Genetic Identification of Orientobilharzia turkestanicum from Sheep Isolates in Iran.

  • Reza Tabaripour Dept. of Cellular and Molecular Biology, Islamic Azad University Babol-Branch, Iran.
  • Mohammad Reza Youssefi Dept. of Veterinary Parasitology, Islamic Azad University Babol-Branch, Iran.
  • Rabeeh Tabaripour Young Researchers Club, Islamic Azad University, Babol Branch, Babol, Iran.
Keywords: Iran, Orientobilharzia turkestanicum, Sheep


 Background: Adult worms of Orientobilharzia turkestanicum live in the portal veins, or intestinal veins of cattle, sheep, goat and many other mammals caus-ing orientobilharziasis. Orientobilharziasis causes significant economic losses to livestock industry of Iran. However, there is limited information about genotypes of O. turkestanicum in Iran. Methods:In this study, 30 isolates of O. turkestanicum obtained from sheep were characterized by sequencing mitochondrial cytochrome c oxidase subunit 1 (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) gene. The mitochondrial cox1 and nad1 DNA were amplified by poly-merase chain reaction (PCR) and then sequenced and compared with O. turke-stanicum and that of other members of the Schistosomatidae available in Gen- BankTM.Results:Phylogenetic relationships between them were re-constructed using the maximum parsimony method. Phylogenetic analyses done in present study placed O. turkestanicum within the Schistosoma genus, and indicates that O. turkestanicum was phylogenetically closer to the African schistosome group than to the Asian schistosome group.Conclusion:Comparison of nad1 and cox1 sequences of O. turkestanicum ob-tained in this study with corresponding sequences available in GenbankTM re-vealed some sequence variations and provided evidence for presence of mi-crovarients in Iran.


Farley J. A review of the family Schistoso-matidae: Excluding the genus Schistosoma from mammals. J Helminthol . 1971; 45: 289-320.

Skrjabin KI. Schistosomum turkestanicum, nov. sp., ein neuer parasit des Rinder aus Russich-Turkestan. Ztschr. Infektionskr. u. Haustier 1913; 13: 457-468.

Price EW. A synopsis of the trematode family Schistosomatidae with descriptions of new genera and species. Proc. US Nat Mus Wash. 1929; 75: 1-39.

Dutt SC, Srivastava HD. Revision of the genus Ornithobilharzia odhner, 1912 (Trematoda: Schis-tosomatidae). Proc. 42nd Sci. Congr, 1955: 3: 285.

Soulsby EJ L. Helminths, Arthropods and Pro-tozoa of domestic animals. Published in USA, Williams and Wilkins Company. 1982: 81-83.

Li L, Yu LY, Zhu XQ, Wang CR, Zhai YQ, Zhao JP. Orientobilharzia turkestanicum is grouped within African schistosomes based on phylogenetic analyses using sequences of mito-chondrial genes. Parasitol Res. 2008:102: 939–943.

Yamagiwa S. A study of lesions caused by the invasion of Schistosoma turkestanicum in cattle. J Jap Soc Vet Sci. 1931:2: 131-132.

Tang CT, Tang ZZ, Cao H, Tang L, Cui GW, Qian YC, Lu HC. Studies on the Ornithobilhar-zia turkestanica in sheep in the eastern part of the Inner Mongol Autonomous Region. Acta Zool Sinica. 1983: 29: 249-255.

Motamedi GR, Ghorashi SA, Paykari H, Da-limi AH, Salehi Tabar R, Motamedi N, Karimi GhR. Detection of Ornithobilharzia turkestanikum cercaria (trematoda) by nested-PCR in inter-mediate host snail, Lymnaea gedrosiana. Arch Razi Inst. 2008; 63: 35-40.

Wang CR, Li L, Ni HB, Orientobilharzia turke-stanicum is a member of Schistosoma genus based on phylogenetic analysis using ribosomal DNA sequences. Exp Parasitol. 2009;121 (2): 193–7.

Digentile L, Picot H, Bordeau P, Bardet R, Kerjam A, Piriou M, Leguennic A, Bayssade-Dufour C, Chabasse D, Mott KE. Cercarial dermatitis in Europe: A new public health problem. Bulletin of the World Health Org. 1996; 74: 159-163.

