Acanthamoeba species in Swimming Pools of Cairo, Egypt.

  • Ahmad Al-Herrawy Parasitology Laboratory, Water Pollution Research Department, NRC, 12622 Dokki, Giza, Egypt.
  • Mahmoud Bahgat Therapeutic Chemistry Department, NRC, 12622 Dokki, Giza, Egypt.
  • Abd-Elhafez Mohammed Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
  • Ameen Ashour Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
  • Wafaa Hikal Parasitology Laboratory, Water Pollution Research Department, NRC, 12622 Dokki, Giza, Egypt.
Keywords: Acanthamoeba, Cyst morphology, Egypt, PCR, Swimming pools


Background: The free-living amoebae Acanthamoeba spp. have been recognized as etiologic agents of amoebic encephalitis, keratitis, otitis, lung lesions and other skin infections mainly in immuno-compromised individuals. The purpose of this study is to detect the presence of Acanthamoeba in swimming pools in Egypt using a polymerase chain reaction (PCR) method.Methods: Water samples were collected from 10 different swimming pools in Cai-ro, Egypt. Samples were cultured on non-nutrient agar for the detection of Acan-thamoeba isolates that were confirmed by PCR amplification using genus specific primers. The molecularly confirmed Acanthamoeba isolates were morphologically identified to the species level.Results: Members of genus Acanthamoeba were detected in 49.2% of the examined swimming-pool water samples. Morphologically, six Acanthamoeba species were iso-lated from the examined swimming pool water namely A. polyphaga, A.castellanii, A. rhysodes, A. mauritaniensis, A. royreba and A. triangularis. All the identified species of Acanthamoeba were molecularly confirmed to be related to the genus Acanthamoeba. Conclusion: The isolated species of Acanthamoeba could provoke variable degrees of infections to the swimmers. The culture method is cheaper and easier than PCR techniques that are faster for the detection of free-living amoebae.


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How to Cite
Al-Herrawy A, Bahgat M, Mohammed A-E, Ashour A, Hikal W. Acanthamoeba species in Swimming Pools of Cairo, Egypt. IJPA. 9(2):194-01.