First Molecular Identification of Sarcocystis ovicanis (Protozoa, Apicomplexa) in the Brain of Sheep in Iran.
AbstractBackground: The objective of the present study was to survey the presence of Sarcocystis in sheep's brain in North Khorasan Province. Methods: In general, 80 samples of sheep's brain were collected from slaugh-tered sheep in slaughterhouses of North Khorasan Province. Tissue digestion method was used for observing bradyzoites in tissues. Histopathological pro-cessing tracing Sarcocystis and ensuing structural change in the brain tissue were conducted. PCR analysis was conducted on all the brain samples. Sequencing was done for one PCR product. Genotype was identified by Blast search and homology analysis. Result: Sarcocystis spp. was found in one of the brain samples (1.25%) using tis-sue digestion method. The presence of bradyzoite was also confirmed in the pre-pared histopathological sections. PCR analysis was positive in one of samples. Genotyping of one sample proved that Sarcocystis species was Sarcocystis ovicanis and the nucleotide sequence of this parasite was deposited in the GenBank data-base under accession number No.KF489431. Conclusion: Sarcocystis ovicanis can involve brain tissue of sheep and conse-quently causes clinical symptoms.
Frenkel J, Smith D. Determination of the gen-era of cyst–forming coccidian. Parasitol Res. 2003;91:384-389.
Kia EB, Mirhendi H, Rezaeian M, Zahabiun F, Sharbatkhori M. First molecular identification of Sarcocystis miescheraina (Protozoa, Apicom-plexa) from wild boar (Susscrofa) in Iran. Exp Parasitol. 2011;127(3):724-726.
NourollahiFard SR, Asghari M, Nouri F. Sur-vey of Sarcocystis infection in slaughtered cattle in Kerman, Iran. Trop Anim Health Prod. 2009; 41:1633-1636.
Borji H, Azizzadeh M, Kamelli M. A retro-spective study of abattoir condemnation due to parasitic infections: Economic importance in Ahwaz, southwestern Iran. J Parasitol. 2012; 98(5):954-957.
Anja R, Tenter A. Comparison of immunologi-cal and molecular methods for diagnosis of in-fections with pathogenic Sarcocystis species in sheep.Tokai J Exp Clin Med. 1999; 23:293-302.
Hamidinejat H, Hekmatimoghaddam H, Jafari H, Sazmand A, Molayan P, Derakhshan L, Mirabdollahi M. Prevalence and distribution patterns of Sarcocystis in camels in Yazd prov-ince, Iran. J Parasit Dis. 2012;25:1-2.
Dalimi A, Paykari H, Smaeilzade M,Valizade M, Karimi G, Motamedi G, Godarzi M. Detection of Sarcocystis spp of slaughtered sheep in Gazvin Ziaran slaughter house by molecular assay. Mo-dares J Med Sci Pathol. 2008; 11:65-72.
Shahbazi A, Asform Sh, Falah S, Khanmo-hamadi M, Nematollahi A, Moharami T. Iden-tification of Sarcocystis ovicanis and Sarcocystis arti-canis from slaughtered sheep using PCR-RFLP in Tabriz province. J Pathobiol. 2013; 10(2): 959-964.
Munday BL. The effect of Sarcocystis ovicanis on wool growth in sheep. Vet Parasitol. 1984;15: 91-94.
Pereira A, Bermejo M. Prevalence of Sarcocystis cysts in pigs and sheep in Spain. Vet Parasitol. 1988; 27: 353-355.
Pena HF, Ogassawara S, Sinhorini IL. Occur-rence of cattle Sarcocystis spp. in raw kibbe from arabian establishments in the city of Sao Paolo, Brazil and experimental transmission to hu-mans. J Parasitol. 2001;87: 1459-1465.
More G, Abrahamovich P, Jurado S, Baciga-lupe D, Marin JC. Prevalence of Sarcocystis spp. in Argentinean cattle. Vet Parasitol. 2011;177: 162-165.
Britt DP, Baker JR. Causes of death and illness in the native sheep of North Ronaldsay, Ork-ney. I. Adult sheep. Br Vet J. 1990; 146: 129-142. 14. Woldemeskel M, Gebreab F. Prevalence Sarco-cystis in livestock of northwest Ethiopia. Zen-tralbl Vet. 1994; 3: 55-58.
