Iranian Journal of Parasitology 2013. 8(4):545-551.

Genetic Variability of Antigen B2 of Human, Sheep, Goats, Camel and Cattle Isolates of Echinococcus granulosus in Iran.
Bahador Sarkari Shahriari, Elahe Biranvand, Seyed Mahmoud Sadjjadi, Hamidreza Rahimi



Background: Antigen B (AgB) is frequently used for immuno-diagnosis of hu-man cystic echinococcosis (CE). Echinococcus granulosus AgBs show a high degree of genetic variability in different hosts or in different CE endemic areas. The present study aimed to evaluate the genetic polymorphisms of encoding antigen B2 gene (AgB2) among different Iranian isolates of E. granulosus.

Methods: A total of 50 CE isolates were collected from human, sheep, cattle, goat and camels, 10 isolates from each intermediate host of E. granulosus. Total genomic DNA from either protoscolices or germinal layer was extracted from each cyst and PCR-RFLP followed by DNA sequencing was used to evaluate sequence variation and polymorphism of AgB2 in the isolates.

Results: After the PCR amplification, using AgB2 primers, an almost 400 bp band was amplified in all of the isolates. The PCR products were digested with Alu1 re-striction endonuclease. After restriction enzyme digestion with Alu1‚ sheep and human isolates gave a similar pattern of RFLP with the gene size of approximately 140 and 240bp and camel and goat isolates gave a similar pattern, but different from sheep and human, with the gene size of approximately 150 and 250bp. Se-quence analysis showed the most genetic similarity of AgB2 between human and sheep isolates.

Conclusion: Findings of this study revealed the differences in the sequences of AgB2 within and between the Iranian isolates of E. granulosus. These differences may affect the performance of any diagnostic test which uses AgB.


Antigen B2; Echinococcus granulosus; Genetic variability; Iran

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Sadjjadi SM. Present situation of echinococ-cosis in the Middle East and Arabic North Af-rica. Parasitol Int. 2006; 55:S197-202.

Rokni MB. Echinococcosis /hydatidosis in Iran. Iranian J Parasitol. 2009; 4(2):1-16.

Sarkari B, Sadjjadi SM, Beheshtian MM, Aghaee M, Sedaghat F. Human cystic echino-coccosis in Yasuj district in Southwest of Iran: an epidemiological study of seroprevalence and surgical cases over a ten-year period. Zoonoses Public Health. 2010; 57(2):146-50.

Mamuti W, Sako Y, Nakao M, Xiao N, Nakaya K, Ishikawa Y, Yamasaki H, Lightowlers MW, Ito A. Recent advances in characterization of Echinococcus antigen B. Parasitol Int. 2006; 55:S57-62.

Monteiro KM, Zaha A, Ferreira HB. Recombi-nant subunits as tools for the structural and functional characterization of Echinococcus granu-losus antigen B. Exp Parasitol. 2008; 119(4):490-8.

Mohammadzadeh T, Sako Y, Sadjjadi SM, Sarkari B, Ito A. Comparison of the usefulness of hydatid cyst fluid, native antigen B and re-combinant antigen B8/1for serological diagno-sis of cystic echinococcosis. Trans R Soc Trop Med Hyg.2012; 106(6):371-5.

Rahimi H, Sadjjadi S, Sarkari B. Performance of antigen B isolated from different hosts and cyst locations in diagnosis of cystic echinococ-cosis. Iranian J Parasitol. 2011; 6(1):12-9.

Sadjjadi SM, Abidi H, Sarkari B, Izadpanah A, Kazemian S. Evaluation of enzyme linked im-munosorbent assay, utilizing native antigen B for serodiagnosis of human hydatidosis. Iran J Immunol. 2007; 4(3):167-72.

Carmena D, Benito A, Eraso E. Antigens for the immunodiagnosis of Echinococcus granulosus infection: An update. Acta Trop. 2006; 98(1):74-86.

Sarkari B, Sadjjadi SM, Abidi H, Izadpanah A, Kazemian S, Rafati A. Application of western blotting using native antigen B for serodiagno-sis of human cystic echinococcosis. Iranian J Parasitol. 2007; 2(3):7-12.

Monteiro KM, Cardoso MB, Follmer C, da Silveira NP, Vargas DM, Kitajima EW, Zaha A, Ferreira HB. Echinococcus granulosus antigen B structure: subunit composition and oligomeric states. PLoS Negl Trop Dis. 2012; 6(3):e1551.

Zhang W, Li J, Jones MK, Zhang Z, Zhao L, Blair D, McManus DP. The Echinococcus granu-losus antigen B gene family comprises at least 10 unique genes in five subclasses which are dif-ferentially expressed. PLoS Negl Trop Dis. 2010; 4(8):e784.

Fernandez V, Ferreira HB, Fernandez C, Zaha A, Nieto A. Molecular characterization of a novel 8-kDa subunit of Echinococcus granulosus antigen B. Mol Biochem Parasitol. 1996; 77: 247-250.

Tawfeek GM, Elwakil HS, Awad NS, El-Ho-seiny L, Thabet HS, Sarhan RM, Darweesh SK, Anwar WA. Genetic variability of antigen B among Echinococcus granulosus Egyptian isolates. Korean J Parasitol. 2009; 47(3):259-64.

Arend AC, Zaha A, Ayala FJ, Haag KL. The Echinococcus granulosus antigen B shows a high degree of genetic variability. Exp Parasitol. 2004; 108(1-2):76-80.

Kamenetzky L, Muzulin PM, Gutierrez AM, Angel SO, Zaha A, Guarnera EA, Rosenzvit MC. High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus. Parasitology. 2005; 131(6):805-15.


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