Iranian Journal of Parasitology 2015. 10(3):465-472.

Genotyping Characterization of Toxoplasma gondii in Cattle, Sheep, Goats and Swine from the North of Portugal
Ana Patrícia LOPES, Anabela VILARES, Francisco NETO, Alcina RODRIGUES, Tânia MARTINS, Idalina FERREIRA, Maria João GARGATÉ, Manuela RODRIGUES, Luís CARDOSO


Background: Epidemiological investigations on Toxoplasma gondii infection have found a significant association between human toxoplasmosis and consumption of raw or undercooked meat. The present study aimed to characterize genotypes of T. gondii in 20 cattle, 40 sheep, 15 goats and 16 pigs from the North of Portugal.

Methods: Nested PCR amplified the surface antigen 2 (SAG2) gene. Sequencing analy­sis was performed in order to assess the prevalence of SAG2 type strains (I, II and III).

Results: Three and 4 strains of SAG2 type II were identified in heart samples of cattle and sheep, respectively. Three SAG2 type II strains were detected in brain, diaphragm and heart of 3 pigs. Three strains detected in heart samples of 3 goats belonged to SAG2 types I or II; with the same result being observed in heart samples from 2 sheep and in 2 brain and 1 heart samples from 3 pigs.

Conclusion: SAG2 type II has been shown for the first time to infect cattle in North of Portugal. In addition, SAG2 type II has also been confirmed as the predominant strain in sheep and pigs in the same region. This is the first molecular report of T. gondii in goats from Portugal.


Genotypes; Portugal; Ruminants; Swine; Toxoplasma gon-dii

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