Phylogenetic Study of Haemonchus Species from Iran Based On Morpho-Molecular Characterization.

  • Behnam Meshgi Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes).
  • Fatemeh Jalousian Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes).
  • Zahra Masih Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes).
Keywords: Haemonchus species, ITS2, Morphology, PCR-RFLP

Abstract

Background:Haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. The present study was carried out to differentiate Haemonchus species from its main hosts in Iran, including sheep, goat and camel. Methods:The identification took place based on the morphometrics of the spic-ules and molecular characters. Two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each ani-mal) at the slaughterhouses from different localities in Iran. Samples were morpho-logically identified according to the spicules’ morphometric measurements. In the section on molecular study, 10 samples of each Haemonchus isolates were genetically examined. A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results.Results:PCR-RFLP profile obtained from the restriction enzyme HPa1 in H. con-tortus and H. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different frag-ments, respectively. The morphological parameters clearly distinguish H. contortus from H. longistipes. Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively.Conclusion:The genotypic results are in agreement with the phenotypic findings of both species.

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How to Cite
1.
Meshgi B, Jalousian F, Masih Z. Phylogenetic Study of Haemonchus Species from Iran Based On Morpho-Molecular Characterization. IJPA. 10(2):189-96.
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