutaneous and muscular coenurosis of goats in United Arab Emirates. Vet Parasitol. 2012; 190(3-4):604-7.
10. Alim MA, Islam MK, Rahman MM, Khan MA, Mondan MM. Further observation on metacestodiosis in black Bengal goat in Bangladesh. Pak J Sci Ind Res. 2002;45(5):330–3.
11. Gosh RC, Dubey S, Mandal SC, Sharma N. Occurrence of Coenurus gaigeri cyst in a goat. Indian Vet J. 2005;82(1):90–1.
12. Islam S, Kalita D, Bhuyan D, Rahman T, Saleque A. Ocular coenurosis in goat. Vet Parasitol. 2006;20(1):53–5.
13. Moghaddar N. Occurrence of Coenurus gaigeri in goats in Shiraz, Iran. J Appl Anim Res. 2007;31(1):83–4.
14. Oryan A, Nazifi S, Sharifiyazdi H, Ahmad-nia S. Pathological, molecular, and bio-chemical characterization of Coenurus gaigeri in Iranian native goats. J Parasitol. 2010; 96(5):961-7.
15. Ibechukwu BI, Onwukeme KE. Intraocu-lar coenurosis: a case report. Br J Oph-thalmol. 1991; 75(7):430-1.
16. Ing MB, Schantz PM, Turner JA. Human coenurosis in North America: case reports and review. Clin Infect Dis. 1998;27(3):519–23.
17. Benifla M, Barrelly R, Shelef I et al. Huge hemispheric intraparenchymal cyst caused by Taenia multiceps in a child. Case report. J Neurosurg. 2007; 107(6 Suppl):511-4.
18. Collomb J, Machouart M, Biava MF et al. Contribution of NADH dehydrogenase subunit I and cytochrome C oxidase sub-unit I sequences toward identifying a case of human coenuriasis in France. J Parasi-tol. 2007; 93(4):934-7.
19. Oge H, Oge S, Gonenc B, Ozbakis G, Asti C. Coenurosis in the lumbar region of a goat: a case report. Vet Med. 2012;57(6):308–13.
20. Schuster RK, Sivakumar S, Wieckowsky T. Non-cerebral coenurosisin goats. Parasitol Res. 2010; 107(3):721-6.
21. Kheirandish R, Sami M, Azizi S, Mirzaei M. Prevalence, predilection sites and pathological findings of Taenia multiceps coenuri in slaughtered goats from south-east Iran. Onderstepoort J Vet Res. 2012; 79(1):E1-5.
22. Rausch RL. A consideration of infraspecif-ic categories in the genus Echinococcus Ru-dolphi, 1801 (Cestoda: Taeniidae). J Parasi-tol. 1967; 53(3):484-91.
23. Desouky EA, Badawy AI, Refaat RA. Sur-vey on coenurosis in sheep and goats in Egypt. Vet Ital. 2011; 47(3):333-40.
24. Rinaldi L, Coles GC, Maurelli MP, Musella V, Cringoli G. Calibration and diagnostic accuracy of simple flotation, McMaster and FLOTAC for parasite egg counts in sheep. Vet Parasitol. 2011; 177(3-4):345-52.
25. Zhang L, Hu M, Jones A, Allsopp BA et al. Characterization of Taenia madoquae and Taenia regis from carnivores in Kenya using genetic markers in nuclear and mitochon-drial DNA, and their relationships with other selected taeniids. Mol Cell Probes. 2007; 21(5-6):379-85.
26. Lavikainen A, Haukisalmi V, Lehtinen MJ et al. A phylogeny of members of the fam-ily Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology. 2008; 135(12):1457-67.
27. Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identi-fied by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54(2):165-73.
28. Bowles J, McManus DP. Genetic charac-terization of the Asian Taenia, a newly de-scribed taeniid cestode of humans. Am J Trop Med Hyg. 1994; 50(1):33-44.
29. NAGATY HF, EZZAT MA. On the identity of Multiceps multiceps (Leske, 1780), M. gaigeri Hall, 1916, and M. serialis (Gerva-lis, 1845), with a review of these and simi-lar forms in man and animals. Proc Hel-minthol Soc Wash. 1946; 13(2):33-44.
30. Loos-Frank B. An up-date of Verster’s 1969. ‘‘Taxonomic revision of the genus Taenia Linnaeus’’ (Cestoda) in table format. Syst Parasitol.. 2000;45(3):155–83.
31. Sharma DK, Singh N, Tiwari HA. Preva-lence and pathology of coenurosis in or-ganized goat farms. Vet Parasitol. 1998;12:30–2.
