Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp. in Sheep and Goat Blood Samples from Pakistan.

  • F Iqbal Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University Multan 60800, Pakistan.
  • Rm Khattak Department of Zoology, Kohat University of Science and Technology, Kohat, Pakistan
  • S Ozubek Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey
  • Mnk Khattak Department of Zoology, University of Hazara, Mansehra, Pakistan
  • A Rasul Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130021, China
  • M Aktas Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey
Keywords: 18S rRNA gene, Goat, RLB, Sheep, Theileria, Babesia


 Background: The present study was designed to detect the presence of tick-borne parasites (Theileria and Babesia spp.) in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan. Methods: Reverse line blot (RLB) assay was applied for the parasitic detection by the amplification of hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene. A membrane with covalently linked generic and species specific oligonucleotide probes was used for the hybridization of amplified PCR products. Results: Parasites were detected in 16% of the ruminant blood samples under study. Two Theileria species, T. lestoquardi and T. ovis, were identified in samples. 25, of the total 32, infected animals were from Khyber Pukhtoon Khwa. Conclusion: Sheep were more prone to tick borne haemoprotozans as 81% infected samples were sheep as compared to 19% goats (P > 0.001). Risk factor analysis revealed that male (P = 0.03), ani-mals infested by ticks (P = 0.03) and herd composed of sheep only (P = 0.001) were more infected by blood parasites.


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How to Cite
Iqbal F, Khattak R, Ozubek S, Khattak M, Rasul A, Aktas M. Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp. in Sheep and Goat Blood Samples from Pakistan. Iran J Parasitol. 8(2):289-295.