Iranian Journal of Parasitology 2018. 13(2):244-250.

Re-record and Spore Ultrastructure of Nosema melasomae Si-dor & Jodal 1986, a Microsporidian Pathogen of Crysomela populi (Coleoptera: Chrysomelidae)
Mustafa YAMAN


Background: Chrysomela (=Melasoma) populi is one of the most serious pests on poplar plantations. In the present study, a microsporidian pathogen, Nosema melasomae infecting Crysomela populi is re-recorded from a new geographical locality and its spore ultrastructure is given for the first time.

Methods: Larvae and adults of C. populi were dissected in Ringer’s solution and prepared wet smears were examined under a microscope. Detected fresh and stained spores were measured and photographed using an Olympus BX51 microscope with a DP-25 digital camera and a DP2-BSW Soft Imaging System. The ultrastructure of the pathogen was studied with a Philips EM 208 transmission electron microscope using standard preparation techniques as previously described

Results: Fresh spores of the microsporidian pathogen are elongate, 4.86 ± 0.71 µm in length and 1.64 ± 0.19 µm in width. The spore wall is considerable thin, measured 60 to 100 nm and consists of a clear endospore (40 to 80 nm) and an electron-dense, uniform exospore (15 to 30 nm). The polar filament is isofilar and has only 6-8 coils. Nuclei in the cell are 400-560 nm in diameter. The polaroplast has a thin lamellated structure.

Conclusion: The pathogen from C. populi is N. melasomae Sidor & Jodal, 1986 and its systematic position given by Sidor and Jodal. The spore ultrastructure of N. melasomae differs from those of other microsporidia infecting chrysomelids.


Nosema melasomae, Sporeultrastructure, Mi-crosporidium, Crysomela populi, Chrysomelidae

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Urban J. Occurrence, bionomics and harmfulness of Chrysomela populi L. (Coleoptera; Chrysomelidae). J Forest Sci. 2006; 52: 255–284.

Yaman M, Radek R. Nosema chaetocnemae sp. n., a microsporidian (Microspora; Nosematidae) parasite of Chaetocnema tibialis (Chrysomelidae, Coleoptera). Acta Protozool. 2003; 42: 231-237.

Yaman M, Radek R, Aslan I, Ertürk Ö. Characteristic features of Nosema phyllotretae Weiser 1961, a microsporidian parasite of Phyllotreta atra (Coleoptera: Chrysomelidae) in Turkey. Zool Stud. 2005; 44: 368-372.

Yaman M, Radek R, Toguebaye B. A new microsporidian of the genus Nosema, parasite of Chaetocnema tibialis (Coleoptera: Chrysomelidae) from Turkey. Acta Protozool. 2008; 47: 279-285.

Yaman M, Radek R, Weiser J, Toguebaye BS. Unikaryon phyllotretae sp. n. (Protista, Microspora), a new microsporidian pathogen of Phyllotreta undulata (Coleoptera; Chrysomelidae). Eur J Protistol. 2010; 46(1):10-6.

Yaman M, Özcan N, Radek R, Linde A, Lipa JJ. Ultrastructure, characteristic features and occurrence of Nosema leptinotarsae Lipa 1968, a microsporidian pathogen of Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Acta Parasitol. 2011; 56: 1-7.

Zhu F, Shen Z, Guo X, Xu X, Tao H, Tang X, Xu L. A new isolate of Nosema sp. (Microsporidia, Nosematidae) from Phyllobrotica armata Baly (Coleoptera, Chrysomelidae) from China. J Invertebr Pathol. 2011; 106(2):339-42.

Toguebaye BS, Marchand B, Bouix G. Microsporidia of Chrysomelidae. In: Petitpierre E, Hsiao TH, Jolivet PH editors. Biology of Chrysomelidae. Kluwer Academic Publishers, Boston, pp. 1988; 399-416.

Sidor C, Jodal I. Nosema melasomae causing a disease of the poplar leaf beetle (Melasoma populi L., Chrysomelidae, Coleoptera). Zaštita Bilja 1986; 37: 243–249.

Zeki H, Toros S. The effect of host on the adults of Chrysomela populi L. and Chrysomela tremulae F. (Col.:Chrysomelidae). Bitki Koruma Bülteni 1996; 36: 25–38.

Yaman M. Distribution and occurrence of the neogregarine pathogen, Ophryocystis anatoliensis (Apicomplexa) in populations of Chrysomela populi L. (Coleoptera: Chrysomelidae). Acta Protozool. 2017; 56: 283–288.

Vávra J, Larsson R. Structure of Microsporidia. In: Wittner M editor. The Microsporidia and Microsporidiosis. ASM Press, Washington, 1999; pp. 7-84.

Larsson J I R. Identification of microsporidia. Acta Protozool. 1999; 38: 161-197.

Yaman M, Pınar Güngör F, Gonca Güner B, Radek R, Linde A. First report and spore ultrastructure of Vairimorpha plodiae (Opisthokonta: Microspora) from Plodia interpunctella (Lepidoptera: Pyralidae) in Turkey. Acta Parasitol. 2016; 61(2):228-31.

Cheung WWK, Wang JB. Electron microscopic studies on Nosema mesnili Paillot (Microsporidia: Nosematidae) infecting the Malpighian tubules of Pieris canidia larva. Protoplasma 1995;186: 142-148.

Toguebaye BS, Marchand B. Nosema couilloudi n.sp., Microsporidie parasite de Nisotra sp. (Coleoptera, Chrysomelidae): Cytopathologie et ultrastructure des stades de developpement. Protistologica, 1984; 20: 357-365.

Toguebaye BS, Marchand B. Etude d'une infection microsporidienne due à Nosema birgii n.sp. (Microsporida, Nosematidae) chez Mesoplatys cincta Olivier, 1790 (Coleoptera, Chrysomelidae). Z Parasitenkd. 1986; 72: 723-737.

Toguebaye BS, Marchand B. Observations en microscopie électronique à transmission des stades de développement de Nosema nisotrae sp.n. (Microsporida, Nosematidae) parasite de Nisotra sp. (Coleoptera, Chrysomelidae). Arch Protistenkd. 1989; 137: 69-80.

Toguebaye BS, Bouix G. Nosema galerucellae sp.n., microsporidian (Protozoa, Microspora), parasite of Galerucella luteola Müller (Chrysomelidae, Coleoptera): Development cycle and ultrastructure. Eur J Protistol. 1989; 24: 346-353.

Weiser J. Die Mikrosporidien als Parasiten der Insekten. Monogr Angew Entomol. 1961; 17: 1-149.

Hostounský Z, Weiser J. Nosema gastroideae sp. n. (Nosematidae, Microsporidia) infecting Gastrophysa polygoni and Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Acta Entomol Bohemoslov. 1973; 70: 345-350.

Hostounský Z, Weiser J. Nosema polygrammae sp. n. and Plistophora fidelis sp. n. (Microsporidia, Nosematidae) infecting Polygramma undecimlineata (Coleoptera: Chrysomelidae) in Cuba. - Vestn Cesk Spol Zool. 1975; 39: 104-110.

Hostounský Z, Weiser J. A microsporidian infection in Otiorrhynchus equestris (Coleoptera, Curculionidae) in Cuba. Vestn Cesk Spol Zool. 1980; 44: 160–165.

Lipa JJ. Nosema leptinotarsae sp.n., a microsporidian parasite of the Colorado potato beetle, Leptinotarsa decemlineata (Say). J Inverteb. Pathol. 1968; 10: 111-115.


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