Cloning and Expression of Recombinant Plasmid Containing P36/LACK Gene of Leishmania infantum Iranian Strain.

  • Saloomeh Shirali Dept. of Biology, Faculty of Basic science, Ahvaz branch, Islamic Azad University, Ahvaz, Iran AND Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Hamidreza Haddadzadeh Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Mehdi Mohebali Dept. of Medical Parasitology and Mycology, School of Public health, Tehran University of Medical Sciences, Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
  • Bahram Kazemi Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Dept. of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Narges Amini Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Keywords: Cloning, Expression, LACK, Leishmania infantum, Visceral leishmaniasis


Background:There are several methods, such as vaccination, to control visceral leishmaniasis. Although there is no efficient vaccine, it seem DNA vaccination with stimulates both cellular and humoral immunity apparently is the best way. The aim of this study was cloning and expression of LACK gene, a 36kD protein, as a can-didate protein for vaccination against Iranian L.infantum.Methods:Iranian strain of L. infantum [MCAN/IR/07/Moheb-gh] was used as a template for PCR to amplify LACK gene. The LACK gene was cloned in pTZ57R/T vector and after confirmation it was digested by restriction enzymes (BamH1) and cloned in pcDNA3.1 expression vector. Recombinant plasmid was extracted and analyzed by sequencing, restriction digestion analysis and PCR reac-tion. The pc- LACK recombinant plasmid was purified from transformed E.coli (DH5α) and its expression was analyzed by SDS-PAGE and Western blot.Results:The results of sequencing, restriction digestion analysis and PCR reaction revealed that LACK gene was cloned correctly in pcDNA3.1 vector and the results of SDS PAGE and Western blot emphasized that LACK protein of Iranian L. in-fantum is a well-expressed protein. Conclusion:We amplified, cloned and expressed Iranian L. infantum LACK genes successfully.


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How to Cite
Shirali S, Haddadzadeh H, Mohebali M, Kazemi B, Amini N. Cloning and Expression of Recombinant Plasmid Containing P36/LACK Gene of Leishmania infantum Iranian Strain. Iran J Parasitol. 10(2):164-170.
Original Article(s)