Sahba GH, Malek A. Dermatitis caused by cercariae of Orientobilharzia turkestanicum in the Caspian area of Iran. Am J Trop Med Hyg. 1979; 28: 912–913.

Montgomery R E. Observations on bilharziosis among animals in India. J Trop Vet Sci 1906;1: 138-174.

Hsü ST, Yang P. A preliminary study on blood flukes of cattle and sheep in Kansu province, including descriptions of a new species. Acta Vet. 1957; 2: 117-124.

Dutt SC, Srivastava HD. On the morphology and life history of a new mammalian blood–fluke Ornithobilharzia dattai n. sp. Parasitology. 1952; 42:144-150.

Kruatrachue M, Bhaibulaya M, Harinasuta C. Ornithobilharzia harinasutai sp. nov., a mamma-lian blood-fluke, its morphology and life-cycle. Ann Trop Med Parasitol. 1965; 59: 181-188.

Bhalerao GD. On the identity of the schisto-some found in cases of bovine nasal granuloma and some observations on a few other mem-bers of the Schistosomatidae. Ind J Vet Sci Anim Husb. 1932; 2: 338-356.

Dutt SC, Srivastava HD. A revision of the ge-nus Ornithobilharzia odhner (1912), with the crea-tion of two genera Orientobilharzia dutt and sri-vastava (1955) and Sinobilharzia dutt and srivastava (1955) (Trematoda: Schistosomatidae). Ind J Helminthol. 1961; 13: 61-73.

Massoud J. The effect of variation in miracidial exposure dose on laboratory infections of Orni-thobilharzia turkestanicum in lymnaea gedrosiana. J Helminthology. 1974; 48: 139-144.

Eslami A. Trematoda Veterinary Helminthol-ogy. Tehran University Publications. 1990; 116-146.

Wang Y, Wang CR, Zhao GH, Gao JF, Li MW, Zhu XQ. The complete mitochondrial genome of Orientobilharzia turkestanicum sup-ports its affinity with African Schistosoma spp. Infection, Genetics and Evolution. 2011; 11: 1964-1970.

Youssefi MR, Tabaripour R, Fallah-Omrani V, Spotin A, Esfandiari B. Genotypic characteri-zation of Echinococcus granulosus in Iranian goats. Asian Pac J Trop Dis. 2013; 3(5): 362-366.

Sánchez E, Cáceres O, Náquira C, Garcia D, Patiño G, Silvia H, Volotão AC, Fernandes O. Molecular characterization of Echinococcus granu-losus from Peru by sequencing of the mitochon-drial cytochrome C oxidase subunit 1 gene. Mem Inst Oswaldo Cruz, 2010; 105(6): 806-810.

Vural G, Baca AU, Gauci CG, Bagci O, Gicik, Y, Lightowlers MW. Variability in the Echinococ-cus granulosus Cytochrome C oxidase 1 mito-chondrial gene sequence from livestock in Turkey and a re-appraisal of the G1–3 geno-type cluster. Vet Parasitol. 2008; 154: 347–350.

Snyder SD, Loker ES. Evolutionary relation-ships among the Schistosomatidae (Platyhel-minthes: Digenea) and an asian origin for Schis-tosoma. J Parasitol. 2000; 86(2): 283-288.

Attwood SW, Upatham ES, Meng XH, Qiu DC, Southgate VR, The phylogeography of Asian Schistosoma (Trematoda: Schistoso-matidae). Parasitology. 2002; 125: 99–112.

Lockyer AE, Olson PD, Ostergaard P , Rollin-son D, Johnston DA, Attwood SW, Southgate et al. The phylogeny of the Schistosomatidae based on three genes with emphasis on the in-terrelationships of Schistosoma Weinland, 1858. Parasitology. 2003; 126: 203–224.

Brant SV, Loker ES, Can specialized pathogens colonize distantly related hosts? Schistosome evo-lution as a case study. PLoS Path. 2005; 1: e38.

Webster BL, Southgate VR, Littlewood DT, A revision of the interrelationships of Schistosoma including the recently described Schistosoma guin-eensis. Int J Parasitol. 2006; 36: 947–955.

How to Cite
Tabaripour R, Youssefi MR, Tabaripour R. Genetic Identification of Orientobilharzia turkestanicum from Sheep Isolates in Iran. Iran J Parasitol. 10(1):62-8.