32. Verster A. A taxonomic revision of the genus Taenia Linnaeus, 1758s.str. Onder-stepoort J Vet Res. 1969;36(1):3–58.
33. McManus DP. Molecular genetic variation in Echinococcus and Taenia: An update. Southeast Asian J Trop Med Public Health. 1997; 28 Suppl 1:110-6..
34. Gasser RB, Zhu X, McManus DP. NADH dehydrogenase subunit 1and cyto-chrome c oxidase subunit I sequences compared for members of the genus Tae-nia (Cestoda). Int J Parasitol. 1999;29(12):1965–70.
35. Li WH, Jia WZ, Qu ZG, Xie ZZ et al. Mo-lecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1. Korean J Parasitol. 2013; 51(2):197-201.
36. Rostami S, Salavati R, Beech RN, Sharbat-khori M et al. Cytochrome c oxidase subu-nit 1 and 12S ribosomal RNA characteriza-tion of Coenurus cerebralis from sheep in Iran. Vet Parasitol. 2013; 197(1-2):141-51.
2. Oryan A, Moazeni M, Amrabadi OR, Ak-bari M, Sharifiyazdi H. Comparison of dis-tribution pattern, pathogenesis and molec-ularcharacteristics of larval stages of Taenia multiceps in sheep and goats. Small Rumi-nant Res. 2015;132:44–49.
3. Akbari M, Moazeni M, Oryan A et al. Ex-perimental cerebral and non-cerebral coe-nurosis in goats: A comparative study on the morphological and molecular charac-teristics of the parasite. Vet Parasitol. 2015; 211(3-4):201-7.
4. Sharma DK, Chauhan PPS. Coenurosis status in Afro-Asian region: A review. Small Ruminant Res. 2006;64(3):197–202.
5. Amrabadi O, Oryan A, Moazeni M, Shari-fiyazdi H, Akbari M. Comparison of cere-bral and non-cerebral coenurosis by genet-ic markers of glycolytic enzyme (enolase) and mitochondrial sequences in sheep and goats. Vet Parasitol. 2015; 214(3-4):333-6.
6. Oryan A, Akbari M, Moazeni M, Amra-badi OR. Cerebral and non-cerebral coe-nurosis in small ruminants. Trop Biomed. 2014; 31(1):1-16.
7. Deressa A, Tilahun T, Tadesse A, Beyene M, Gebrewold G, Pal M. Assessment of Coenurus cerebralis and its economic impact in sheep brain harvested at Ethiopian Health and Nutrition Research Institue, Ethiopia. Int J Livest Res. 2012;2(2):217–26.
8. Nourani H, Kheirabadi KP. Cerebral coe-nurosis in a goat: pathological findings and literature review. Comp Clin Path. 2009;18(1):85–7.
9. Varcasia A, Jia WZ, Yan HB et al. Molecu-lar characterization of subcutaneous and muscular coenurosis of goats in United Arab Emirates. Vet Parasitol. 2012; 190(3-4):604-7.
10. Alim MA, Islam MK, Rahman MM, Khan MA, Mondan MM. Further observation on metacestodiosis in black Bengal goat in Bangladesh. Pak J Sci Ind Res. 2002;45(5):330–3.
11. Gosh RC, Dubey S, Mandal SC, Sharma N. Occurrence of Coenurus gaigeri cyst in a goat. Indian Vet J. 2005;82(1):90–1.
12. Islam S, Kalita D, Bhuyan D, Rahman T, Saleque A. Ocular coenurosis in goat. Vet Parasitol. 2006;20(1):53–5.
13. Moghaddar N. Occurrence of Coenurus gaigeri in goats in Shiraz, Iran. J Appl Anim Res. 2007;31(1):83–4.
14. Oryan A, Nazifi S, Sharifiyazdi H, Ahmad-nia S. Pathological, molecular, and bio-chemical characterization of Coenurus gaigeri in Iranian native goats. J Parasitol. 2010; 96(5):961-7.
15. Ibechukwu BI, Onwukeme KE. Intraocu-lar coenurosis: a case report. Br J Oph-thalmol. 1991; 75(7):430-1.
16. Ing MB, Schantz PM, Turner JA. Human coenurosis in North America: case reports and review. Clin Infect Dis. 1998;27(3):519–23.
17. Benifla M, Barrelly R, Shelef I et al. Huge hemispheric intraparenchymal cyst caused by Taenia multiceps in a child. Case report. J Neurosurg. 2007; 107(6 Suppl):511-4.
