Validation of PCR Assay for Identification of Sarcoptes scabiei var. hominis.

  • Shumaila Naz Dept. of Zoology, Faculty of Sciences, University of Pir Mehr Ali Shah- Arid Agriculture, Rawalpindi, Pakistan.
  • Dilwar Abbas Rizvi Institute of Dermatology, Military Hospital of Rawalpindi, Pakistan.
  • Amara Javaid Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan.
  • Muhammad Ismail Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan.
  • Farhana Riaz Chaudhry Dept. of Zoology, Faculty of Sciences, University of Pir Mehr Ali Shah- Arid Agriculture, Rawalpindi, Pakistan.
Keywords: DNA extraction, Itch mite DNA, PCR technique, Sarcoptes scabiei

Abstract

 Background: Infestation of the skin by the “itch mite” Sarcoptes scabiei var. hominis results in a contagious skin infection in humans called “sca-bies”. By resolving morphology issues, the present study was designed to be acquainted with itch mite by molecular markers. Methods: The mite samples were collected from scabies patients by visiting government hospitals of twin City, Pakistan. For successful mo-lecular detection approach, preparation of Sarcoptes mite DNA by com-mercial DNA extraction kit method. Furthermore, two primers i.e. Sarms 15 F/R and 16S D1/D2 were used to amplify target sequence by using PCR. The amplified products were then separated by agarose gel, electrophoresis and analyzed after staining and visualizing in UV transilluminator. Results: Analysis of PCR product showed one specific band of 178 bp with primer Sarms 15 F/R, while, with primer 16S D1/D2 bands of 460 bp and 600 bp were observed on 2% agarose gel. The appearance of different band of 600 bp revealed that it might be due to heteroplasmy state present in the Pakistani Sarcoptes mites population. Conclusion: Current study adds validity to the claim that PCR is more accurate, specific and sensitive in the detection of the ectoparasites even in smallest amount.

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How to Cite
1.
Naz S, Rizvi DA, Javaid A, Ismail M, Chaudhry FR. Validation of PCR Assay for Identification of Sarcoptes scabiei var. hominis. IJPA. 8(3):437-40.
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