Silva RC, Su C, Langoni H. First identification of Sarcocystis ovicanis (Railliet, 1886) Moulé, 1886 (Protozoa: Apicomplexa) by PCR in naturallinfected sheep from Brazil. Vet Parasitol. 2009;165(3-4):332-6.
Gjerde M. Ultrastructure of the cysts of Sarco-cystis grueneri from cardiac muscle of reindeer (Rangifertarandustarandus). Z Parasitenkd. 1985;71(2):189-198.
Al-Hoot AS, Al-Qureishy SA, Al-Rashid K, Bashtar AR. Microscopic study on Sarcocystis moulei from sheep and goats in Saudi Arabia. J Egypt Soc Parasitol. 2005;35(1):295-312.
Matin S, Nouri A, Azizi H, Nourani H. Preva-lence of thin-walled Sarcocystis cruzi and thick-walled Sarcocystis hirsuta or Sarcocystis hominis from cattle in Iran.Trop Anim Health Prod. 2010;42(6):1225-1227.
Savini G. The epidemiology of Sacocystis in cattle of Western Australia. Epidemiol Infect. 1992;108: 107- 113.
Oryan A, Ahmadi N, Mousavi SM. Prevalence, biology and distribution pattern of Sarcocystis in-fection in water buffalo (Bubalusbubalis) in Iran. Trop Anim Health Prod.2010; 42: 1513-1518.
21.Valinezhad A, Oryan A, Ahmadi N. Sarco-cystis and its complications in camels (Camelusdromedarius) of eastern provinces of Iran. Korean J Parasitol. 2008;46: 229-234.
Shekarforoush M, Shakerian M, Hasanpoor M, Prevalence of Sacocystis in slaughtered one-humped camels in Iran. Trop Anim Health Prod. 2006;38:301-303
O'Toole D, Jeffrey M, Challoner D, Maybey R, Welch V. Ovine myeloencephalitis-leukomye-lomalacia associated with a Sarcocystis-like proto-zoan. J Vet Diagn Invest. 1993; 5: 212-225.
O'Toole D, Duffell SJ, Upcott DH, Frewin D. Experimental microcyst Sarcocystis infection in lambs: pathology.Vet Rec.1988;22(119):525-531.
Cawthorn RJ, Speer CA. Sarcocystis: Infection and disease of humans, livestock, wildlife and other hosts. In: Long PL (Hrsg.): Coccidiosis of man and domestic animals. CRC Press, Bo-ca Raton. 1990: 91-120.
Dubey JP, Speer CA, Fayer R. Sarcocystosis of animals and man. CRC Press, Inc Boca Raton, Florida, 1989.
Donoghue PO, Rommel M. Australian-Ger-man collaborative studies on the immunology of Sarcocystis infections. Angew Parasitol. 1992;33:102-119.
Uggla A, Buxton D. Immune responses against Toxoplasma and Sarcocystis infections in rumi-nants: diagnosis and prospects for vaccination. Rev Sci Tech Off Int Epizoot. 1990; 9: 441-462.
Dubey JP. Lesions in sheep inoculated with Sarcocystis ovicanis sporocysts from canine feces. Vet Parasitol. 1988; 26:237-252.
Scott D, Fitzgerald S, Janovitz E, Kazacos K, Dubey J, Murphy D. Sarcocystosis with in-volvement of the central nervous system in lambs. J Vet Diagn Invest.1993; 5: 291-296
Henderson JM, Dies KH, Haines DM, Higgs GW, Ayroud M. Neurological symptoms asso-ciated with sarcocystosis in adult sheep. Can Vet J. 1997; 38: 168-170.
Caldow GL, Gidlow JR, Schock A. Clinical, pathological and epidemiological findings in three outbreaks of ovine protozoan myeloen-cephalitis. Vet Rec. 2000; 146: 7-10.
Morgan G, Terleki S, Bradley R. A suspected case of Sarcocystis encephalitis in sheep. Br Vet J.1984;140(1):64-69
Sargison N, Schock A, Maclean IA, Rae A, Heckeroth AR, Clark AM. Unusual outbreak of sporozoan encephalomyelitis in Blue faced Leicester ram lambs. Vet Rec. 2000; 146: 225-226.