18. Collomb J, Machouart M, Biava MF et al. Contribution of NADH dehydrogenase subunit I and cytochrome C oxidase sub-unit I sequences toward identifying a case of human coenuriasis in France. J Parasi-tol. 2007; 93(4):934-7.
19. Oge H, Oge S, Gonenc B, Ozbakis G, Asti C. Coenurosis in the lumbar region of a goat: a case report. Vet Med. 2012;57(6):308–13.
20. Schuster RK, Sivakumar S, Wieckowsky T. Non-cerebral coenurosisin goats. Parasitol Res. 2010; 107(3):721-6.
21. Kheirandish R, Sami M, Azizi S, Mirzaei M. Prevalence, predilection sites and pathological findings of Taenia multiceps coenuri in slaughtered goats from south-east Iran. Onderstepoort J Vet Res. 2012; 79(1):E1-5.
22. Rausch RL. A consideration of infraspecif-ic categories in the genus Echinococcus Ru-dolphi, 1801 (Cestoda: Taeniidae). J Parasi-tol. 1967; 53(3):484-91.
23. Desouky EA, Badawy AI, Refaat RA. Sur-vey on coenurosis in sheep and goats in Egypt. Vet Ital. 2011; 47(3):333-40.
24. Rinaldi L, Coles GC, Maurelli MP, Musella V, Cringoli G. Calibration and diagnostic accuracy of simple flotation, McMaster and FLOTAC for parasite egg counts in sheep. Vet Parasitol. 2011; 177(3-4):345-52.
25. Zhang L, Hu M, Jones A, Allsopp BA et al. Characterization of Taenia madoquae and Taenia regis from carnivores in Kenya using genetic markers in nuclear and mitochon-drial DNA, and their relationships with other selected taeniids. Mol Cell Probes. 2007; 21(5-6):379-85.
26. Lavikainen A, Haukisalmi V, Lehtinen MJ et al. A phylogeny of members of the fam-ily Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology. 2008; 135(12):1457-67.
27. Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identi-fied by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54(2):165-73.
28. Bowles J, McManus DP. Genetic charac-terization of the Asian Taenia, a newly de-scribed taeniid cestode of humans. Am J Trop Med Hyg. 1994; 50(1):33-44.
29. NAGATY HF, EZZAT MA. On the identity of Multiceps multiceps (Leske, 1780), M. gaigeri Hall, 1916, and M. serialis (Gerva-lis, 1845), with a review of these and simi-lar forms in man and animals. Proc Hel-minthol Soc Wash. 1946; 13(2):33-44.
30. Loos-Frank B. An up-date of Verster’s 1969. ‘‘Taxonomic revision of the genus Taenia Linnaeus’’ (Cestoda) in table format. Syst Parasitol.. 2000;45(3):155–83.
31. Sharma DK, Singh N, Tiwari HA. Preva-lence and pathology of coenurosis in or-ganized goat farms. Vet Parasitol. 1998;12:30–2.
32. Verster A. A taxonomic revision of the genus Taenia Linnaeus, 1758s.str. Onder-stepoort J Vet Res. 1969;36(1):3–58.
33. McManus DP. Molecular genetic variation in Echinococcus and Taenia: An update. Southeast Asian J Trop Med Public Health. 1997; 28 Suppl 1:110-6..
34. Gasser RB, Zhu X, McManus DP. NADH dehydrogenase subunit 1and cyto-chrome c oxidase subunit I sequences compared for members of the genus Tae-nia (Cestoda). Int J Parasitol. 1999;29(12):1965–70.
35. Li WH, Jia WZ, Qu ZG, Xie ZZ et al. Mo-lecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1. Korean J Parasitol. 2013; 51(2):197-201.
36. Rostami S, Salavati R, Beech RN, Sharbat-khori M et al. Cytochrome c oxidase subu-nit 1 and 12S ribosomal RNA characteriza-tion of Coenurus cerebralis from sheep in Iran. Vet Parasitol. 2013; 197(1-2):141-51.
| Files | ||
| Issue | Vol 14 No 1 (2019) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijpa.v14i1.722 | |
| Keywords | ||
| Coenurosis Coenurus cerebralis DNA (CO1 and ND1) Taenia gaigeri Taenia multiceps | ||
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How to Cite
1.
AMRABADI O, ORYAN A, MOAZENI M, SHARIFIYAZDI H, AKBARI M. Histopathological and Molecular Evaluation of the Experimentally Infected Goats by the Larval Forms of Taenia multiceps. Iran J Parasitol. 2019;14(1):95-105.
