Influence of Toxoplasma gondii Infection on Male Fertility: A Pilot Study on Immunocompetent Human Volunteers

  • Horaţiu Alexandru COLOSI Dept. of Medical Informatics and Biostatistics, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Babak JALALI-ZADEH Dept. of Urology, Municipal Hospital, Dej, Romania
  • Ioana Alina COLOSI Dept. of Microbiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Laura Mihaela SIMON Dept. of Microbiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
  • Carmen Anca COSTACHE Dept. of Microbiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
Keywords: latent toxoplasmosis, TOX-IgG, spermiogram, FSH, testosterone

Abstract

Background: This study was conducted to investigate the influence of Toxoplasma gondii infection on spermatic and hormonal parameters in a pilot sample of immunocompe­tent human male subjects.Methods: This cross sectional, observational pilot study on 60 immunocompetent hu­man male subjects aged between 18 and 60 yr old was conducted between 2012 -2013. Blind evaluation of serological markers of past T. gondii infection (TOX-IgG, TOX-IgM) was performed, along with individual spermiograms and determinations of folli­cle-stimulating hormone (FSH) and testosterone serum levels.Results: The overall prevalence of past T. gondii infection in the investigated immunocom­petent male subjects was 25%. No statistically significant influence of T. gondii infection on sperm characteristics (ejaculate quantity, sperm count, motility, morphol­ogy) and serum levels of FSH or testosterone were found. Among possible predictors of a modified spermiogram studied by multiple logistic regression along with the T. gondii infection (age, smoking, alcohol consumption, fertility influencing malfor­mations, infections, trauma or medication), only the presence of varicocele in the medi­cal history of the studied subjects was found to significantly participate in the predic­tion of a modified spermiogram (P=0.0154). A necessary sample size of 994 subjects was computed in order to achieve a test power of 0.8 (80%) to discriminate an effect size of 8.89% estimated by our pilot study.Conclusions: Although our investigation did not demonstrate an influence of latent T. gondii infection on spermatic and hormonal parameters of immunocompetent male hu­mans, the absence of such an influence cannot be affirmed, due to the limited sample size of our pilot study. 

References

Povey AC, Stocks SJ. Epidemiology and trends in male subfertility. Hum Fertil (Camb). 2010;13:182-8.

Krausz C. Male infertility: pathogenesis and clinical diagnosis. Best Pract Res Clin Endocrinol Metab. 2011;25:271-85.

Ajzenberg D. Unresolved questions about the most successful known parasite. Expert Rev Anti Infect Ther. 2011;9:169-71.

Carme B, Bissuel F, Ajzenberg D, Bouyne R, Aznar C, Demar M, et al. Severe acquired toxoplasmosis in immunocompetent adult patients in French Guiana. J Clin Microbiol. 2002;40:4037-44.

Berrebi A, Bardou M, Bessieres MH, et al. Outcome for children infected with congenital toxoplasmosis in the first trimester and with normal ultrasound findings: a study of 36 cases. Eur J Obstet Gynecol Reprod Biol. 2007;135:53-7.

Costache CA, Colosi HA, Blaga L, et al. First isolation and genetic characterization of a Toxoplasma gondii strain from a symptomatic human case of congenital toxoplasmosis in Romania. Parasite. 2013;20:11.

Villena I, Ancelle T, Delmas C, Garcia P, Brezin AP, Thulliez P, et al. Congenital toxoplasmosis in France in 2007: first results from a national surveillance system. Euro Surveill. 201015:19600.

Nistal M, Santana A, Paniaqua R, Palacios J. Testicular toxoplasmosis in two men with the acquired immunodeficiency syndrome (AIDS). Arch Pathol Lab Med. 1986;110:744-6.

Haskell L, Fusco MJ, Ares L, Sublay B. Disseminated toxoplasmosis presenting as symptomatic orchitis and nephrotic syndrome. Am J Med Sci. 1989;298:185-90.

Martínez-García F, Regadera J, Mayer R, Sanchez S, Nistal M. Protozoan infections in the male genital tract. J Urol. 1996;156:340-9.

Terpsidis KI, Papazahariadou MG, Taitzoglou IA, Papaioannou NG, Georgiadis MP, Theodoridis IT. Toxoplasma gondii: reproductive parameters in experimentally infected male rats. Exp Parasitol. 2009;121:238-41.

Lim A, Kumar V, Hari Dass SA, Vyas A. Toxoplasma gondii infection enhances testicular steroidogenesis in rats. Molecular Ecology. 2013;22:102–10.

Dvorakova-Hortova K, Sidlova A, Ded L, Hladovcova D, Vieweg M, Weidner W, et al. Toxoplasma gondii decreases the reproductive fitness in mice. PLoS One. 2014; 9:e96770.

Qi R, Su XP, Gao XL, Liang XL. Toxoplasma infection in males with sterility in Shenyang, China. Zhonghua Nan Ke Xue. 2005;11:503-4.

Hertzog MA. Considerations in determining sample size for pilot studies. Res Nurs Health. 2008;31:180-91.

Costache CA, Tigan S, Colosi I, Coroiu Z. Toxoplasmic infection in pregnant women from Cluj County and neighbouring areas. Applied Medical Informatics 2008;23:31-6.

Coroiu Z, Radu R, Molnar A, Bele J. Seroprevalence of anti Toxoplasma gondii antibodies in the healthy population from north-western and central Romania. Scientia Parasitologica. 2009;10:37-42.

Dubey JP, Hotea I, Olariu TR, Jones JL, Dărăbuş G. Epidemiological review of toxoplasmosis in humans and animals in Romania. Parasitology 2014;141:311-25.

Abdoli A, Dalimi A, Movahedin M. Impaired reproductive function of male rats infected with Toxoplasma gondii. Andrologia. 2012;44 Suppl 1:679-87.

Flegr J, Hrusková M, Hodný Z, Novotná M, Hanusová J. Body height, body mass index, waist-hip ratio, fluctuating asymmetry and second to fourth digit ratio in subjects with latent toxoplasmosis. Parasitology. 2005; 130:621-8.

Galván-Ramírez ML, Gutiérrez-Maldonado AF, Verduzco-Grijalva F, Jiménez JM. The role of hormones on Toxoplasma gondii infection: a systematic review. Front Microbiol. 2014;5:503.

Published
2015-10-17
How to Cite
1.
COLOSI HA, JALALI-ZADEH B, COLOSI IA, SIMON LM, COSTACHE CA. Influence of Toxoplasma gondii Infection on Male Fertility: A Pilot Study on Immunocompetent Human Volunteers. IJPA. 10(3):402-9.
Section
Original Article(s)