The effects of Ostertagia occidentalis somatic antigens on ovine TLR2 and TLR4 expression

  • Hassan BORJI Dept. of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Alireza HAGHPARAST Dept. of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Nooshinmehr SOLEIMANI Dept. of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Mohammad AZIZZADEH Dept. of Clinical Science, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Mohammad Hossein NAZEMSHIRAZI Central Laboratories of Khorasan Razavi Veterinary Organization, Mashhad, Iran
Keywords: Ostertagia occidentalis, Peripheral blood mononuclear cells, Toll-like receptors


Background: Recognition of helminth-derived pathogen associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs), including toll like recep­tors (TLRs) is the first step towards initiating anti–helminth immune re­sponses.Methods: Using somatic antigens of Ostertagia occidentalis, an important abomasal parasite of ruminants, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analyzed by real-time quatitative reverse-transcrip­tion polymerase chain reaction (qRT-PCR). Somatic antigens of O. occidentalis were prepared to stimulate ovine PBMCs in a time and dose dependent manner.Results: A high expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of the parasites specially when PBMCs were cultured with 100 µg/ml of somatic antigens and incubated for 2h. Up-regulation of TLR2 expres­sion was more pronounced and evident in our study.Conclsusion: Somatic antigens of O. occidentalis have immunostimulatory and domi­nant role on peripheral immune cells. This study provide for the first time evidence of induction of TLRs in ovine PBMCs by somatic antigen of O. occidentalis


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How to Cite
BORJI H, HAGHPARAST A, SOLEIMANI N, AZIZZADEH M, NAZEMSHIRAZI MH. The effects of Ostertagia occidentalis somatic antigens on ovine TLR2 and TLR4 expression. IJPA. 10(3):498-04.
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