Iranian Journal of Parasitology 2013. 8(1):167-170.

Activity of Superoxide Dismutase (SOD) Enzyme in the Excretory-Secretory Products of Fasciola hepatica and F. gigantica Parasites.
A Farahnak, A Golestani, Mr Eshraghian

Abstract


 

Background: The aim of this study was to compare superoxide dismutase (SOD) activity in Fasciola hepatica and Fasciola gigantica parasites.

Materials: F. gigantica and F. hepatica helminths were collected from abattoir and cultured in buffer media for 4 h at 37 °C. Excretory-Secretory (ES) products were collected, centrifuged and stored at - 20◦C. E-S protein concentration was measured by Bradford method and SOD activity was detected using RANSOD kit (Randox Lab. Crumlin, UK). Statistical t-test was conducted for analysis of re-sults.

Results: Protein concentration for F. hepatica and F. gigantica were obtained 7.293 ug/ml and 19.65 ug/ml respectively and SOD activity as 0.721 U/ml and 1.189 U/ml , in that order. ES protein con-centration of two species was significantly different (P<0.05), however the difference of SOD activi-ty of two species was not significant.

Conclusion: Two species of Fasciola have comparable SOD biochemical defense enzyme and can help us explain the parasite survival in host tissue.


Keywords


Excretory-Secretory; Fasciola; Helminth; Superoxide Dismutase

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References


Mas-Coma MS, Esteban JG, Bargues MD. Epidemiology of human fascioliasis. Bull World Health Organ. 1999; 77(4):340-6.

http://www.greatvistachemicals.com/biochemicals/superoxide_dismutase.html

Piacenza L, Radi R, Goñi F, Carmona C. Cu Zn superoxide dismutase activities from Fas-ciola hepatica. Parasitology.1998; 117(6):555-62.

Maizels RM, Blaxter ML, Robertson BD, Selkirk ME (1991). Parasite antigen and para-site genes: A laboratory manual for molecular parasitology.1st ed. Cambridge University Press, UK, pp.:93-5.

Simon R. Protein purification techniques; A practical approach. Second edition, USA: Oxford University Press; 2001.

Ransod superoxide dismutase manual. http://www.randox.com. 7. Russell M. Morphew, Hazel A. Wright, E. James LaCourse, Debra J. Woods and Peter M. Brophy. Comparative Proteomics of Excretory-Secretory Proteins Released by the Liver Fluke Fasciola hepatica in Sheep Host Bile and during in Vitro Culture ex host. Mo-lecular & Cellular Proteomics.2007; 6(6):963.

James R. Jefferies, Alison M. Campbell, Arjan J. van Rossum, John Barrett, Peter M. Brophy. Proteomic analysis of Fasciola hepatica products. Proteomics. 2001; 1(9):1128–32.

Piacenza L, Radi R, Goñi F, Carmona C. CuZn superoxide dismutase activities from Fasciola hepatica. Parasitology. 1998; 117(6):555.

A. El-Ghaysha, R. J. Turnerb, P. M. Brophy and J. Barrett. Effect of Fasciola gigantica so-matic extracts and excretory/secretory prod-ucts on superoxide production by activated neutrophils. Vet Parasitol.1999; 84(1):91-100.


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