In Vitro Inhibitory Effect of Berberis vulgaris (Berberidaceae) and Its Main Component, Berberine against Different Leishmania Species.

  • Hossein Mahmoudvand Dept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran AND Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
  • Fariba Sharififar Dept. of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
  • Iraj Sharifi Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
  • Behrouz Ezatpour Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
  • Majid Fasihi Harandi Dept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
  • Mahsa Sadat Makki Dept. of Medical Parasitology, Tehran University of Medical Sciences, Tehran, Iran.
  • Naser Zia-Ali Dept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
  • Sareh Jahanbakhsh Dept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran AND Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Keywords: Berberine, Berberis vulgaris, In vitro, Leishmania infantum, Leishmania tropica

Abstract

 Background: Leishmaniasis has been identified as a major public health problem in tropical and sub-tropical countries. The present study was aimed to investigate antileishmanial effects of various extracts of Berberis vulgaris also its active com-poenent, berberine against Leishmania tropica and L. infantum species on in vitro ex-periments. Methods: In this study in vitro antileishmanial activity of various extracts of B. vul-garis also its active compoenent, berberine against promastigote and amastigote stages of L. tropica and L. infantum was evaluated, using MTT assay and in a macro-phage model, respectively. Furthermore, infectivity rate and cytotoxicity effects of B. vulgaris and berberine in murine macrophage cells were investigated. Results: The findings of optical density (OD) and IC50 indicated that B. vulgaris particulary berberine significantly (P<0.05) inhibited the growth rate of pro-mastigote stage of L.tropica and L.infantum in comparison to meglumine antimoniate (MA). In addition, B. vulgaris and berberine significantly (P<0.05) decreased the mean number of amastigotes in each macrophage as compared with positive con-trol. In the evaluation of cytotoxicity effects, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages. Results also showed that when parasites were pre-incubated with B. vulgaris their ability to infect murine macrophages was significantly decreased. Conclusion: B.vulgaris particularly berberine exhibited potent in vitro leishmanicid-al effects against L. tropica and L.infantum. Further works are required to evaluate the antileishmanial effects of B.vulgaris on Leishmania species using clinical settings.

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How to Cite
1.
Mahmoudvand H, Sharififar F, Sharifi I, Ezatpour B, Fasihi Harandi M, Makki MS, Zia-Ali N, Jahanbakhsh S. In Vitro Inhibitory Effect of Berberis vulgaris (Berberidaceae) and Its Main Component, Berberine against Different Leishmania Species. IJPA. 9(1):28-6.
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