Identification of Tropomyosin and Its Immunological Properties from Larvae of Cattle Tick, Boophilus annulatus.

  • S Nabian Department of Parasitology and Research Center of Ticks and Tick Borne Disease, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • M Taheri Rastegar Reference Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • R Mazaheri Nezhad Fard Rastegar Reference Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • M Aramoon Arian Hospital, Gonbad Qaboos, Iran
Keywords: Boophilus annulatus, Tropomyosin, Vaccine


 Background: Boophilus annulatus is an obligate blood feeder tick that can cause great losses in animals due to anemia and its ability to injure its host skin directly. The aim of this study was identification of cattle humoral immune response to some tick proteins during experimental infestation. Methods: Immune sera against tick were collected from experimentally infested cattle with ticks. One and two-dimensional electrophoresis and Western blotting methods were used for the detection of immunogenic proteins in larval tick extract and eight of these proteins were identified by MALDI-TOF and MALDI-TOF-TOF mass spectrometry. Results: In non-reducing one-dimensional SDS- PAGE, some bounds between 12 to more than 250-kDa appeared. In two-dimensional SDS-PAGE, numerous spot appeared and the identified immunogenic proteins by parallel immunoblotting weighted between 14 and 97 kDa. Amino acid sequences of protein spot with 37-kDa molecular weight had identity to tropomyosin based on Mas-cot search in NCBI. Conclusion: Anti tropomyosin antibodies can be induced in experimentally infested hosts with ticks and it seems that tropomyosin can be useful for the development of anti tick vaccines.


Estrada-Pena A, Bouattour A, Camicas JL, Walker AR. Ticks of domestic animals in the Mediterranean region. A guide to iden-tification of species. Zaragoza: University of Zaragoza, 2004.

Gindin G, Samish M, Zangi G, Mishout-chenko A, Glazer L. The susceptibility of different species and stages of ticks to entomopathogenic fungi. Exp Appl Acarol. 2002; 28: 283–288.

Willadsen P, Smith D, Cobon G, Mckenna RV. Comparative vaccination of cattle against Boophilus micropolus with recombi-nant antigen Bm86 alone or in combina-tion with recombinant Bm91. Parasite Immunol. 1996; 18: 241–246.

Imamura S, da Siva Vaz I, Sugino M, Oha-shi K, Onuma M. A serine protease inhibi-tor (serpin) from Haemaphysalis longicornis as an anti-tick vaccine. Vaccine. 2005; 23: 1301–1311.

Nisbet AJ, Huntley JF, Mackellar A, Sparks N, McDevitt R. A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer). Parasite Immunol. 2006; 28: 401–5.

Gunning PW, Schevzov G, Kee AJ, Har-deman EC. Tropomyosin isoforms: di-vining rods for actin cytoskeleton function. Trends Cell Biol. 2005; 15: 333–341.

Brown SJ, Shapiro SZ, Askenase PW. Cha-racterization of tick antigens inducing host immune resistance. J Immunol. 1984; 132: 3319–3325.

Lamelli UK. Cleavage of structural proteins during the assembly of head bacteriophage T4. Nature. 1970; 227: 680–685.

Yatsuda AP, Krijgsveld J, Cornelissen AW, Heck AJ, de Vries E. Comprehensive anal-ysis of the secreted proteins of the parasite Haemonchus contortus reveals extensive se-quence variation and differential immune recognition. J Biol Chem. 2003; 278: 16941–51.

Wang H, Nuttal PA. Comparison of the proteins in salivary glands, saliva and haemolymph of Ripicephalus appendiculatus female ticks during feeding. Parasitology. 1994; 109: 517–523.

Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, weiss W. The current state of two- dimensional ele-cterophoresis with immobilized pH gra-dients. Electrophoresis, 2000; 21: 1037–1053.

Hooper SL, Thuma JB. Invertebrate mus-cles: Muscle specific gene and proteins. Physiol Rev. 2005; 85: 1001–1060.

Perry SV. Vertebrate tropomyosin: distri-bution, properties and function. J Muscle Res Cell Motil. 2001; 22: 5–49.

Zhao HP, Yin H, Li CY, Gao JL, Li YQ, Guan GQ, Liu ZJ, Liu JL, Yang FH, Xing XM, Luo JX. Construction of larval cDNA expression library and immunological iden-tification of positive clones in tick Haema-physalis qinghaiensis. Agr Sci China. 2010; 9: 896–902.

Aki T, Kodama T, Fujikawa A Miura K, Shigeta S, Wada T, Jyo T, Murooka Y, Oka S, Ono K. Immunochemical characteriza-tion of recombinant and native tropomyo-sins as a new allergen from the house dust mite, Dermatophagoides farina. J Allergy Clin Immunol. 1995; 96: 74–83.

Huntley JF, Machell J, Nisbet AJ, Van den Broek A, Chua KY, Cheong N, Hales BJ, Thomas WR. Identification of tropomyosin, paramyosin and apolipophorin/vitellogenin as three major allergens of the sheep scab mite, Psoroptes ovis. Parasite Immunol. 2005; 26: 335–342.

Hartmann S, Adam R, Marti T, Kirsten C, Seidinger S, Lucius R. A 41-kDa antigen of the rodent filaria Acanthocheilonema viteae with homologies to tropomyosin induces host-protective immune responses. Parasitol Res. 1997; 83: 390–3.

Rosalind EJ, Taylor MJ, Gilvary NJ, Bianco AE. Tropomyosin implicated in host pro-tective responses to microfilariae in onc-hocerciasis. Proc Natl Acad Sci USA. 1998; 95: 7550–7555.

Folkard SG, Taylor MJ, Butcher GA, Bi-anco AE. Protective responses against skin-dwelling microfilariae of Onchocerca lienalis in severe combined immunodefi-cient mice. Inf Immunol. 1997; 65: 2846–2851.

Untalan PM, Guerrero FD, Haines LR, Pearson TW. Proteome analysis of abun-dantly expressed proteins from unfed lar-vae of the cattle tick, Boophilus micropolus. In-

How to Cite
Nabian S, Taheri M, Fard RMN, Aramoon M. Identification of Tropomyosin and Its Immunological Properties from Larvae of Cattle Tick, Boophilus annulatus. Iran J Parasitol. 8(2):242-8.