Iranian Journal of Parasitology 2015. 10(3):381-388.

Molecular Detection and Identification of Zoonotic Microspor-idia Spore in Fecal Samples of Some Animals with Close-Con-tact to Human
Zeinab ASKARI, Hamed MIRJALALI, Mehdi MOHEBALI, Zabih ZAREI, Saeideh SHOJAEI, Tahereh REZAEIAN, Mostafa REZAEIAN

Abstract


Background: Microsporidia species are obligatory intracellular agents that can in­fect all major animal groups including mammals, birds, fishes and insects. Whereas world­wide human infection reports are increasing, the cognition of sources of infec­tion particularly zoonotic transmission could be helpful. We aimed to detect zoono­tic microsporidia spore in fecal samples from some animals with close – contact to human.

Methods: Overall, 142 fecal samples were collected from animals with closed-con­tact to human, during 2012-2013. Trichrome – blue staining were performed and DNA was then extracted from samples, identified positive, microscopically. Nested PCR was also carried out with primers targeting SSU rRNA gene and PCR products were sequenced.

Results: From 142 stool samples, microsporidia spores have been observed microscopi­cally in 15 (10.56%) samples. En. cuniculi was found in the faces of 3 (15%) small white mice and 1 (10%) laboratory rabbits(totally 2.81%). Moreover, E. bieneusi was detected in 3 (10%) samples of sheep, 2 (5.12%) cattle, 1 (10%) rabbit, 3 (11.53%) cats and 2 (11.76%) ownership dogs (totally 7.74%). Phylogenetic analysis showed interesting data. This is the first study in Iran, which identified E. bieneusi and En. Cuniculi in fecal samples of laboratory animals with close – contact to human as well as domesticated animal and analyzed them in phylogenetic tree.

Conclusion: E. bieneusi is the most prevalent microsporidia species in animals. Our results can also alert us about potentially zoonotic transmission of microsporidiosis.

Keywords


Laboratory animals , Enterocytozoon bieneusi , Encephalitozoon cuniculi , Zoonotic transmission

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References


Sprague V, Becnel JJ , Hazard EI. Taxonomy of phylum microspora. Crit Rev Microbiol. 1992; 18:285-395.

Canning EU, Lom J, Dykova I. The microsporidia of vertebrates. JBM .1986; 27 (5)

Wittner M. Historic perspective on the microsporidia: expanding horizons, in The Microsporidia and Microsporidiosis, Wittner M and Weiss L, Editors, American Society of Microbiology. 1999; Washington DC.

Matsubayashi H, Koike I, Mikata I, Takei H, Hagiwara S. A case of Encephalitozoon-like infection in man. Arch Pathol. 1959; 67:181-7.

Didier ES, Stovall ME, Green LC et al. Epidemiology of microsporidiosis: sources and modes of transmission. Vet Parasitol. 2004; 126 (1-2):145-66.

Mathis A, Weber R, Deplazes P. Zoonotic potential of the microsporidia. Clin Microbiol Rev. 2005; 18 (3):423-45.

Mathis A, Breitenmoser AC, Deplazes P. Detection of new Enterocytozoon genotypes in faecal samples of farm dogs and a cat. Parasite. 1999; 6 (2):189-93.

Haro M, Izquierdo F, Henriques-Gil N, et al. First detection and genotyping of human-associated microsporidia in pigeons from urban parks. Appl Environ Microbiol. 2005; 71 (6):3153-7.

Fayer R, Santin MJ, Trout M. First detection of microsporidia in dairy calves in North America. Parasitol Res. 2003; 90 (5):383-6.

Sak B, Kvac M, Hanzlikova D, Cama V. First report of Enterocytozoon bieneusi infection on a pig farm in the Czech Republic. Vet Parasitol. 2008; 153 (3-4):220-4.

Lores B, Del Aguila C, Arias C. Enterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from Galicia, Spain. Mem Inst Oswaldo Cruz. 2002; 97 (7):941-5.

Deplazes P, Mathis A, Baumgartner R, Tanner I, Weber R. Immunologic and molecular characteristics of Encephalitozoon-like mic-rosporidia isolated from humans and rabbits indicate that Encephalitozoon cuniculi is a zoonotic parasite. Clin Infect Dis. 1996; 22 (3):557-9.

