Iranian Journal of Parasitology 2015. 10(3):498-504.

The effects of Ostertagia occidentalis somatic antigens on ovine TLR2 and TLR4 expression
Hassan BORJI, Alireza HAGHPARAST, Nooshinmehr SOLEIMANI, Mohammad AZIZZADEH, Mohammad Hossein NAZEMSHIRAZI


Background: Recognition of helminth-derived pathogen associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs), including toll like recep­tors (TLRs) is the first step towards initiating anti–helminth immune re­sponses.

Methods: Using somatic antigens of Ostertagia occidentalis, an important abomasal parasite of ruminants, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analyzed by real-time quatitative reverse-transcrip­tion polymerase chain reaction (qRT-PCR). Somatic antigens of O. occidentalis were prepared to stimulate ovine PBMCs in a time and dose dependent manner.

Results: A high expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of the parasites specially when PBMCs were cultured with 100 µg/ml of somatic antigens and incubated for 2h. Up-regulation of TLR2 expres­sion was more pronounced and evident in our study.

Conclsusion: Somatic antigens of O. occidentalis have immunostimulatory and domi­nant role on peripheral immune cells. This study provide for the first time evidence of induction of TLRs in ovine PBMCs by somatic antigen of O. occidentalis


Ostertagia occidentalis; Peripheral blood mononuclear cells; Toll-like receptors

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