Genotyping Characterization of Toxoplasma gondii in Cattle, Sheep, Goats and Swine from the North of Portugal

  • Ana Patrícia LOPES Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal AND Animal and Veterinary Research Centre (CECAV), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal
  • Anabela VILARES Department of Infectious Diseases, National Institute of Health, Lisbon, Portugal
  • Francisco NETO Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal
  • Alcina RODRIGUES Veterinary Services Directorate of North Region, Veterinary General-Directorate, Ministry of Agriculture, Portugal
  • Tânia MARTINS Veterinary Services Directorate of North Region, Veterinary General-Directorate, Ministry of Agriculture, Portugal
  • Idalina FERREIRA Department of Infectious Diseases, National Institute of Health, Lisbon, Portugal
  • Maria João GARGATÉ Department of Infectious Diseases, National Institute of Health, Lisbon, Portugal
  • Manuela RODRIGUES Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal AND Animal and Veterinary Research Centre (CECAV), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal
  • Luís CARDOSO Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal AND Parasite Disease Group, Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Portugal
Keywords: Genotypes, Portugal, Ruminants, Swine, Toxoplasma gon-dii

Abstract

Background: Epidemiological investigations on Toxoplasma gondii infection have found a significant association between human toxoplasmosis and consumption of raw or undercooked meat. The present study aimed to characterize genotypes of T. gondii in 20 cattle, 40 sheep, 15 goats and 16 pigs from the North of Portugal.Methods: Nested PCR amplified the surface antigen 2 (SAG2) gene. Sequencing analy­sis was performed in order to assess the prevalence of SAG2 type strains (I, II and III).Results: Three and 4 strains of SAG2 type II were identified in heart samples of cattle and sheep, respectively. Three SAG2 type II strains were detected in brain, diaphragm and heart of 3 pigs. Three strains detected in heart samples of 3 goats belonged to SAG2 types I or II; with the same result being observed in heart samples from 2 sheep and in 2 brain and 1 heart samples from 3 pigs.Conclusion: SAG2 type II has been shown for the first time to infect cattle in North of Portugal. In addition, SAG2 type II has also been confirmed as the predominant strain in sheep and pigs in the same region. This is the first molecular report of T. gondii in goats from Portugal.

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Published
2015-10-17
How to Cite
1.
LOPES AP, VILARES A, NETO F, RODRIGUES A, MARTINS T, FERREIRA I, GARGATÉ MJ, RODRIGUES M, CARDOSO L. Genotyping Characterization of Toxoplasma gondii in Cattle, Sheep, Goats and Swine from the North of Portugal. IJPA. 10(3):465-72.
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Original Article(s)