Thomas C, Finn M, Twigg L, Deplazes P, Thompson RC. Microsporidia (Enceph-alitozoon cuniculi) in wild rabbits in Australia. Aust Vet J. 1997; 75 (11):808-10.

Jamshidi S, Tabrizi AS, Bahrami M, Momtaz H. Microsporidia in household dogs and cats in Iran; a zoonotic concern. Vet Parasitol. 2012; 185 (2-4):121-3.

Slodkowicz-Kowalska A. Animal reservoirs of human virulent microsporidian species. Wiad Parazytol. 2009; 55 (1):63-5.

Black SS, Steinohrt LA, Bertucci DC, Rogers LB, Didier ES. Encephalitozoon hellem in budgerigars (Melopsittacus undulatus). Vet Pathol. 1997; 34 (3):189-98.

Sak B, Kasickova D, Kvac M, Kvetonova D, Ditrich O. Microsporidia in exotic birds: intermittent spore excretion of Encephalitozoon spp. in naturally infected budgerigars (Melopsittacus undulatus). Vet Parasitol. 2010; 168 (3-4):196-200.

Abe N, Kimata I. Molecular survey of Enterocytozoon bieneusi in a Japanese porcine population. Vector Borne Zoonotic Dis. 2010; 10 (4):425-7.

Pirestani M, Sadraei J, Forouzandeh M. Molecular characterization and genotyping of human related microsporidia in free-ranging and captive pigeons of Tehran, Iran. Infection, Genetics and Evolution. 2013; 20 (0):495-499.

Mirjalali H, Mohebali M, Mirhendi H et al. Emerging intestinal microsporidial infection in HIV+/AIDS patients in Iran: microscopical and molecular detection. Iran J Parasitol. 2014; 9 (2):149-154

Ryan NJ, Sutherland G, Coughlan K et al. A new trichrome-blue stain for detection of microsporidial species in urine, stool, and nasopharyngeal specimens. J Clin Microbiol. 1993; 31 (12):3264-9.

Didier ES. Microsporidiosis: an emerging and opportunistic infection in humans and animals. Acta Trop. 2005; 94 (1):61-76.

Dascomb K, Frazer T, Clark RA, Kissinger P, Didier E. Microsporidiosis and HIV. J Acquir Immune Defic Syndr. 2000; 24 (3):290-2.

Franzen C, Muller A. Microsporidiosis: human diseases and diagnosis. Microbes Infect. 2001; 3:389-400.

Hoffman RM, Wolk DM, Spencer SK, Borchardt MA. Development of a method for the detection of waterborne microsporidia. J Microbiol Methods. 2007; 70 (2):312-8.

Decraene V, Lebbad M, Botero-Kleiven S, Gustavsson AM, Lofdahl M. First reported foodborne outbreak associated with microspo-ridia, Sweden, October 2009. Epidemiol Infect. 2011; 1-9.

Deplazes P, Mathis A, Weber R. Epidemiology and zoonotic aspects of microsporidia of mammals and birds.

Agholi M, Hatam GR, Motazedian MH. Microsporidia and coccidia as causes of persistence diarrhea among liver transplant children: incidence rate and species/genotypes. Pediatr Infect Dis J. 2013; 32 (2):185-7.

Agholi M, Hatam GR, Motazedian MH. HIV/AIDS-associated opportunistic protozoal diarrhea. AIDS Res Hum Retroviruses. 2013; 29 (1):35-41.

Wasson K, Peper RL. Mammalian microspo-ridiosis. Vet Pathol. 2000; 37 (2):113-28.

Sak B, Ditrich O. Humoral intestinal immunity against Encephalitozoon cuniculi (Microsporidia)

infection in mice. Folia Parasitol (Praha). 2005; 52 (1-2):158-62.

Tay WT, O'Mahony EM, Paxton RJ. Complete rRNA gene sequences reveal that the microsporidium Nosema bombi infects diverse bumblebee (Bombus spp.) hosts and contains multiple polymorphic sites. J Eukaryot Microbiol. 2005; 52 (6):505-13.

Dong SN, Shen ZY, Xu L, Zhu F. Sequence and phylogenetic analysis of SSU rRNA gene of five microsporidia. Curr Microbiol. 2010; 60 (1):30-7.


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